Fermentative succinate production: An emerging technology to replace the traditional petrochemical processes

BioMed Research International

Volumn 2013, Issue , 2013, Pages

  Cao, Yujin ^a   Zhang, Rubing ^a   Sun, Chao ^a   Cheng, Tao ^a   Liu, Yuhua ^a   Xian, Mo ^a  

^a QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

PETROLEUM; SUCCINIC ACID DERIVATIVE;

BIOMASS; CHEMISTRY; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; REVIEW; SACCHAROMYCES CEREVISIAE;

BIOMASS; PETROLEUM; SACCHAROMYCES CEREVISIAE; SUCCINATES;

EID: 84893719710     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2013/723412     Document Type: Review

References (87)

1. Dougall D. K., Weyrauch K. W., Abilities of organic acids to support growth and anthocyanin accumulation by suspension cultures of wild carrot cells using ammonium as the sole nitrogen source. In (Pubitemid 11180197)
2. Chimirri F., Bosco F., Ceccarelli R., Venturello A., Geobaldo F., Succinic acid and its derivatives: fermentative production using sustainable industrial agro-food by-products and its applications in the food industry. Italian Journal of Food Science 2010 22 2 119 125 2-s2.0-77957906957
3. Sener A., Kadiata M. M., Ladrière L., Malaisse W. J., Synergistic insulinotropic action of succinate, acetate, and glucose esters in islets from normal and diabetic rats. Endocrine 1997 7 2 151 155 2-s2.0-0031407708 (Pubitemid 28092086)
4. Yu C., Cao Y., Zou H., Xian M., Metabolic engineering of Escherichia coli for biotechnological production of high-value organic acids and alcohols. Applied Microbiology and Biotechnology 2011 89 3 573 583 2-s2.0-79251596379 10.1007/s00253-010-2970-z
5. Xu J., Guo B.-H., Poly (butylene succinate) and its copolymers: research, development and industrialization. Biotechnology Journal 2010 5 11 1149 1163 2-s2.0-78349275395 10.1002/biot.201000136
6. Nattrass L., Aylott M., Higson A., NNFCC Renewable Chemicals Factsheet: Succinic Acid 2013 NNFCC
7. MarketsandMarkets, Succinic Acid Market by Applications & Geography-Global Trends & Forecasts (2011-2016) 2012
8. Polly O. L., Production of succinic acid. United States patent 2533620, 1950
9. 2 Catalysis Today 2003 87 1-4 171 177 2-s2.0-0344946477 10.1016/j.cattod.2003.10.010
10. Hu T., Yin H., Zhang R., Wu H., Jiang T., Wada Y., Gas phase hydrogenation of maleic anhydride to γ -butyrolactone by Cu-Zn-Ti catalysts. Catalysis Communications 2007 8 2 193 199 2-s2.0-33845948438 10.1016/j.catcom.2006.06.009 (Pubitemid 46038840)
11. Muzumdar A. V., Sawant S. B., Pangarkar V. G., Reduction of maleic acid to succinic acid on titanium cathode. Organic Process Research and Development 2004 8 4 685 688 2-s2.0-4243175861 10.1021/op0300185 (Pubitemid 39107515)
12. Vitcha J. F., Lester W., Preparation of succinic acid. United States Patent 3341578, 1967
13. Werpy T., Petersen G., Top Value Added Chemicals from Biomass. Volume 1: Results of Screening for Potential Candidates from Sugars and Synthesis Gas 2004 U.S. Department of Energy
14. Song H., Lee S. Y., Production of succinic acid by bacterial fermentation. Enzyme and Microbial Technology 2006 39 3 352 361 2-s2.0-33747280991 10.1016/j.enzmictec.2005.11.043
15. Lin H., Bennett G. N., San K.-Y., Genetic reconstruction of the aerobic central metabolism in Escherichia coli for the absolute aerobic production of succinate. Biotechnology and Bioengineering 2005 89 2 148 156 2-s2.0-12744254193 10.1002/bit.20298
