Analysis of hypoxia-induced metabolic reprogramming

Methods in Enzymology

Volumn 542, Issue , 2014, Pages 425-455

  Yang, Chendong ^a   Jiang, Lei ^a   Zhang, Huafeng ^b   Shimoda, Larissa A ^c   Deberardinis, Ralph J ^a   Semenza, Gregg L ^c  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Glycolytic rate; Intracellular pH; Mitochondrial autophagy; Oxygen consumption; Stable isotope labeling

Indexed keywords

ACETYL COENZYME A; CARBON 13; FATTY ACID; GLUCOSE; GLUCOSE TRANSPORTER; GLUTAMINE; GLYCOLYTIC ENZYME; HYPOXIA INDUCIBLE FACTOR 1; LACTIC ACID; PROTEIN BNIP3; REACTIVE OXYGEN METABOLITE; SODIUM PROTON EXCHANGE PROTEIN 1; CARBON; CULTURE MEDIUM;

AEROBIC METABOLISM; ARTICLE; AUTOPHAGY; CELL CULTURE; CELL HYPOXIA; CELL PH; CITRIC ACID CYCLE; CULTURE MEDIUM; FATTY ACID SYNTHESIS; GLUCOSE METABOLISM; GLUCOSE TRANSPORT; GLYCOLYSIS; HUMAN; HYPOXIC CELL; IMMUNOBLOTTING; MITOCHONDRION; MOLECULAR PROBE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OXYGEN CONSUMPTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; UPREGULATION; ANOXIA; CULTURE TECHNIQUE; GENETIC PROCEDURES; METABOLISM; NEOPLASM; PH;

ANOXIA; AUTOPHAGY; CARBON ISOTOPES; CELL CULTURE TECHNIQUES; CULTURE MEDIA; GLUCOSE; GLYCOLYSIS; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOBLOTTING; LACTIC ACID; MAGNETIC RESONANCE SPECTROSCOPY; MITOCHONDRIA; MOLECULAR PROBE TECHNIQUES; NEOPLASMS; REACTIVE OXYGEN SPECIES;

EID: 84901452684     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-416618-9.00022-4     Document Type: Chapter

References (44)

