Influence of DNA extraction method, 16S rRNA targeted hypervariable regions, and sample origin on microbial diversity detected by 454 pyrosequencing in marine chemosynthetic ecosystems

Applied and Environmental Microbiology

Volumn 80, Issue 15, 2014, Pages 4626-4639

  Cruaud, Perrine ^a,b   Vigneron, Adrien ^a,b   Lucchetti Miganeh, Céline ^c   Ciron, Pierre Emmanuel ^c   Godfroy, Anne ^a,b   Cambon Bonavita, Marie Anne ^a,b  

^a Centre National de la Recherche Scientifique   (France)

^b UNIVERSITÉ DE BRETAGNE OCCIDENTALE   (France)

^c Genostar   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; FISH; FLUORESCENCE MICROSCOPY; HOT SPRINGS; POLYMERASE CHAIN REACTION; RNA; SUBMARINE GEOLOGY;

COMMUNITY COMPOSITION; DIFFERENT PROPORTIONS; DIVERSITY ANALYSIS; FLUORESCENCE IN SITU HYBRIDIZATION; MICROBIAL COMMUNITIES; MICROBIAL DIVERSITY; NEXT-GENERATION SEQUENCING; OPERATIONAL TAXONOMIC UNITS;

EXTRACTION;

ARCHAEAL DNA; BACTERIAL DNA; PRIMER DNA; RNA 16S; SEA WATER;

ANALYSIS; ARCHAEON; BACTERIUM; BIODIVERSITY; CLASSIFICATION; ECOSYSTEM; EVALUATION STUDY; GENETICS; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; PROCEDURES; SEDIMENT;

ANALYTIC SAMPLE PREPARATION METHODS; ARCHAEA; BACTERIA; BIODIVERSITY; DNA PRIMERS; DNA, ARCHAEAL; DNA, BACTERIAL; ECOSYSTEM; GEOLOGIC SEDIMENTS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MOLECULAR SEQUENCE DATA; PHYLOGENY; RNA, RIBOSOMAL, 16S; SEAWATER;

EID: 84904893889     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00592-14     Document Type: Article

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