16. Raab A. M., Gebhardt G., Bolotina N., Weuster-Botz D., Lang C., Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid. Metabolic Engineering 2010 12 6 518 525 2-s2.0-78049430020 10.1016/j.ymben.2010.08.005
17. Litsanov B., Kabus A., Brocker M., Bott M., Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum. Microbial Biotechnology 2012 5 1 116 128 2-s2.0-83655197763 10.1111/j.1751-7915. 2011.00310.x
18. Meijer S., Otero J., Olivares R., Andersen M. R., Olsson L., Nielsen J., Overexpression of isocitrate lyase-glyoxylate bypass influence on metabolism in Aspergillus niger. Metabolic Engineering 2009 11 2 107 116 2-s2.0-60849114730 10.1016/j.ymben.2008.12.002
19. Chen K., Jiang M., Wei P., Yao J., Wu H., Succinic acid production from acid hydrolysate of corn fiber by Actinobacillus succinogenes. Applied Biochemistry and Biotechnology 2010 160 2 477 485 2-s2.0-77949297168 10.1007/s12010-008-8367-0
20. Song H., Jang S. H., Park J. M., Lee S. Y., Modeling of batch fermentation kinetics for succinic acid production by Mannheimia succiniciproducens. Biochemical Engineering Journal 2008 40 1 107 115 2-s2.0-42549135463 10.1016/j.bej.2007.11.021
21. Lee P. C., Lee W. G., Lee S. Y., Chang H. N., Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. Biotechnology and Bioengineering 2001 72 1 41 48 (Pubitemid 32085891)
22. Ling E. T. M., Dibble J. T., Houston M. R., Accumulation of 1-trans-2,3-epoxysuccinic acid and succinic acid by Paecilomyces varioti. Applied and Environmental Microbiology 1978 35 6 1213 1215 2-s2.0-0017840767 (Pubitemid 8362611)
23. Bercovitz A., Peleg Y., Battat E., Rokem J. S., Goldberg I., Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains. Applied and Environmental Microbiology 1990 56 6 1594 1597 2-s2.0-0025301129 (Pubitemid 20202117)
24. Gallmetzer M., Meraner J., Burgstaller W., Succinate synthesis and excretion by Penicillium simplicissimum under aerobic and anaerobic conditions. FEMS Microbiology Letters 2002 210 2 221 225 2-s2.0-0037035417 10.1016/S0378-1097(02)00605-5 (Pubitemid 34607189)
25. Coustou V., Besteiro S., Rivière L., Biran M., Biteau N., Franconi J.-M., Boshart M., Baltz T., Bringaud F., A mitochondrial NADH-dependent fumarate reductase involved in the production of succinate excreted by procyclic Trypanosoma brucei. Journal of Biological Chemistry 2005 280 17 16559 16570 2-s2.0-20444485724 10.1074/jbc.M500343200 (Pubitemid 41389111)
26. Yang F., Cao Y., Biosynthesis of phloroglucinol compounds in microorganisms-review. Applied Microbiology and Biotechnology 2012 93 2 487 495 2-s2.0-84856262900 10.1007/s00253-011-3712-6
27. Cao Y., Cao Y., Zhao M. a., Biotechnological production of eicosapentaenoic acid: from a metabolic engineering point of view. Process Biochemistry 2012 47 9 1320 1326
28. Okino S., Noburyu R., Suda M., Jojima T., Inui M., Yukawa H., An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain. Applied Microbiology and Biotechnology 2008 81 3 459 464 2-s2.0-56349093759 10.1007/s00253-008-1668-y
29. Wakai Y., Shimazaki T., Hara S., Formation of succinate during fermentation of sake mash and grape must. Hakkokogaku Kaishi-Journal of the Society of Fermentation Technology 1980 58 5 363 368
30. Sun L., Zhang H., Yuan H., Tu R., Wang Q., Ma Y., A double-enzyme-coupled assay for high-throughput screening of succinic acid-producing strains. Journal of Applied Microbiology 2013 114 6 1696 1701
