Time- and passage-dependent characteristics of a Calu-3 respiratory epithelial cell model

Drug Development and Industrial Pharmacy

Volumn 36, Issue 10, 2010, Pages 1207-1214

  Haghi, Mehra ^a   Young, Paul M ^a   Traini, Daniela ^a   Jaiswal, Ritu ^a   Gong, Joyce ^a   Bebawy, Mary ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Calu 3; Culture conditions; P glycoprotein; P gp; Respiratory epithelium

Indexed keywords

GLYCOPROTEIN P;

ARTICLE; CALU 3 CELL LINE; CELL LINE; CELL MEMBRANE PERMEABILITY; MEASUREMENT; PROTEIN EXPRESSION; PROTEIN FUNCTION; RESPIRATORY EPITHELIUM; TRANSEPITHELIAL ELECTRICAL RESISTANCE;

BIOLOGICAL TRANSPORT; BRONCHI; CELL LINE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; DRUG DISCOVERY; ELECTRIC IMPEDANCE; EPITHELIAL CELLS; HUMANS; MODELS, BIOLOGICAL; P-GLYCOPROTEIN; PHARMACEUTICAL PREPARATIONS; RESPIRATORY MUCOSA; RHODAMINE 123; TIME FACTORS;

EID: 77956419131     PISSN: 03639045     EISSN: 15205762     Source Type: Journal    
DOI: 10.3109/03639041003695113     Document Type: Article

References (31)

