Cost-effective whole-cell assay for laboratory evolution of hydroxylases in Escherichia coli

Journal of Biomolecular Screening

Volumn 6, Issue 2, 2001, Pages 111-117

  Schwaneberg, U ^a   Otey, C ^a   Cirino, P C ^a   Farinas, E ^a   Arnold, F H ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYLIC ACID DERIVATIVE; EDETIC ACID; HEXAMETAPHOSPHATE SODIUM; ISOCITRATE DEHYDROGENASE; ISOCITRIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; POLYETHYLENEIMINE; POLYMYXIN B;

ARTICLE; CELL SUSPENSION; CENTRIFUGATION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CYTOLYSIS; ENZYME ASSAY; ENZYME SYNTHESIS; ESCHERICHIA COLI; MEMBRANE PERMEABILITY; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING TEST;

AUTOMATION; BACILLUS MEGATERIUM; CATALYSIS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; ESCHERICHIA COLI; HYDROXYLATION; ISOCITRATE DEHYDROGENASE; LIGHT; MICROBIAL SENSITIVITY TESTS; MIXED FUNCTION OXYGENASES; NADP; SPECTROPHOTOMETRY; TIME FACTORS; ULTRAVIOLET RAYS;

BACILLUS MEGATERIUM; ESCHERICHIA COLI;

EID: 0035037955     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1089/108705701750160309     Document Type: Article

References (26)

1. P450 superfamily: Update on new sequences, gene mapping, accession numbers and nomenclature
2. Cytochrome P-450 monooxygenase systems in aquatic species: Carcinogen metabolism and biomarkers for carcinogen and pollutant exposure
3. Substrate specificifies and functions of the P450 cytochromes
4. New applications of bacterial systems to problems in toxicology
5. Effects of freezing, thawing, and storage of human liver samples on the microsomal contents and activities of cytochrome P450 enzymes
6. Effects of compatible solutes on mammalian cytochrome P450 stability
7. Directed evolution of biocatalysts
8. Identification and characterization of two functional domains in cytochrome P-450BM-3, a catalytically self-sufficient monooxygenase induced by barbiturates in Bacillus megaterium
9. Coding nucleotide, 5′regulatory, and deduced amino acid sequences of P-450BM-3, a single peptide cytochrome P-450:NADPH-P-450 reductase from Bacillus megaterium
10. In vivo reconstitution of highly active Candida maltosa cytochrome P450 monooxygenase systems in inducible membranes of Saccharomyces cerevisiae
11. The cytochrome P450 4 (CYP4) family
12. Biocatalyst engineering by assembly of fatty acid transport and oxidation activities for in vivo application of cytochrome P-450BM-3 monooxygenase
13. P450 monooxygenase in biotechnology I. Single-step, large-scale purification method for cytochrome P450 BM-3 by anion-exchange chromatography
14. Omega-1, Omega-2, and Omega-3 hydroxylation of long-chain fatty acids, amides and alcohols by a soluble enzyme system from Bacillus megaterium
15. The induction of cytochrome P-450 in the alkane-utilizing yeast Lodderomyces elongisporus
16. Simultaneous radiometric and fluorimetric detection of lauric acid metabolites using high-performance liquid chromatography following esterification with 4-bromomethyl-6,7-dimethoxycoumarin in human and rat liver microsomes
17. The highly stereoselective oxidation of polyunsaturated fatty acids by cytochrome P450BM-3
18. A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A
19. A fully modular vector system for the optimization of gene expression in Escherichia coli
20. The carbon monoaxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature
21. Agents that increase the permeability of the outer membrane
22. Permeabilized cells
23. The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli
24. Pathways of NADPH formation in Escherichia coli
25. +-specific isocitrate dehydrogenase of Escherichia coli. I. Purification and characterization
26. NADP-specific isocitrate dehydrogenase of Escherichia coli. IV. Purification by chromatography on Affi-Gel Blue