31. Cheng K. K., Wang G. Y., Zeng J., Zhang J. A., Improved succinate production by metabolic engineering. Biomed Research International 2013 2013 12 538790 10.1155/2013/538790
32. Sánchez A. M., Bennett G. N., San K.-Y., Efficient succinic acid production from glucose through overexpression of pyruvate carboxylase in an Escherichia coli alcohol dehydrogenase and lactate dehydrogenase mutant. Biotechnology Progress 2005 21 2 358 365 2-s2.0-16444375254 10.1021/bp049676e (Pubitemid 40473707)
33. Wu H., Li Z.-M., Zhou L., Ye Q., Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture. Applied and Environmental Microbiology 2007 73 24 7837 7843 2-s2.0-37349117342 10.1128/AEM.01546-07 (Pubitemid 350308799)
34. Chatterjee R., Millard C. S., Champion K., Clark D. P., Donnelly M. I., Mutation of the ptsG gene results in increased production of succinate in fermentation of glucose by Escherichia coli. Applied and Environmental Microbiology 2001 67 1 148 154 2-s2.0-0035158424 10.1128/AEM.67.1.148-154.2001 (Pubitemid 32044412)
35. Lee S. J., Lee D.-Y., Kim T. Y., Kim B. H., Lee J., Lee S. Y., Metabolic engineering of Escherichia coli for enhanced production of succinic acid, based on genome comparison and in silico gene knockout simulation. Applied and Environmental Microbiology 2005 71 12 7880 7887 2-s2.0-29144484729 10.1128/AEM.71.12.7880-7887.2005 (Pubitemid 41805090)
36. Liu Z., Wang X., Qi Q., Hua Q., Quantification and analysis of metabolic characteristics of aerobic succinate-producing Escherichia coli under different aeration conditions. Process Biochemistry 2012 47 11 1532 1538 2-s2.0-84857129909 10.1016/j.procbio.2012.01.022
37. Lin H., Bennett G. N., San K.-Y., Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: a study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile. Metabolic Engineering 2005 7 5-6 337 352 2-s2.0-29144444584 10.1016/j.ymben.2005.06.002 (Pubitemid 41794052)
38. Jantama K., Zhang X., Moore J. C., Shanmugam K. T., Svoronos S. A., Ingram L. O., Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C. Biotechnology and Bioengineering 2008 101 5 881 893 2-s2.0-56449105588 10.1002/bit.22005
39. Millard C. S., Chao Y.-P., Liao J. C., Donnelly M. I., Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli. Applied and Environmental Microbiology 1996 62 5 1808 1810 2-s2.0-0029930612 (Pubitemid 26143465)
40. Zhang X., Jantama K., Moore J. C., Jarboe L. R., Shanmugam K. T., Ingram L. O., Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 2009 106 48 20180 20185 2-s2.0-73949115238 10.1073/pnas.0905396106
41. +-dependent malic enzyme in an Escherichia coli mutant. Applied and Environmental Microbiology 1997 63 7 2695 2701 2-s2.0-0030752053 (Pubitemid 27292862)
42. Liang L.-Y., Liu R.-M., Ma J.-F., Chen K.-Q., Jiang M., Wei P., Increased production of succinic acid in Escherichia coli by overexpression of malate dehydrogenase. Biotechnology Letters 2011 33 12 2439 2444 2-s2.0-81155131090 10.1007/s10529-011-0707-4
43. Hong S. H., Lee S. Y., Enhanced production of succinic acid by metabolically engineered Escherichia coli with amplified activities of malic enzyme and fumarase. Biotechnology and Bioprocess Engineering 2004 9 4 252 255 2-s2.0-28344441063
44. Sánchez A. M., Bennett G. N., San K.-Y., Batch culture characterization and metabolic flux analysis of succinate-producing Escherichia coli strains. Metabolic Engineering 2006 8 3 209 226 2-s2.0-33747369422 10.1016/j.ymben.2005.11.004 (Pubitemid 44382420)
