The cell line NCl-H441 is a useful in vitro model for transport studies of human distal lung epithelial barrier

Molecular Pharmaceutics

Volumn 11, Issue 3, 2014, Pages 995-1006

  Salomon, Johanna J ^a   Muchitsch, Viktoria E ^a   Gausterer, Julia C ^a   Schwagerus, Elena ^a   Huwer, Hanno ^b   Daum, Nicole ^c   Lehr, Claus Michael ^c   Ehrhardt, Carsten ^a  

^a TRINITY COLLEGE DUBLIN   (Ireland)

^b Völklingen Heart Centre   (Germany)

^c SAARLAND UNIVERSITY   (Germany)

Author keywords

absorption; drug transporters; inhalation biopharmaceutics; organic cation transporters; P glycoprotein; pulmonary drug disposition

Indexed keywords

4 [4 (DIMETHYLAMINO)STYTYL] N METHYLPYRIDINIUM IODIDE; AMANTADINE; CARNITINE; CARNITINE TRANSPORTER 1; CARNITINE TRANSPORTER 2; DYE; FLUORESCEIN ISOTHIOCYANATE; MULTIDRUG RESISTANCE PROTEIN; MULTIDRUG RESISTANCE PROTEIN 1; ORGANIC CATION TRANSPORTER; ORGANIC CATION TRANSPORTER 1; ORGANIC CATION TRANSPORTER 2; ORGANIC CATION TRANSPORTER 3; PROTEIN ZO1; PYRIDINIUM DERIVATIVE; QUINIDINE; RHODAMINE 123; THIONEINE; UNCLASSIFIED DRUG; UVOMORULIN; VERAPAMIL; ZOSUQUIDAR;

ARTICLE; CARCINOMA CELL; CELL LINE; CELL STRAIN NCL H441; CONTROLLED STUDY; DRUG DISPOSITION; DRUG TRANSPORT; DRUG UPTAKE; ELECTRIC RESISTANCE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; INHALATION; LUNG ADENOCARCINOMA; LUNG ALVEOLUS EPITHELIUM; MOLECULAR WEIGHT; MONOLAYER CULTURE; PERMEABILITY BARRIER; PRIMARY CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING; ADENOCARCINOMA; BIOLOGICAL MODEL; CELL CULTURE; CYTOLOGY; ENZYME IMMUNOASSAY; EPITHELIUM CELL; LUNG; LUNG ALVEOLUS; LUNG TUMOR; METABOLISM; PATHOLOGY; TIGHT JUNCTION; TRANSPORT AT THE CELLULAR LEVEL;

ADENOCARCINOMA; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CELLS, CULTURED; EPITHELIAL CELLS; HUMANS; IMMUNOENZYME TECHNIQUES; IN VITRO TECHNIQUES; LUNG; LUNG NEOPLASMS; MODELS, BIOLOGICAL; ORGANIC CATION TRANSPORT PROTEINS; P-GLYCOPROTEINS; PULMONARY ALVEOLI; RHODAMINE 123; TIGHT JUNCTIONS;

EID: 84901743670     PISSN: 15438384     EISSN: 15438392     Source Type: Journal    
DOI: 10.1021/mp4006535     Document Type: Article

References (51)