1. Gonda I. (2000). The ascent of pulmonary drug delivery. J Pharm Sci, 89:940-5.
2. Gonda I. (2006). Systemic delivery of drugs to humans via inhalation. J Aerosol Med, 19:47-53.
3. Sullivan VJ, Mikszta JA, Laurent P, Huang J, Ford B. (2006). Noninvasive delivery technologies: Respiratory delivery of vaccines. Expert Opin Drug Deliv, 3:87-95.
4. Elbert KJ, Schäfer UF, Schäfers H-J, Kim, K-J, Lee VHL, Lehr CM. (1999). Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies. Pharm Res, 16:601-8.
5. Forbes B, Shah A, Martin GP, Lansley AB, Forbes B, Shah A, et al. (2003). The human bronchial epithelial cell line 16HBE14o- as a model system of the airways for studying drug transport. Int J Pharm, 257:161-7.
6. Mathias NR, Timoszyk J, Stetsko PI, Megill JR, Smith RL, Wall DA, et al. (2002). Permeability characteristics of Calu-3 human bronchial epithelial cells: In vitro-in vivo correlation to predict lung absorption in rats. J Drug Target, 10:31-40.
7. Forbes B, Ehrhardt C, Forbes B, Ehrhardt C. (2005). Human respiratory epithelial cell culture for drug delivery applications. Eur J Pharm Biopharm, 60:193-205.
8. Foster KA, Avery ML, Yazdanian M, Audus KL. (2000). Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery. Int J Pharm, 208:1-11.
9. Hamilton KO, Topp E, Makagiansar I, Siahaan T, Yazdanian M, Audus KL. (2001). Multidrug resistance-associated protein-1 functional activity in Calu-3 cells. J Pharmacol Exp Ther, 298:1199-205.
10. Ehrhardt C, Fiegel J, Fuchs S, Abu-Dahab R, Schaefer UF, Hanes J, et al. (2002). Drug absorption by the respiratory mucosa: Cell culture models and particulate drug carriers. J Aerosol Med, 15:131-9.
11. Ramesh Babu PB, Chidekel A, Utidjian L, Shaffer TH. (2004). Regulation of apical surface fluid and protein secretion in human airway epithelial cell line Calu-3. Biochem Biophys Res Commun, 319:1132-7. (Pubitemid 38759529)
12. Grainger CI, Greenwell LL, Lockley DJ, Martin GP, Forbes B. (2006). Culture of Calu-3 cells at the air interface provides a representative model of the airway epithelial barrier. Pharm Res, 23:1482-90.
13. Bebawy M, Morris MB, Roufogalis BD. (2001). Selective modulation of P-glycoprotein-mediated drug resistance. Br J Cancer, 85:1998-2003.
14. Fenneteau F, Turgeon J, Couture L, Michaud V, Li J, Nekka F. (2009). Assessing drug distribution in tissues expressing Pglycoprotein through physiologically based pharmacokinetic modeling: Model structure and parameters determination. Theor Biol Med Model, 6:2.
15. Fromm MF. (2003). Importance of P-glycoprotein for drug disposition in humans. Eur J Clin Invest, 33:6-9.
16. Madlova M, Bosquillon C, Asker D, Dolezal P, Forbes B. (2009). In-vitro respiratory drug absorption models possess nominal functional P-glycoprotein activity. J Pharm Pharmacol, 61:293-301.
17. Florea BI, Cassara ML, Junginger HE, Borchard G, Florea BI, Cassara ML, et al. (2003). Drug transport and metabolism characteristics of the human airway epithelial cell line Calu-3. J Control Release, 87:131-8.
18. Borchard G, Cassara ML, Roemele PE, Florea BI, Junginger HE, Borchard G, et al. (2002). Transport and local metabolism of budesonide and fluticasone propionate in a human bronchial epithelial cell line (Calu-3). J Pharm Sci, 91:1561-7.
19. Fiegel J, Ehrhardt C, Schaefer UF, Lehr CM, Hanes J, Fiegel J, et al. (2003). Large porous particle impingement on lung epithelial cell monolayers-toward improved particle characterization in the lung. Pharm Res, 20:788-96.
20. Inglis SK, Corboz MR, Ballard ST. (1998). Effect of anion secretion inhibitors on mucin content of airway submucosal gland ducts. Am J Physiol Lung Cell Mol Physiol, 274:L762-6.
21. Hamilton KO, Backstrom G, Yazdanian MA, Audus KL (2001) P-glycoprotein efflux pump expression and activity in Calu-3 cells. J Pharm Sci, 90:647-58.
22. Behrens I, Kissel T. (2003). Do cell culture conditions influence the carrier-mediated transport of peptides in Caco-2 cell monolayers? Eur J Pharm Sci, 19:433-42.
23. Butler M, Thilly W. (1982). MDCK microcarrier cultures: Seeding density effects and amino acid utilization. In Vitro Cell Dev Biol Plant, 18:213-19.
24. Chen X-M, Kitts DD. (2008). Determining conditions for nitric oxide synthesis in Caco-2 cells using taguchi and factorial experimental designs. Anal Biochem, 381:185-92.
25. Ng Y, Chase H. (2008). Novel bioreactors for the culture and expansion of aggregative neural stem cells. Bioprocess Biosyst Eng, 31:393-400.
26. Grobstein C. (1953). Morphogenetic interaction between embryonic mouse tissues separated by a membrane filter. Nature, 172:869-71.
27. Toropainen E, Ranta V-P, Talvitie A, Suhonen P, Urtti A. (2001). Culture model of human corneal epithelium for prediction of ocular drug absorption. Invest Ophthalmol Vis Sci, 42:2942-8.
28. Turowski P, Adamson P, Sathia J, Zhang JJ, Moss SE, Aylward GW, et al. (2004). Basement membrane-dependent modification of phenotype and gene expression in human retinal pigment epithelial ARPE-19 cells. Invest Ophthalmol Vis Sci, 45:2786-94.
29. Widdicombe J, Sachs L, Finkbeiner W. (2003). Effects of growth surface on differentiation of cultures of human tracheal epithelium. In Vitro Cell Dev Biol Animal, 39:51-5. (Pubitemid 36944609)
30. Fu D, Bebawy M, Kable EP, Roufogalis BD, Fu D, Bebawy M, et al. (2004). Dynamic and intracellular trafficking of P-glycoprotein- EGFP fusion protein: Implications in multidrug resistance in cancer. Int J Cancer, 109:174-81.
31. Crivellato E, Travan L, Candussio L, Klugmann F, Decorti G. (1997). Identification of P-glycoprotein at the membrane of mast cell secretory granules. An immunofluorescence and protein Agold electron microscopical investigation. Histochem J, 29:193-8.