Commercial DNA extraction kits impact observed microbial community composition in permafrost samples

FEMS Microbiology Ecology

Volumn 87, Issue 1, 2014, Pages 217-230

  Vishnivetskaya, Tatiana A ^a   Layton, Alice C ^a   Lau, Maggie C Y ^b   Chauhan, Archana ^a   Cheng, Karen R ^a   Meyers, Arthur J ^a   Murphy, Jasity R ^a   Rogers, Alexandra W ^a   Saarunya, Geetha S ^a   Williams, Daniel E ^a   Pfiffner, Susan M ^a   Biggerstaff, John P ^a   Stackhouse, Brandon T ^b   Phelps, Tommy J ^c   Whyte, Lyle ^d   Sayler, Gary S ^a   Onstott, Tullis C ^b  

^a UNIVERSITY OF TENNESSEE   (United States)

^b PRINCETON UNIVERSITY   (United States)

^c OAK RIDGE NATIONAL LABORATORY   (United States)

^d MCGILL UNIVERSITY   (Canada)

Author keywords

Bacteria; DNA; Fluorescent in situ hybridization; MCherry seeded approach; Pyrosequences; QPCR

Indexed keywords

BACTERIOLOGY; BACTERIUM; CARBON CYCLE; COMMUNITY COMPOSITION; DNA; EXTRACTION METHOD; GENOMICS; LYSIS; MICROBIAL COMMUNITY; PERMAFROST;

ACIDOBACTERIA; ACTINOBACTERIA; BACTERIA (MICROORGANISMS); FIRMICUTES; GAMMAPROTEOBACTERIA; GEMMATIMONADETES; METHYLOCYSTACEAE; PROTEOBACTERIA;

BACTERIAL DNA; DNA; RNA 16S;

ARCTIC; ARTICLE; BACTERIUM; CHEMISTRY; CLASSIFICATION; DIAGNOSTIC KIT; ECONOMICS; EVALUATION STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GENETICS; ISOLATION AND PURIFICATION; MCHERRY SEEDED APPROACH; METHODOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; PYROSEQUENCES; QPCR;

BACTERIA; DNA; FLUORESCENT IN SITU HYBRIDIZATION; MCHERRY SEEDED APPROACH; PYROSEQUENCES; QPCR;

ARCTIC REGIONS; BACTERIA; DNA, BACTERIAL; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY;

EID: 84891496235     PISSN: 01686496     EISSN: 15746941     Source Type: Journal    
DOI: 10.1111/1574-6941.12219     Document Type: Article

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