P-glycoprotein (MDR1) functional activity in human alveolar epithelial cell monolayers

Cell and Tissue Research

Volumn 328, Issue 1, 2007, Pages 77-84

  Endter, Sibylle ^a,b   Becker, Ulrich ^c   Daum, Nicole ^c   Huwer, Hanno ^d   Lehr, Claus Michael ^c   Gumbleton, Mark ^b   Ehrhardt, Carsten ^a,c  

^a TRINITY COLLEGE DUBLIN   (Ireland)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c SAARLAND UNIVERSITY   (Germany)

^d Volklingen Heart Centre   (Germany)

Author keywords

ABC transporters; Alveolar epithelial cells; Alveolar epithelium; Cell culture; Human; Multi drug resistance; Pulmonary drug delivery

Indexed keywords

FLUORESCEIN SODIUM; MESSENGER RNA; MULTIDRUG RESISTANCE PROTEIN 1; RHODAMINE 123; VERAPAMIL;

ARTICLE; CELL ISOLATION; CELL STRUCTURE; CONTROLLED STUDY; DRUG EFFECT; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LUNG ALVEOLUS CELL; LUNG PARENCHYMA; MODEL; MOLECULAR BIOLOGY; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSPORT KINETICS; WESTERN BLOTTING;

CELL MEMBRANE PERMEABILITY; CELLS, CULTURED; EPITHELIAL CELLS; GENE EXPRESSION REGULATION; HUMANS; P-GLYCOPROTEIN; PROTEIN TRANSPORT; PULMONARY ALVEOLI; RHODAMINE 123; RNA, MESSENGER;

EID: 33947104217     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00441-006-0346-6     Document Type: Article

References (32)