1. Patton, J. S.; Byron, P. R. Inhaling medicines: delivering drugs to the body through the lungs Nat. Rev. Drug Discovery 2007, 6, 67-74
2. Patton, J. S.; Brain, J. D.; Davies, L. A.; Fiegel, J.; Gumbleton, M.; Kim, K. J.; Sakagami, M.; Vanbever, R.; Ehrhardt, C. The particle has landed-discerning the fate of inhaled pharmaceuticals J. Aerosol Med. Pulm. Drug Delivery 2010, 23, S71-87
3. Kim, K. J.; Borok, Z.; Crandall, E. D. A useful in vitro model for transport studies of alveolar epithelial barrier Pharm. Res. 2001, 18, 253-5
4. Forbes, B.; Ehrhardt, C. Human respiratory epithelial cell culture for drug delivery applications Eur. J. Pharm. Biopharm. 2005, 60 (2) 193-205
5. Ehrhardt, C.; Forbes, B.; Kim, K. J. In vitro models of the Tracheo-Bronchial Epithelium. In Drug Absorption Studies. In Situ, In Vitro and In Silico Models; Ehrhardt, C.; Kim, K. J., Eds.; Springer, AAPS Press: New York, 2008; pp 235-257.
6. Benediktsdóttir, B. E.; Arason, A. J.; Halldórsson, S.; Gudjónsson, T.; Másson, M.; Baldursson, O. Drug delivery characteristics of the progenitor bronchial epithelial cell line VA10 Pharm. Res. 2013, 30 (3) 781-91
7. Weibel, E. R. Morphometry of the Human Lung; Springer: Heidelberg, 1963.
8. Stone, K. C.; Mercer, R. R.; Gehr, P.; Stockstill, B.; Crapo, J. D. Allometric relationships of cell numbers and size in the mammalian lung Am. J. Respir. Cell Mol. Biol. 1992, 6 (2) 235-43
9. Kawada, H.; Horiuchi, T.; Shannon, J. M.; Kuroki, Y.; Voelker, D. R.; Mason, R. J. Alveolar type II cells, surfactant protein A (SP-A), and the phospholipid components of surfactant in acute silicosis in the rat Am. Rev. Respir. Dis. 1989, 140 (2) 460-70
10. Williams, M. C. Alveolar type I cells: molecular phenotype and development Annu. Rev. Physiol. 2003, 65, 669-95
11. Ehrhardt, C.; Laue, M.; Kim, K. J. In Vitro Models of the Alveolar Epithelial Barrier. In Drug Absorption Studies. In Situ, In Vitro and In Silico Models; Ehrhardt, C.; Kim, K. J., Eds.; Springer, AAPS Press: New York, 2008; pp 258-282.
12. Li, C.; Wainhaus, S.; Uss, A. S.; Cheng, K. High-Throughput Screening Using Caco-2 cell and PAMPA Systems. In Drug Absorption Studies. In Situ, In Vitro and In Silico Models. Ehrhardt, C.; Kim, K. J., Eds.; Springer, AAPS Press: New York, 2008; pp 418-429.
13. Lieber, M.; Smith, B.; Szakal, A.; Nelson-Rees, W.; Todaro, G. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells Int. J. Cancer 1976, 17 (1) 62-70
14. Sporty, J. L.; Horálková, L.; Ehrhardt, C. In vitro cell culture models for the assessment of pulmonary drug disposition Expert Opin. Drug Metab. Toxicol. 2008, 4, 333-45
15. Elbert, K. J.; Schäfer, U. F.; Schäfers, H. J.; Kim, K. J.; Lee, V. H.; Lehr, C. M. Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies Pharm. Res. 1999, 16 (5) 601-8
16. Foster, K. A.; Oster, C. G.; Mayer, M. M.; Avery, M. L.; Audus, K. L. Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism Exp. Cell Res. 1998, 243 (2) 359-66
17. van den Bogaard, E. H.; Dailey, L. A.; Thorley, A. J.; Tetley, T. D.; Forbes, B. Inflammatory response and barrier properties of a new alveolar type 1-like cell line (TT1) Pharm. Res. 2009, 26 (5) 1172-80
18. Hermanns, M. I.; Unger, R. E.; Kehe, K.; Peters, K.; Kirkpatrick, C. J. Lung epithelial cell lines in coculture with human pulmonary microvascular endothelial cells: development of an alveolo-capillary barrier in vitro Lab. Invest. 2004, 84 (6) 736-52
19. Vuong, H.; Patterson, T.; Adiseshaiah, P.; Shapiro, P.; Kalvakolanu, D. V.; Reddy, S. P. JNK1 and AP-1 regulate PMA-inducible squamous differentiation marker expression in Clara-like H441 cells Am. J. Physiol. Lung Cell Mol. Physiol. 2002, 282 (2) L215-25
