Development of a fed-batch process for the production of the cytochrome P450 monooxygenase CYP102A1 from Bacillus megaterium in E. coli

Journal of Biotechnology

Volumn 129, Issue 3, 2007, Pages 481-488

  Pflug, Simon ^a   Richter, Sven M ^a   Urlacher, Vlada B ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

CYP102A1; Cytochrome P450 monooxygenase; Fed batch cultivation; Heterologous expression in E. coli

Indexed keywords

BIOREACTORS; BIOSYNTHESIS; ENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; GLYCEROL; SOLUBILITY;

BACILLUS MEGATERIUM; FED BATCH CULTIVATION; RECOMBINANT CYTOCHROME;

BATCH CELL CULTURE;

CARBON; CYTOCHROME P450; CYTOCHROME P450 BM3; GLUCOSE; GLYCEROL; RECOMBINANT ENZYME;

ARTICLE; BACILLUS MEGATERIUM; BIOREACTOR; CULTURE MEDIUM; ENZYME ACTIVITY; ESCHERICHIA COLI; FED BATCH CULTURE; GENE EXPRESSION SYSTEM; HETEROLOGOUS EXPRESSION; NONHUMAN; PARAMETER; PRIORITY JOURNAL; SOLUBILITY;

BACILLUS MEGATERIUM; BACTERIAL PROTEINS; BIOREACTORS; BIOTECHNOLOGY; CELL COUNT; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; MIXED FUNCTION OXYGENASES; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY;

BACILLUS MEGATERIUM;

EID: 34147155935     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.01.013     Document Type: Article

References (24)

1. Boddupalli S.S., Estabrook R.W., and Peterson J.A. Fatty acid monooxygenation by cytochrome P-450 BM-3. J. Biol. Chem. 265 (1990) 4233-4239
2. Carmichael A.B., and Wong L.L. Protein engineering of Bacillus megaterium CYP102-the oxidation of polycyclic aromatic hydrocarbons. Eur. J. Biochem. 268 (2001) 3117-3125
3. Choi J.H., Lee S.J., Lee S.J., and Lee S.Y. Enhanced production of insulin-like growth factor I fusion protein in Escherichia coli by coexpression of the down-regulated genes identified by transcriptome profiling. Appl. Environ. Microb. 69 (2003) 4737-4742
4. Cirino P.C., and Arnold F.H. Regioselectivity and activity of cytochrome P450 BM-3 and mutant F87A in reactions driven by hydrogen peroxide. Adv. Synth. Catal. 344 (2002) 932-937
5. Farinas E.T., Schwaneberg U., Glieder A., and Arnold F.H. Directed evolution of a cytochrome P450 monooxygenase for alkane oxidation. Adv. Synth. Catal. 343 (2001) 601-606
6. Gustafsson M.C.U., Roitel O., Marshall K.R., Noble M.A., Chapman S.K., Pessegueiro A., Fulco A.J., Cheesman M.R., von Wachenfeldt C., and Munro A.W. Expression, purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and CYP102A3: flavocytochrome homologues of P450 BM3 from Bacillus megaterium. Biochemistry 43 (2004) 5474-5487
7. Laemmli U.K. Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature 227 (1970) 680-685
8. Lee S.Y. High cell-density culture of Escherichia coli. Trends Biotechnol. 14 (1996) 98-105
9. Li Q.S., Schwaneberg U., Fischer P., and Schmid R.D. Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst. Chem. Eur. J. 6 (2000) 1481-1486
10. Li Q.S., Ogawa J., Schmid R.D., and Shimizu S. Residue size at position 87 of cytochrome P450 BM-3 determines its stereoselectivity in propylbenzene and 3-chlorostyrene oxidation. FEBS Lett. 508 (2001) 249-252
11. Liu M.Z., Durfee T., Cabrera J.E., Zhao K., Jin D.J., and Blattner F.R. Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli. J. Biol. Chem. 280 (2005) 15921-15927
12. Maurer S.C., Kuhnel K., Kaysser L.A., Eiben S., Schmid R.D., and Urlacher V.B. Catalytic hydroxylation in biphasic systems using CYP102A1 mutants. Adv. Synth. Catal. 347 (2005) 1090-1098
13. Maurer S.C., Schulze H., Schmid R.D., and Urlacher V. Immobilisation of P450 BM-3 and an NADP(+) cofactor recycling system: towards a technical application of heme-containing monooxygenases in fine chemical synthesis. Adv. Synth. Catal. 345 (2003) 802-810
14. Miles J.S., Munro A.W., Rospendowski B.N., Smith W.E., Mcknight J., and Thomson A.J. Domains of the catalytically self-sufficient cytochrome P-450 BM-3-genetic construction, overexpression, purification and spectroscopic characterization. Biochem. J. 288 (1992) 503-509
15. Noble M.A., Miles C.S., Chapman S.K., Lysek D.A., Mackay A.C., Reid G.A., Hanzlik R.P., and Munro A.W. Roles of key active-site residues in flavocytochrome P450 BM3. Biochem. J. 339 (1999) 371-379
16. Omura T., and Sato R. The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J. Biol. Chem. 239 (1964) 2370-2378
17. Ortiz de Montellano P.R. Cytochrome P450: Structure, Mechanism, and Biochemistry (2005), Kluwer Academic/Plenum Publishers, New York
18. Qing G.L., Ma L.C., Khorchid A., Swapna G.V.T., Mal T.K., Takayama M.M., Xia B., Phadtare S., Ke H.P., Acton T., Montelione G.T., Ikura M., and Inouye M. Cold-shock induced high-yield protein production in Escherichia coli. Nat. Biotechnol. 22 (2004) 877-882
19. Schein C.H. A cool way to make proteins. Nat. Biotechnol. 22 (2004) 826-827
20. Schwaneberg U., Schmidt-Dannert C., Schmitt J., and Schmid R.D. A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A. Anal. Biochem. 269 (1999) 359-366
21. Urlacher V.B., Lutz-Wahl S., and Schmid R.D. Microbial P450 enzymes in biotechnology. Appl. Microbiol. Biotechnol. 64 (2004) 317-325
22. Vail R.B., Homann M.J., Hanna I., and Zaks A. Preparative synthesis of drug metabolites using human cytochrome P450s 3A4, 2C9 and 1A2 with NADPH-P450 reductase expressed in Escherichia coli. J. Ind. Microbiol. Biotechnol. 32 (2005) 67-74
23. Wen L.P., and Fulco A.J. Cloning of the gene encoding a catalytically self-sufficient cytochrome P-450 fatty-acid monooxygenase induced by barbiturates in Bacillus megaterium and its functional expression and regulation in heterologous (Escherichia coli) and homologous (Bacillus megaterium) hosts. J. Biol. Chem. 262 (1987) 6676-6682
24. Zwaig N., Kistler W.S., and Lin E.C.C. Glycerol kinase, the pacemaker for the dissimilation of glycerol in Escherichia coli. J. Bacteriol. 102 (1970) 753-759