1. Abulrob AG, Gumbleton M (1999) Transport of phosphatidylcholine in MDR3-negative epithelial cell lines via drug-induced MDR1 P-glycoprotein. Biochem Biophys Res Commun 262:121-126
2. Axiotis CA, Bani D, Bianchi S, Pioli P, Tanini A, Brandi ML (1995) P-glycoprotein is expressed in parathyroid epithelium and is regulated by calcium. Calcif Tissue Int 56:170-174
3. Bosch I, Dunussi-Joannopoulos K,Wu RL, Furlong ST, Croop J (1997) Phosphatidylcholine and phosphatidylethanolamine behave as substrates of the human MDR1 P-glycoprotein. Biochemistry 36:5685-5694
4. Campbell L, Abulrob AN, Kandalaft LE, Plummer S, Hollins AJ, Gibbs A, Gumbleton M (2003) Constitutive expression of P-glycoprotein in normal lung alveolar epithelium and functionality in primary alveolar epithelial cultures. J Pharmacol Exp Ther 304:441-452
5. Chen J, Chen Z, Narasaraju T, Jin N, Liu L (2004) Isolation of highly pure alveolar epithelial type I and type II cells from rat lungs. Lab Invest 84:727-735 (Erratum in: Lab Invest 85:1181)
6. Cordon-Cardo C, O'Brien LP, Boccia J, Casals D, Bertino JR, Melamed MR (1990) Expression of the multidrug resistance gene product (P-glycoprotein) in human normal and tumor tissues. J Histochem Cytochem 38:1277-1287
7. Demeule M, Jodoin J, Gingras D, Beliveau R (2000) P-glycoprotein is localized in caveolae in resistant cells and in brain capillaries. FEBS Lett 466:219-224
8. Demeule M, Shedid D, Beaulieu E, Del Maestro RF, Moghrabi A, Ghosn PB, Moumdjian R, Berthelet F, Beliveau R (2001) Expression of multidrug-resistance P-glycoprotein (MDR1) in human brain tumors. Int J Cancer 93:62-66
9. Demling N, Ehrhardt C, Kasper M, Laue M, Knels L, Rieber EP (2006) Promotion of cell adherence and spreading: a novel function of RAGE, the highly selective differentiation marker of human alveolar epithelial type I cells. Cell Tissue Res 323:475-488
10. Dobbs LG, Gonzales RF (2002) Isolation and culture of pulmonary alveolar epithelial type II cells. In: Freshney RI, Freshney M (eds) Culture of epithelial cells, 2nd edn.Wiley, New York, pp 277-301
11. Ehrhardt C, Kneuer C, Fiegel J, Hanes J, Schaefer UF, Kim KJ, Lehr CM (2002) Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o-: implications for the use of this cell line as an in vitro model for bronchial drug absorption studies. Cell Tissue Res 308:391-400
12. Ehrhardt C, Kim KJ, Lehr CM (2005) Isolation and culture of human alveolar epithelial cells. Methods Mol Med 107:207-216
13. Ehrhardt C, Collnot EM, Baldes C, Becker U, Laue M, Kim KJ, Lehr CM (2006) Towards an in vitro model of cystic fibrosis small airway epithelium: characterisation of the human bronchial epithelial cell line CFBE41o-. Cell Tissue Res 323:405-415
14. Elbert KJ, Schafer UF, Schafers HJ, Kim KJ, Lee VH, Lehr CM (1999) Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies. Pharm Res 16:601-608
15. Florea BI, Cassara ML, Junginger HE, Borchard G (2003) Drug transport and metabolism characteristics of the human airway epithelial cell line Calu-3. J Control Release 87:131-138
16. Forbes B, Ehrhardt C (2005) Human respiratory epithelial cell culture for drug delivery applications. Eur J Pharm Biopharm 60:193-205
17. Foster KA, Oster CG, Mayer MM, Avery ML, Audus KL (1998) Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism. Exp Cell Res 243:359-366
18. Fuchs S, Hollins AJ, Laue M, Schaefer UF, Roemer K, Gumbleton M, Lehr CM (2003) Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C. Cell Tissue Res 311:31-45
19. Greiner B, Eichelbaum M, Fritz P, Kreichgauer HP, Richter O von, Zundler J, Kroemer HK (1999) The role of intestinal P-glycoprotein in the interaction of digoxin and rifampin. J Clin Invest 104:147-153
20. Gruenert DC, Finkbeiner WE, Widdicombe JH (1995) Culture and transformation of human airway epithelial cells. Am J Physiol 268:L347-L360
21. Hamilton KO, Backstrom G, Yazdanian MA, Audus KL (2001) P-glycoprotein efflux pump expression and activity in Calu-3 cells. J Pharm Sci 90:647-658
22. Higgins CF (1995) Volume-activated chloride currents associated with the multidrug resistance P-glycoprotein. J Physiol (Lond) 482:31S-36S
23. Kennedy BG, Mangini NJ (2002) P-glycoprotein expression in human retinal pigment epithelium. Mol Vis 8:422-430
24. Kim KJ, Borok Z, Crandall ED (2001) A useful in vitro model for transport studies of alveolar epithelial barrier. Pharm Res 18:253-255
25. Lechapt-Zalcman E, Hurbain I, Lacave R, Commo F, Urban T, Antoine M, Milleron B, Bernaudin JF (1997) MDR1-Pgp 170 expression in human bronchus. Eur Respir J 10:1837-1843
26. Manford F, Tronde A, Jeppsson AB, Patel N, Johansson F, Forbes B (2005) Drug permeability in 16HBE14o- airway cell layers correlates with absorption from the isolated perfused rat lung. Eur J Pharm Sci 26:414-420
27. Mi Q, Cui B, Silva GL, Lantvit D, Lim E, Chai H, You M, Hollingshead MG, Mayo JG, Kinghorn AD, Pezzuto JM, Pervilleine A (2001) A novel tropane alkaloid that reverses the multidrug-resistance phenotype. Cancer Res 261:4030-4037
28. Patton JS, Fishburn CS, Weers JG (2004) The lungs as a portal of entry for systemic drug delivery. Proc Am Thorac Soc 1:338-344
29. Steimer A, Haltner E, Lehr CM (2005) Cell culture models of the respiratory tract relevant to pulmonary drug delivery. J Aerosol Med 18:137-182
30. Stouch TR, Gudmundsson O (2002) Progress in understanding the structure-activity relationships of P-glycoprotein. Adv Drug Deliv Rev 54:315-328
31. Wichert P von, Seifart C (2005) The lung, an organ for absorption? Respiration 72:552-558
32. Wise J, Lechner JF (2002) Normal human bronchial epithelial cell culture. In: Freshney RI, Freshney M (eds) Culture of epithelial cells, 2nd edn. Wiley, New York, pp 257-276