Evaluation of coumarin-based fluorogenic P450 BM3 substrates and prospects for competitive inhibition screenings

Analytical Biochemistry

Volumn 456, Issue 1, 2014, Pages 70-81

  Neufeld, Katharina ^a   Zu Berstenhorst, Sonja Meyer ^a   Pietruszka, Jörg ^a,b  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

Cytochrome P450 BM3; Fluorescence based assay; High throughput screening; Microtiter plate; P450 inhibition

Indexed keywords

BACTERIOLOGY; FATTY ACIDS; FLUORESCENCE; SUBSTRATES;

CATALYTIC EFFICIENCIES; COMPETITIVE INHIBITION; CYTOCHROME P450 BM3; FLUORESCENT PRODUCTS; FLUOROGENIC SUBSTRATE; HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT ASSAYS; MICROTITER PLATES;

ENZYMES;

7 ALLYLOXYRESORUFIN; 7 ETHOXYCOUMARIN; COUMARIN DERIVATIVE; CYTOCHROME P450 BM3; ETHOXYRESORUFIN; IMIDAZOLE; KETOCONAZOLE; MYRISTIC ACID; NAPHTHALENE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; POTASSIUM CYANIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RESORUFIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CYTOCHROME P450; CYTOCHROME P450 INHIBITOR; FLAVOCYTOCHROME P450 BM3 MONOXYGENASES; FLUORESCENT DYE; OXAZINE DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

ARTICLE; BIOCATALYST; COMPETITIVE INHIBITION; DEALKYLATION; ELECTRON; ENZYME INHIBITION; FLUORESCENCE; HYDROXYLATION; PRIORITY JOURNAL; THIN LAYER CHROMATOGRAPHY; ALKYLATION; ANTAGONISTS AND INHIBITORS; BIOCATALYSIS; CHEMISTRY; DRUG SCREENING; HIGH THROUGHPUT SCREENING; IC50; KINETICS; METABOLISM; PROCEDURES; SYNTHESIS;

ALKYLATION; BACTERIAL PROTEINS; BIOCATALYSIS; COUMARINS; CYTOCHROME P-450 ENZYME INHIBITORS; CYTOCHROME P-450 ENZYME SYSTEM; DRUG EVALUATION, PRECLINICAL; FLUORESCENT DYES; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROXYLATION; INHIBITORY CONCENTRATION 50; KINETICS; NADP; NADPH-FERRIHEMOPROTEIN REDUCTASE; OXAZINES;

EID: 84900565024     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.03.022     Document Type: Article

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