Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies

Pharmaceutical Research

Volumn 16, Issue 5, 1999, Pages 601-608

  Elbert, Katharina J ^a   Schäfer, Ulrich F ^a   Schäfers, Hans Joachim ^b   Kim, Kwang Jin ^c   Lee, Vincent H L ^d   Lehr, Claus Michael ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

^c OMC Clinical Research Center   (United States)

^d UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

Drug transport; Histochemical characterization; Human alveolar epithelium; Lectin binding; Primary cell culture

Indexed keywords

ARTICLE; CELL CULTURE; DRUG ABSORPTION; DRUG UPTAKE; EPITHELIUM CELL; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; LECTIN BINDING; LUNG ALVEOLUS CELL TYPE 2; LUNG ALVEOLUS EPITHELIUM; PRIORITY JOURNAL;

ABSORPTION; ADMINISTRATION, INHALATION; BIOLOGICAL TRANSPORT; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; DEXTRANS; DRUG DELIVERY SYSTEMS; ELECTRIC CONDUCTIVITY; ELECTRIC IMPEDANCE; EPITHELIAL CELLS; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; LECTINS; MEMBRANES, ARTIFICIAL; PULMONARY ALVEOLI;

EID: 0344299169     PISSN: 07248741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018887501927     Document Type: Article

References (32)