45. + ratio by overexpression of nicotinic acid phosphoribosyltransferase for succinic acid production in Escherichia coli NZN111. Enzyme and Microbial Technology 2012 51 5 286 293
46. Kim P., Laivenieks M., Vieille C., Zeikus J. G., Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli. Applied and Environmental Microbiology 2004 70 2 1238 1241 2-s2.0-2342516028 10.1128/AEM.70.2.1238-1241.2004 (Pubitemid 38568256)
47. Arikawa Y., Kuroyanagi T., Shimosaka M., Muratsubaki H., Enomoto K., Kodaira R., Okazaki M., Effect of gene disruptions of the TCA cycle on production of succinic acid in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering 1999 87 1 28 36 2-s2.0-0032952709 10.1016/S1389-1723(99) 80004-8 (Pubitemid 29162227)
48. Otero J. M., Cimini D., Patil K. R., Poulsen S. G., Olsson L., Nielsen J., Industrial systems biology of Saccharomyces cerevisiae enables novel succinic acid cell factory. PLoS ONE 2013 8 1 e54144
49. Litsanov B., Brocker M., Bott M., Toward homosuccinate fermentation: metabolic engineering of Corynebacterium glutamicum for anaerobic production of succinate from glucose and formate. Applied and Environmental Microbiology 2012 78 9 3325 3337
50. Lee S. J., Song H., Lee S. Y., Genome-based metabolic engineering of Mannheimia succiniproducens for succinic acid production. Applied and Environmental Microbiology 2006 72 3 1939 1948 2-s2.0-33645029734 10.1128/AEM.72.3.1939-1948.2006
51. Cao Y., Cao Y., Lin X., Metabolically engineered Escherichia coli for biotechnological production of four-carbon 1,4-dicarboxylic acids. Journal of Industrial Microbiology and Biotechnology 2011 38 6 649 656 2-s2.0-80051664276 10.1007/s10295-010-0913-4
52. Zhu J., Shimizu K., The effect of pfl gene knockout on the metabolism for optically pure D-lactate production by Escherichia coli. Applied Microbiology and Biotechnology 2004 64 3 367 375 2-s2.0-2442545374 10.1007/s00253-003-1499-9 (Pubitemid 38626461)
53. Vemuri G. N., Eiteman M. A., Altman E., Succinate production in dual-phase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions. Journal of Industrial Microbiology and Biotechnology 2002 28 6 325 332 2-s2.0-0036309458 (Pubitemid 34759913)
54. Besteiro S., Biran M., Biteau N., Coustou V., Baltz T., Canioni P., Bringaud F., Succinate secreted by Trypanosoma brucei is produced by a novel and unique glycosomal enzyme, NADH-dependent fumarate reductase. Journal of Biological Chemistry 2002 277 41 38001 38012 2-s2.0-0037064007 10.1074/jbc.M201759200 (Pubitemid 35154651)
55. Lin H., Bennett G. N., San K.-Y., Effect of carbon sources differing in oxidation state and transport route on succinate production in metabolically engineered Escherichia coli. Journal of Industrial Microbiology and Biotechnology 2005 32 3 87 93 2-s2.0-17744383410 10.1007/s10295-005-0206-5 (Pubitemid 40575675)
56. Hong S. H., Lee S. Y., Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli. Applied Microbiology and Biotechnology 2002 58 3 286 290 2-s2.0-0036186330 10.1007/s00253-001-0899-y (Pubitemid 34185570)
57. Chen X., Jiang S., Zheng Z., Pan L., Luo S., Effects of culture redox potential on succinic acid production by Corynebacterium crenatum under anaerobic conditions. Process Biochemistry 2012 47 8 1250 1255
58. Laivenieks M., Vieille C., Zeikus J. G., Cloning, sequencing, and overexpression of the Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase (pckA) gene. Applied and Environmental Microbiology 1997 63 6 2273 2280 2-s2.0-0030958370 (Pubitemid 27232925)
