Hot-alkaline DNA extraction method for deep-subseafloor archaeal communities

Applied and Environmental Microbiology

Volumn 80, Issue 6, 2014, Pages 1985-1994

  Morono, Yuki ^a   Terada, Takeshi ^b   Hoshino, Tatsuhiko ^a   Inagaki, Fumio ^a  

^a JAPAN AGENCY FOR MARINE EARTH SCIENCE AND TECHNOLOGY   (Japan)

^b MARINE WORKS JAPAN LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE TREATMENT; CELL DISRUPTION EFFICIENCY; CONVENTIONAL METHODS; MICROBIAL COMMUNITIES; MICROBIAL COMMUNITY ANALYSIS; MOLECULAR ANALYSIS; SUBSEAFLOOR SEDIMENTS; TEMPERATURE CONDITIONS;

CELLS; CYTOLOGY; DNA; GENES; MICROORGANISMS; OPTIMIZATION; RNA; SEDIMENTS;

POLYMERASE CHAIN REACTION;

DEEP-SEA SEDIMENT; MICROBIAL COMMUNITY; MITOCHONDRIAL DNA; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; PROKARYOTE; REAL TIME; SEAFLOOR;

ARCHAEAL DNA; RNA 16S; SODIUM HYDROXIDE;

ARCHAEON; ARTICLE; CHEMISTRY; DNA SEQUENCE; GENETICS; HEAT; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; MICROBIOLOGY; MOLECULAR BIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; SEDIMENT; STANDARD; TIME;

ARCHAEA; DNA, ARCHAEAL; GEOLOGIC SEDIMENTS; HOT TEMPERATURE; MOLECULAR BIOLOGY; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SODIUM HYDROXIDE; SPECIMEN HANDLING; TIME FACTORS;

EID: 84897672150     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.04150-13     Document Type: Article

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