Quantitative PCR methods for RNA and DNA in marine sediments: Maximizing yield while overcoming inhibition

FEMS Microbiology Ecology

Volumn 72, Issue 1, 2010, Pages 143-151

  Lloyd, Karen G ^a   MacGregor, Barbara J ^a   Teske, Andreas ^a  

^a University of North Carolina at Chapel Hill   (United States)

Author keywords

Nucleic acid extraction; Nucleic acid purification; PCR inhibition; QPCR; RT qPCR

Indexed keywords

ARCHAEAL DNA; BACTERIAL DNA; DNA; PHENOL; RNA; RNA 16S;

DNA; GENE EXPRESSION; GENETIC ANALYSIS; INHIBITION; MARINE SEDIMENT; NUCLEIC ACID; ORGANIC MATTER; POLYMERASE CHAIN REACTION; RNA;

ARTICLE; COMPARATIVE STUDY; GENETICS; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA GENE; SEDIMENT;

DNA; DNA, ARCHAEAL; DNA, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; GENES, RRNA; GEOLOGIC SEDIMENTS; PHENOL; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, RIBOSOMAL, 16S;

ARCHAEA;

EID: 77949285115     PISSN: 01686496     EISSN: 15746941     Source Type: Journal    
DOI: 10.1111/j.1574-6941.2009.00827.x     Document Type: Article

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