59. Jeon J. M., Thangamani R., Song E., Lee H. W., Lee H. W., Yang Y. H., Media optimization of Corynebacterium glutamicum for succinate production under oxygen-deprived condition. Journal of Microbiology and Biotechnology 2013 23 2 211 217
60. 2 fixation for succinic acid production using Actinobacillus succinogenes. Journal of Industrial Microbiology and Biotechnology 2011 38 9 1605 1612 2-s2.0-80052476331 10.1007/s10295-011-0952-5
61. Lu S., Eiteman M. A., Altman E., PH and base counterion affect succinate production in dual-phase Escherichia coli fermentations. Journal of Industrial Microbiology and Biotechnology 2009 36 8 1101 1109 2-s2.0-68349131757 10.1007/s10295-009-0594-z
62. Zhu J., Thakker C., San K.-Y., Bennett G., Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain. Applied Microbiology and Biotechnology 2011 92 3 499 508 2-s2.0-82355173514 10.1007/s00253-011-3314-3
63. Lin H., Bennett G. N., San K.-Y., Fed-batch culture of a metabolically engineered Escherichia coli strain designed for high-level succinate production and yield under aerobic conditions. Biotechnology and Bioengineering 2005 90 6 775 779 2-s2.0-19744367895 10.1002/bit.20458 (Pubitemid 40744245)
64. Vemuri G. N., Eiteman M. A., Altman E., Effects of growth mode and pyruvate carboxylase on succinic acid production by metabolically engineered strains of Escherichia coli. Applied and Environmental Microbiology 2002 68 4 1715 1727 2-s2.0-0036210809 10.1128/AEM.68.4.1715-1727.2002 (Pubitemid 34284775)
65. Kim D. Y., Yim S. C., Lee P. C., Lee W. G., Lee S. Y., Chang H. N., Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens MBEL55E. Enzyme and Microbial Technology 2004 35 6-7 648 653 2-s2.0-7544233865 10.1016/j.enzmictec.2004.08.018 (Pubitemid 39452727)
66. Lee P. C., Lee S. Y., Chang H. N., Kinetic study on succinic acid and acetic acid formation during continuous cultures of Anaerobiospirillum succiniciproducens grown on glycerol. Bioprocess and Biosystems Engineering 2010 33 4 465 471 2-s2.0-77953610257 10.1007/s00449-009-0355-4
67. van Heerden C. D., Nicol W., Continuous succinic acid fermentation by Actinobacillus succinogenes. Biochemical Engineering Journal 2013 73 5 11
68. de Barros M., Freitas S., Padilha G., Alegre R., Biotechnological production of succinic acid by Actinobacillus succinogenes using different substrate. Chemical Engineering Transactions 2013 32 985 990
69. Zhang X., Shanmugam K. T., Ingram L. O., Fermentation of glycerol to succinate by metabolically engineered strains of Escherichia coli. Applied and Environmental Microbiology 2010 76 8 2397 2401 2-s2.0-77950580812 10.1128/AEM.02902-09
70. Liu R., Liang L., Chen K., Ma J., Jiang M., Wei P., Ouyang P., Fermentation of xylose to succinate by enhancement of ATP supply in metabolically engineered Escherichia coli. Applied Microbiology and Biotechnology 2012 94 4 959 968 2-s2.0-84856271430 10.1007/s00253-012-3896-4
71. Li X., Zheng Z., Wei Z., Jiang S., Pan L., Weng S., Screening, breeding and metabolic modulating of a strain producing succinic acid with corn straw hydrolyte. World Journal of Microbiology and Biotechnology 2009 25 4 667 677 2-s2.0-61849130651 10.1007/s11274-008-9936-7
72. Li Q., Siles J. A., Thompson I. P., Succinic acid production from orange peel and wheat straw by batch fermentations of Fibrobacter succinogenes S85. Applied Microbiology and Biotechnology 2010 88 3 671 678 2-s2.0-78149359673 10.1007/s00253-010-2726-9