1. G. Bellot, R. Garcia-Medina, P. Gounon, J. Chiche, D. Roux, and J. Pouyssegur et al. Hypoxia-induced autophagy is mediated through hypoxia-inducible factor induction of BNIP3 and BNIP3L via their BH3 domains Molecular and Cellular Biology 29 10 2009 2570 2581
2. S.L. Budd, R.F. Castilho, and D.G. Nicholls Mitochondrial membrane potential and hydroethidine-monitored superoxide generation in cultured cerebellar granule cells FEBS Letters 415 1 1997 21 24
3. T. Cheng, J. Sudderth, C. Yang, A.R. Mullen, E.S. Jin, and J.M. Mates et al. Pyruvate carboxylase is required for glutamine-independent growth of tumor cells Proceedings of the National Academy of Sciences of the United States of America 108 21 2011 8674 8679
4. L.C. Clark Jr., R. Wolf, D. Granger, and Z. Taylor Continuous recording of blood oxygen tensions by polarography Journal of Applied Physiology 6 3 1953 189 193
5. R.J. DeBerardinis, A. Mancuso, E. Daikhin, I. Nissim, M. Yudkoff, and S. Wehrli et al. Beyond aerobic glycolysis: Transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis Proceedings of the National Academy of Sciences of the United States of America 104 49 2007 19345 19350
6. C. Diepart, B.F. Jordan, and B. Gallez A new EPR oximetry protocol to estimate the tissue oxygen consumption in vivo Radiation Research 172 2 2009 220 225
7. B.L. Ebert, J.D. Firth, and P.J. Ratcliffe Hypoxia and mitochondrial inhibitors regulate expression of glucose transporter-1 via distinct cis-acting sequences The Journal of Biological Chemistry 270 49 1995 29083 29089
8. 13C mass isotopomer distributions for natural stable isotope abundance Journal of Mass Spectrometry 31 3 1996 255 262
9. R. Fukuda, H. Zhang, J. Kim, L. Shimoda, C.V. Dang, and G.L. Semenza HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells Cell 129 1 2007 111 122
10. R.D. Guzy, and P.T. Schumacker Oxygen sensing by mitochondria at complex III: The paradox of increased reactive oxygen species during hypoxia Experimental Physiology 91 5 2006 807 819
11. 13C NMR NMR in Biomedicine 25 11 2012 1286 1294
12. J. Hynes, H.R. Hill, and D.B. Papkovsky The use of a fluorescence-based oxygen uptake assay in the analysis of cytotoxicity Toxicology In Vitro 20 5 2006 785 792
13. 2 homeostasis by hypoxia-inducible factor 1α Genes and Development 12 2 1998 149 162
14. Y. Kabeya, N. Mizushima, A. Yamamoto, S. Oshitani-Okamoto, and Y. Ohsumi et al. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation Journal of Cell Science 117 13 2004 2805 2812
15. J.W. Kim, I. Tchernyshyov, G.L. Semenza, and C.V. Dang HIF-1-mediated expression of pyruvate dehydrogenase kinase: A metabolic switch required for cellular adaptation to hypoxia Cell Metabolism 3 3 2006 177 185
16. D.J. Klionsky, F.C. Abdalla, H. Abeliovich, R.T. Abraham, A. Acevedo-Arozena, and K. Adeli et al. Guidelines for the use and interpretation of assays for monitoring autophagy Autophagy 8 4 2012 445 544
17. B. Levine, and D.J. Klionsky Development by self-digestion: Molecular mechanisms and biological functions of autophagy Developmental Cell 6 4 2004 463 477
18. Y. Liang, C. Buettger, D.K. Berner, and F.M. Matschinsky Chronic effect of fatty acids on insulin release is not through the alteration of glucose metabolism in a pancreatic beta-cell line (beta HC9) Diabetologia 40 9 1997 1018 1027
19. C.W. Lu, S.C. Lin, K.F. Chen, Y.Y. Lai, and S.J. Tsai Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible factor-1 promotes metabolic switch and drug resistance The Journal of Biological Chemistry 283 42 2008 28106 28114
20. N.W. Lutz, Y. Le Fur, J. Chiche, J. Pouyssegur, and P.J. Cozzone Quantitative in vivo characterization of intracellular and extracellular pH profiles in heterogeneous tumors: A novel method enabling multiparametric pH analysis Cancer Research 73 15 2013 4616 4628
21. 13C]glucose in human brain tumors in vivo NMR in Biomedicine 25 11 2012 1234 1244
22. I. Marin-Valencia, S.K. Cho, D. Rakheja, K.J. Hatanpaa, P. Kapur, and T. Mashimo et al. Glucose metabolism via the pentose phosphate pathway, glycolysis and Krebs cycle in an orthotopic mouse model of human brain tumors NMR in Biomedicine 25 10 2012 1177 1186