20. Gazdar, A. F.; Linnoila, R. I.; Kurita, Y.; Oie, H. K.; Mulshine, J. L.; Clark, J. C.; Whitsett, J. A. Peripheral airway cell differentiation in human lung cancer cell lines Cancer Res. 1990, 50 (17) 5481-7
21. Neuhaus, W.; Samwer, F.; Kunzmann, S.; Muellenbach, R. M.; Wirth, M.; Speer, C. P.; Roewer, N.; Förster, C. Y. Lung endothelial cells strengthen, but brain endothelial cells weaken barrier properties of a human alveolar epithelium cell culture model Differentiation 2012, 84 (4) 294-304
22. Endter, S.; Becker, U.; Daum, N.; Huwer, H.; Lehr, C. M.; Gumbleton, M.; Ehrhardt, C. P-glycoprotein (MDR1) functional activity in human alveolar epithelial cell monolayers Cell Tissue Res. 2007, 328 (1) 77-84
23. Salomon, J. J.; Ehrhardt, C. Organic cation transporters in the blood-air barrier: expression and implications for pulmonary drug delivery Ther. Delivery 2012, 3 (6) 735-47
24. Ehrhardt, C.; Kim, K. J.; Lehr, C. M. Isolation and culture of human alveolar epithelial cells Methods Mol. Med. 2005, 107, 207-6
25. Demling, N.; Ehrhardt, C.; Kasper, M.; Laue, M.; Knels, L.; Rieber, E. P. Promotion of cell adherence and spreading: a novel function of RAGE, the highly selective differentiation marker of human alveolar epithelial type I cells Cell Tissue Res. 2006, 323 (3) 475-88
26. Ehrhardt, C.; Fiegel, J.; Fuchs, S.; Abu-Dahab, R.; Schaefer, U. F.; Hanes, J.; Lehr, C. M. Drug absorption by the respiratory mucosa: cell culture models and particulate drug carriers J. Aerosol Med. 2002, 15 (2) 131-9
27. +) in human respiratory epithelial cells Eur. J. Pharm. Biopharm. 2012, 81 (2) 351-9
28. Koepsell, H.; Lips, K.; Volk, C. Polyspecific organic cation transporters: structure, function, physiological roles, and biopharmaceutical implications Pharm. Res. 2007, 24, 1227-51
29. Nies, A. T.; Koepsell, H.; Damme, K.; Schwab, M. Organic cation transporters (OCTs, MATEs), in vitro and in vivo evidence for the importance in drug therapy Handb. Exp. Pharmacol. 2011, 201, 105-67
30. Cheek, J. M.; Evans, M. J.; Crandall, E. D. Type I cell-like morphology in tight alveolar epithelial monolayers Exp. Cell Res. 1989, 184 (2) 375-87
31. Peixoto, E. B.; Collares-Buzato, C. B. Modulation of the epithelial barrier by dexamethasone and prolactin in cultured Madin-Darby canine kidney (MDCK) cells Cell Biol. Int. 2006, 30 (2) 101-13
32. Ladenburger, A.; Seehase, M.; Kramer, B. W.; Thomas, W.; Wirbelauer, J.; Speer, C. P.; Kunzmann, S. Glucocorticoids potentiate IL-6-induced SP-B expression in H441 cells by enhancing the JAK-STAT signaling pathway Am. J. Physiol. Lung Cell. Mol. Physiol. 2010, 299 (4) L578-84
33. Stentebjerg-Andersen, A.; Notlevsen, I. V.; Brodin, B.; Nielsen, C. U. Calu-3 cells grown under AIC and LCC conditions: implications for dipeptide uptake and transepithelial transport of substances Eur. J. Pharm. Biopharm. 2003, 78 (1) 19-26
34. Forbes, B.; Shah, A.; Martin, G. P.; Lansley, A. B. The human bronchial epithelial cell line 16HBE14o-as a model system of the airways for studying drug transport Int. J. Pharm. 2003, 257 (1-2) 161-7
35. Ehrhardt, C.; Kneuer, C.; Fiegel, J.; Hanes, J.; Schaefer, U. F.; Kim, K. J.; Lehr, C. M. Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o-: implications for the use of this cell line as an in vitro model for bronchial drug absorption studies Cell Tissue Res. 2002, 308 (3) 391-400
36. Ehrhardt, C.; Collnot, E. M.; Baldes, C.; Becker, U.; Laue, M.; Kim, K. J.; Lehr, C. M. Towards an in vitro model of cystic fibrosis small airway epithelium: characterisation of the human bronchial epithelial cell line CFBE41o-Cell Tissue Res. 2006, 323 (3) 405-15
