Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes

Drug Metabolism and Disposition

Volumn 32, Issue 7, 2004, Pages 699-706

  Donato, M Teresa ^a   Jiménez, Nuria ^a   Castell, José V ^a   Gómez Lechón, M José ^a  

^a HOSPITAL UNIVERSITARIO LA FE   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

3 [2 (N,N DIETHYL N METHYLAMMONIUM)ETHYL] 7 METHOXY 4 METHYLCOUMARIN; 3 CYANO 7 ETHOXYCOUMARIN; 7 BENZYLOXY 4 TRIFLUOROMETHYLCOUMARIN; 7 ETHOXY 4 TRIFLUOROMETHYLCOUMARIN; 7 METHOXY 4 TRIFLUOROMETHYLCOUMARIN; BENZYL DERIVATIVE; CHLORZOXAZONE; COUMARIN DERIVATIVE; CYTOCHROME P450; CYTOCHROME P450 1A2; CYTOCHROME P450 2A6; CYTOCHROME P450 2B6; CYTOCHROME P450 2C19; CYTOCHROME P450 2C8; CYTOCHROME P450 2C9; CYTOCHROME P450 2D6; CYTOCHROME P450 2E1; CYTOCHROME P450 3A4; DEXTROMETHORPHAN; DIBENZYLFLUORESCEIN; DICLOFENAC; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; MEPHENYTOIN; METHOXSALEN; NITRILE; PACLITAXEL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TESTOSTERONE; UNCLASSIFIED DRUG; UNINDEXED DRUG;

ARTICLE; ASSAY; CELL FRACTIONATION; DRUG METABOLISM; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; FLUOROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LIVER MICROSOME; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING; SENSITIVITY ANALYSIS;

CELLS, CULTURED; CYTOCHROME P-450 ENZYME SYSTEM; FLUORESCENT DYES; FLUOROMETRY; HEPATOCYTES; HUMANS; MICROSOMES, LIVER; TIME FACTORS;

EID: 3042544349     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: 10.1124/dmd.32.7.699     Document Type: Article

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