A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A

Analytical Biochemistry

Volumn 269, Issue 2, 1999, Pages 359-366

  Schwaneberg, Ulrich ^a   Schmidt Dannert, Claudia ^a   Schmitt, Jutta ^a   Schmid, Rolf Dieter ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOLOGY; CHROMOPHORES; ENZYME ACTIVITY; ESCHERICHIA COLI; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY;

ACTIVITY ASSAYS; BACILLUS MEGATERIUM; CYTOCHROME P450 BM3; ESCHERICHIA COLI CELLS; LONG CHAIN FATTY ACID; MICROTITER PLATE READER; RECOMBINANT ENZYMES; SPECTROPHOTOMETRIC ASSAYS;

FATTY ACIDS;

4 NITROPHENOXYCARBOXYLIC ACID DERIVATIVE; CYTOCHROME P450; CYTOCHROME P450 BM3; CYTOCHROME P450 F87A; UNCLASSIFIED DRUG;

ARTICLE; BINDING KINETICS; COLORIMETRY; ENZYME ASSAY; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GENETIC ENGINEERING; HYDROXYLATION; NONHUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; SPECTROPHOTOMETRY; STRAIN DIFFERENCE;

BACILLUS MEGATERIUM; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STAPHYLOCOCCUS PHAGE 3A;

EID: 0344348884     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4047     Document Type: Article

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