37. Grainger, C. I.; Greenwell, L. L.; Lockley, D. J.; Martin, G. P.; Forbes, B. Culture of Calu-3 cells at the air interface provides a representative model of the airway epithelial barrier Pharm. Res. 2006, 23 (7) 1482-90
38. Tronde, A.; Nordén, B.; Jeppsson, A. B.; Brunmark, P.; Nilsson, E.; Lennernäs, H.; Bengtsson, U. H. Drug absorption from the isolated perfused rat lung-correlations with drug physicochemical properties and epithelial permeability J. Drug Target 2003, 11 (1) 61-74
39. Gumbleton, M.; Al-Jayyoussi, G.; Crandon-Lewis, A.; Francombe, D.; Kreitmeyr, K.; Morris, C. J.; Smith, M. W. Spatial expression and functionality of drug transporters in the intact lung: Objectives for further research Adv. Drug Delivery Rev. 2011, 63 (1-2) 110-8
40. Endter, S.; Francombe, D.; Ehrhardt, C.; Gumbleton, M. RT-PCR analysis of ABC, SLC and SLCO drug transporters in human lung epithelial cell models J. Pharm. Pharmacol. 2009, 61, 583-91
41. Campbell, L.; Abulrob, A. N.; Kandalaft, L. E.; Plummer, S.; Hollins, A. J.; Gibbs, A.; Gumbleton, M. Constitutive expression of p-glycoprotein in normal lung alveolar epithelium and functionality in primary alveolar epithelial cultures J. Pharmacol. Exp. Ther. 2003, 304 (1) 441-52
42. Ehrhardt, C.; Kneuer, C.; Laue, M.; Schaefer, U. F.; Kim, K. J.; Lehr, C. M. 16HBE14o-human bronchial epithelial cell layers express P-glycoprotein, lung resistance-related protein, and caveolin-1 Pharm. Res. 2003, 20 (4) 545-51
43. Madlova, M.; Bosquillon, C.; Asker, D.; Dolezal, P.; Forbes, B. In-vitro respiratory drug absorption models possess nominal functional P-glycoprotein activity J. Pharm. Pharmacol. 2009, 61 (3) 293-301
44. Cormet-Boyaka, E.; Huneau, J. F.; Mordrelle, A.; Boyaka, P. N.; Carbon, C.; Rubinstein, E.; Tomé, D. Secretion of sparfloxacin from the human intestinal Caco-2 cell line is altered by P-glycoprotein inhibitors Antimicrob. Agents Chemother. 1998, 42 (10) 2607-11
45. Haghi, M.; Young, P. M.; Traini, D.; Jaiswal, R.; Gong, J.; Bebawy, M. Time-and passage-dependent characteristics of a Calu-3 respiratory epithelial cell model Drug Dev. Ind. Pharm. 2010, 36 (10) 1207-14
46. Troutman, M. D.; Thakker, D. R. Efflux ratio cannot assess P-glycoprotein-mediated attenuation of absorptive transport: asymmetric effect of P-glycoprotein on absorptive and secretory transport across Caco-2 cell monolayers Pharm. Res. 2003, 20 (8) 1200-9
47. Ehrhardt, C.; Kneuer, C.; Bies, C.; Lehr, C. M.; Kim, K. J.; Bakowsky, U. Salbutamol is actively absorbed across human bronchial epithelial cell layers Pulm. Pharmacol. Ther. 2005, 18 (3) 165-70
48. Lips, K. S.; Volk, C.; Schmitt, B. M.; Pfeil, U.; Arndt, P.; Miska, D.; Ermert, L.; Kummer, W.; Koepsell, H. Polyspecific cation transporters mediate luminal release of acetylcholine from bronchial epithelium Am. J. Respir. Cell Mol. Biol. 2005, 33, 79-88
49. Horvath, G.; Schmid, N.; Fragoso, M. A.; Schmid, A.; Conner, G. E.; Salathe, M.; Wanner, E. Epithelial organic cation transporters ensure pH-dependent drug absorption in the airway Am. J. Respir. Cell Mol. Biol. 2007, 36, 53-60
50. Mukherjee, M.; Pritchard, D. I.; Bosquillon, C. Evaluation of air-interfaced Calu-3 cell layers for investigation of inhaled drug interactions with organic cation transporters in vitro Int. J. Pharm. 2012, 426 (1-2) 7-14
51. Grigat, S.; Fork, C.; Bach, M.; Golz, S.; Geerts, A.; Schömig, E.; Gründemann, D. The carnitine transporter SLC22A5 is not a general drug transporter, but it efficiently translocates mildronate Drug Metab. Dispos. 2009, 37 (2) 330-7