1. 1. A. E. Taylor and K. A. Gaar. Estimation of equivalent pore radii of pulmonary capillary and alveolar membranes. Am. J. Physiol. 218:1133-1140 (1970).
2. 2. I. C. S. Normand, R. E. Olver, E. O. R. Reynolds, and L. B. Strang. Permeability of lung capillaries and alveoli to non-electrolytes in the foetal lamb. J. Physiol. 219:303-330 (1971).
3. 3. I. Y. R. Adamson and D. H. Bowden. The type 2 cell as a progenitor of alveolar epithelial regeneration. Lab. Invest. 30:35-42 (1974).
4. 2. Exp. Mol. Pathol. 22:142-150 (1975).
5. 5. J. D. Crapo, B. E. Barry, P. Gehr, M. Bachofen, and E. R. Weibel. Cell number and cell characteristics of the normal human lung. Am. Rev. Respir. Dis. 125:332-337 (1982).
6. 6. R. J. Mason and M. C. Williams. Phospholipid composition and ultrastructure of A549 cells and other cultured pulmonary epithelial cells of presumed type II cell origin. Biochim. Biophys. Acta 617:36-50 (1980).
7. 7. P.-G. Germann, S. Ueberschär, A. Gerull, and M. Emura. In vitro induction of type II pneumocyte-related differentiation in a clonal fetal bronchiolo-alveolar epithelial cell line (M3E3/C3). Exp. Toxic. Pathol. 45:315-324 (1993).
8. 8. A. Masuda, M. Kondo, T. Saito, Y. Yatabe, T. Kobayashi, M. Okamoto, M. Suyama, T. Takahashi, and T. Takahashi. Establishment of human peripheral lung epithelial cell lines (HPL1) retaining differentiated characteristics and responsiveness to epidermal growth factor, hepatocyte growth factor, and transforming growth factor beta1 1. Cancer Res. 57:4898-4904 (1997).
9. 9. J. M. Cheek, K. J. Kim, and E. D. Crandall. Tight monolayers of rat alveolar epithelial cells: bioelectric properties and active sodium transport. Am. J. Physiol. 256:C688-C693 (1989).
10. 10. K. J. Kim, D. J. Suh, R. L. Lubman, S. I. Danto, Z. Borok, and E. D. Crandall. Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers. J. Tiss. Cult. Meth. 14:187-194 (1992).
11. 11. Y. Matsukawa, V. H. L. Lee, E. D. Crandall, and K. J. Kim. Size-dependent dextran transport across rat alveolar epithelial cell monolayers. J. Pharm. Sci. 86:305-309 (1997).
12. 12. J. Shen, K. J. Elbert, C.-M. Lehr, F. Yamashita, K. J. Kim, and V. H. L. Lee. Cultured rabbit alveolar epithelial barrier for organic cation transport studies. Pharm. Res. 14:S-134 (1997).
13. 13. P. C. Robinson, D. R. Voelker, and R. J. Mason. Isolation and culture of human alveolar type II epithelial cells. Am. Rev. Respir. Dis. 130:1156-1160 (1984).
14. 14. P. L. Ballard, R. Ertsey, L. K. Gonzales, H. G. Liley, and M. C. Williams. Isolation and characterization of differentiated alveolar type II cells from fetal human lung. Biochim. Biophys. Acta 883:335-344 (1986).
15. 15. D. L. Shapiro, J. N. Finkelstein, and T. van Diver. Isolation of alveolar epithelial cells from lung tissue obtained at autopsy. In Vitro Cell. Dev. Biol. 25:1051-1054 (1989).
16. 16. T. R. Devereux, T. E. Massey, M. R. van Scott, J. Yankaskas, and J. R. Fouts. Xenobiotic metabolism in human alveolar type II cells isolated by centrifugal elutriation and density gradient centrifugation. Cancer Res. 46:5438-5443 (1986).
17. 17. T. Papadopoulos, L. Ionescu, J. Dämmrich, H. Toomes, and H. K. Müller-Hermelink. Type I and type IV collagens promote adherence and spreading of human type II pneumocytes in vitro. Lab. Invest. 62:562-569 (1990).
18. 18. L. Bingle, T. B. Bull, B. Fox, A. Guz, R. J. Richards, and T. D. Tetley. Type II pneumocytes in mixed cell culture of human lung: a light and electron microscopic study. Environ. Health Perspect. 85:71-80 (1990).
19. 19. F. J. van Overveld, P. G. Jorens, W. A. De Backer, M. Rampart, L. Bossaert, and P. A. Vermeire. Release of arachidonic acid metabolites from isolated human alveolar type II cells. Prostaglandins 44:101-110 (1992).
20. 20. P. H. M. Hoet, C. P. L. Lewis, M. Demedts, and B. Nemery. Putrescine and paraquat uptake in human lung slices and isolated type II pneumocytes. Biochem. Pharmacol. 48:517-524 (1994).
21. 21. A. C. Cunningham, D. S. Milne, J. Wilkes, J. H. Dark, T. D. Tetley, and J. A. Kirby. Constitutive expression of MHC and adhesion molecules by alveolar epithelial cells (type II pneumocytes) isolated from human lung and comparison with immunocytochemical findings. J. Cell. Sci. 107:443-449 (1994).
22. 22. J. F. Lechner and M. A. LaVeck. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tiss. Cult. Meth. 9:43-48 (1985).
23. 23. J. D. Edelson, J. M. Shannon, and R. J. Mason. Alkaline phosphatase: A marker of alveolar type II cell differentiation. Am. Rev. Respir. Dis. 138:1268-1275 (1988).
24. 24. L. D. Leder and R. Nicolas. Long-term investigations of the cytologic and enzyme cytochemical behaviour of skin window macrophages. Klin. Wochenschr. 43:684-690 (1965).
25. 25. L. G. Dobbs, M. C. Williams, and A. E. Brandt. Changes in biochemical characteristics and pattern of lectin binding of alveolar type II cells with time in culture. Biochim. Biophys. Acta 846:155-166 (1985).
26. 26. P. W. Bankston, G. A. Porter, A. J. Milici, and G. E. Palade. Differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by Lycopersicon esculentum and Griffonia simplicifolia I lectins. Eur. J. Cell Biol. 54:187-195 (1991).
27. 27. M. Kasper, T. Rudolf, R. Hahn, I. Peterson, and M. Müller. Immuno-and lectin histochemistry of epithelial subtypes and their changes in a radiation-induced lung fibrosis model of the mini pig. Histochemistry 100:367-377 (1993).
28. 28. J. Theodore, E. D. Robin, R. Gaudio, and J. Acevedo. Transalveolar transport of large polar solutes (sucrose, inulin, and dextran). Am. J. Physiol. 229:989-996 (1975).
29. 29. S. J. Enna and L. S. Schanker. Absorption of drugs from the rat lung. Am. J. Physiol. 223:1227-1231 (1972).
30. 30. G. Taylor. The absorption and metabolism of xenobiotics in the lung. Adv. Drug Deliv. Rev. 5:37-61 (1990).
31. 31. T. Morita, A. Yamamoto, M. Hashida, and H. Sezaki. Effects of various absorption promotors on pulmonary absorption of drugs with different molecular weights. Biol. Pharm. Bull. 16:259-262 (1993).
32. 32. S. Kobayashi, S. Kondo, and K. Juni. Permeability of peptides and proteins in human cultured alveolar A549 cell monolayer. Pharm. Res. 12:1115-1119 (1995).