Assessment of bacterial community structure in the deep sub-seafloor biosphere by 16S rDNA-based techniques: A cautionary tale

Journal of Microbiological Methods

Volumn 55, Issue 1, 2003, Pages 155-164

  Webster, Gordon ^a   Newberry, Carole J ^a   Fry, John C ^a   Weightman, Andrew J ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

16S rRNA; Anaerobic sediments; Deep biosphere; DGGE; DNA extraction; PCR bias

Indexed keywords

RIBOSOME DNA; RIBOSOME RNA;

ANALYTICAL EQUIPMENT; ARTICLE; BACTERIAL FLORA; BIOSPHERE; CONTROLLED STUDY; DEEP SEA; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA DETERMINATION; DNA EXTRACTION; DNA TEMPLATE; DRILL; GENE AMPLIFICATION; GENETIC VARIABILITY; GEOGRAPHY; INTERMETHOD COMPARISON; LABORATORY; MICROBIAL BIOMASS; MICROBIAL DIVERSITY; MOLECULAR ECOLOGY; NONHUMAN; PACIFIC OCEAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RANDOMIZATION; SAMPLE; SEDIMENT; STANDARD; STRUCTURE ANALYSIS; TECHNIQUE;

BACTERIA (MICROORGANISMS);

EID: 0142091782     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(03)00140-4     Document Type: Article

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