23. I. Marin-Valencia, C. Yang, T. Mashimo, S. Cho, H. Baek, and X.L. Yang et al. Analysis of tumor metabolism reveals mitochondrial glucose oxidation in genetically diverse human glioblastomas in the mouse brain in vivo Cell Metabolism 15 6 2012 827 837
24. H.R. McLennan, and M. Degli Esposti The contribution of mitochondrial respiratory complexes to the production of reactive oxygen species Journal of Bioenergetics and Biomembranes 32 2 2000 153 162
25. T.W. Molter, S.C. McQuaide, M.R. Holl, D.R. Meldrum, J.M. Dragavon, and J.B. Anderson et al. A new approach for measuring single-cell oxygen consumption rates IEEE Transactions on Automation Science and Engineering 5 1 2008 32 42
26. I. Papandreou, R.A. Cairns, L. Fontana, A.L. Lim, and N.C. Denko HIF-1 mediates adaptation to hypoxia by actively downregulating mitochondrial oxygen consumption Cell Metabolism 3 3 2006 187 197
27. U. Pilatus, E. Aboagye, D. Artemov, N. Mori, E. Ackerstaff, and Z.M. Bhujwalla Real-time measurements of cellular oxygen consumption, pH, and energy metabolism using nuclear magnetic resonance spectroscopy Magnetic Resonance in Medicine 45 5 2001 749 755
28. S. Rey, W. Luo, L.A. Shimoda, and G.L. Semenza Metabolic reprogramming by HIF-1 promotes the survival of bone marrow-derived angiogenic cells in ischemic tissue Blood 117 18 2011 4988 4998
29. 2 + in lymphocytes Journal of Cell Biology 95 1 1982 189 196
30. T.N. Seagroves, H.E. Ryan, H. Lu, B.G. Wouters, M. Knapp, and P. Thibault et al. Transcription factor HIF-1 is a necessary mediator of the Pasteur effect in mammalian cells Molecular and Cellular Biology 21 10 2001 3436 3444
31. G.L. Semenza Regulation of metabolism by hypoxia-inducible factor 1 Cold Spring Harbor Symposium on Quantitative Biology 76 2011 347 353
32. G.L. Semenza, B.-H. Jiang, S.W. Leung, R. Passantino, J.-P. Concordet, and P. Maire et al. Hypoxia response elements in the aldolase A, enolase 1, and lactate dehydrogenase A gene promoters contain essential binding sites for hypoxia-inducible factor 1 The Journal of Biological Chemistry 271 51 1996 32529 32537
33. L.A. Shimoda, M. Fallon, S. Pisarcik, J. Wang, and G.L. Semenza HIF-1 regulates hypoxic induction of NHE1 expression and alkalinization of intracellular pH in pulmonary arterial myocytes American Journal of Physiology - Lung Cellular and Molecular Physiology 291 5 2006 L941 L949
34. T. Simsek, F. Kocabas, J. Zheng, R.J. DeBerardinis, A.I. Mahmoud, and E.N. Olsen et al. The distinct metabolic profile of hematopoietic stem cells reflects their location in a hypoxic niche Cell Stem Cell 7 3 2010 380 390
35. P. Swietach, R.D. Vaughan-Jones, and A.L. Harris Regulation of tumor pH and the role of carbonic anhydrase 9 Cancer Metastasis Reviews 26 2 2007 299 310
36. I. Tanida, N. Minematsu-Ikeguchi, T. Ueno, and E. Kominami Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy Autophagy 1 2 2005 84 91
37. I. Tanida, Y.S. Sou, J. Ezaki, N. Minematsu-Ikeguchi, T. Ueno, and E. Kominami HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates The Journal of Biological Chemistry 279 35 2004 36268 36276
38. T. TeSlaa, and T.A. Teitell Conventional techniques to measure glycolytic flux Methods in Enzymology 542 2014
39. M.S. Ullah, A.J. Davies, and A.P. Halestrap The plasma membrane lactate transporter MCT4, but not MCT1, is up-regulated by hypoxia through a HIF-1α-dependent mechanism The Journal of Biological Chemistry 281 14 2006 9030 9037
40. P. Vaupel, A. Mayer, and M. Hockel Tumor hypoxia and malignant progression Methods in Enzymology 381 2004 335 354
41. 13C isotopic tracers for metabolic flux analysis in mammalian cells Metabolic Engineering 14 2 2012 162 171
42. C. Yang, J. Sudderth, T. Dang, R.M. Bachoo, J.G. McDonald, and R.J. DeBerardinis Glioblastoma cells require glutamate dehydrogenase to survive impairments of glucose metabolism or Akt signaling Cancer Research 69 20 2009 7986 7993
43. N. Zamzami, P. Marchetti, M. Castedo, D. Decaudin, A. Macho, and T. Hirsch et al. Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death Journal of Experimental Medicine 182 2 1995 367 377
44. H. Zhang, M. Bosch-Marce, L.A. Shimoda, Y.S. Tan, J.H. Baek, and J.B. Wesley et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia The Journal of Biological Chemistry 283 16 2008 10892 10903