73. Zheng P., Fang L., Xu Y., Dong J.-J., Ni Y., Sun Z.-H., Succinic acid production from corn stover by simultaneous saccharification and fermentation using Actinobacillus succinogenes. Bioresource Technology 2010 101 20 7889 7894 2-s2.0-77954311736 10.1016/j.biortech.2010.05.016
74. Borges E. R., Pereira N. Jr., Succinic acid production from sugarcane bagasse hemicellulose hydrolysate by Actinobacillus succinogenes. Journal of Industrial Microbiology and Biotechnology 2011 38 8 1001 1011 2-s2.0-80051691113 10.1007/s10295-010-0874-7
75. Li Q., He Y.-C., Xian M., Jun G., Xu X., Yang J.-M., Li L.-Z., Improving enzymatic hydrolysis of wheat straw using ionic liquid 1-ethyl-3-methyl imidazolium diethyl phosphate pretreatment. Bioresource Technology 2009 100 14 3570 3575 2-s2.0-64849103083 10.1016/j.biortech.2009.02.040
76. Cheng K. K., Zhao X. B., Zeng J., Downstream processing of biotechnological produced succinic acid. Applied Microbiology and Biotechnology 2012 95 4 841 850
77. McKinlay J. B., Vieille C., Zeikus J. G., Prospects for a bio-based succinate industry. Applied Microbiology and Biotechnology 2007 76 4 727 740 2-s2.0-34548317397 10.1007/s00253-007-1057-y (Pubitemid 47340937)
78. Li Q., Wang D., Wu Y., Li W., Zhang Y., Xing J., Su Z., One step recovery of succinic acid from fermentation broths by crystallization. Separation and Purification Technology 2010 72 3 294 300 2-s2.0-77950942199 10.1016/j.seppur.2010.02.021
79. Huh Y. S., Jun Y.-S., Hong Y. K., Song H., Lee S. Y., Hong W. H., Effective purification of succinic acid from fermentation broth produced by Mannheimia succiniciproducens. Process Biochemistry 2006 41 6 1461 1465 2-s2.0-33646550894 10.1016/j.procbio.2006.01.020
80. Glassner D. A., Elankovan P., Beacom D. R., Berglund K. A., Purification process for succinic acid produced by fermentation. Applied Biochemistry and Biotechnology 1995 51-52 1 73 82 2-s2.0-0002067197 10.1007/BF02933412
81. Nam H.-G., Park K.-M., Lim S. S., Mun S., Adsorption equilibria of succinic acid and lactic acid on amberchrom CG300C resin. Journal of Chemical and Engineering Data 2011 56 3 464 471 2-s2.0-79952652293 10.1021/je1008729
82. Li Q., Xing J., Li W., Liu Q., Su Z., Separation of succinic acid from fermentation broth using weak alkaline anion exchange adsorbents. Industrial and Engineering Chemistry Research 2009 48 7 3595 3599 2-s2.0-65249146639 10.1021/ie801304k
83. 2 for reversible pH shifting, and the removal of succinic acid in a polymer-based two-phase partitioning bioreactor. Journal of Chemical Technology and Biotechnology 2012 87 1 42 50 2-s2.0-83555179062 10.1002/jctb.2763
84. Fruchey O. S., Keen B. T., Albin B. A., Clinton N. A., Dunuwila D., Dombek B. D., Processes for producing succinic acid from fermentation broths containing diammonium succinate. United States patent 20110297527, 2011
85. Cheng K.-K., Zhao X.-B., Zeng J., Zhang J.-A., Biotechnological production of succinic acid: current state and perspectives. Biofuels, Bioproducts and Biorefining 2012 6 3 302 318 2-s2.0-84857499372 10.1002/bbb.1327
86. Beauprez J. J., De Mey M., Soetaert W. K., Microbial succinic acid production: natural versus metabolic engineered producers. Process Biochemistry 2010 45 7 1103 1114 2-s2.0-77954834802 10.1016/j.procbio.2010.03.035
87. Jarboe L. R., Zhang X. L., Wang X., Moore J. C., Shanmugam K. T., Ingram L. O., Metabolic engineering for production of biorenewable fuels and chemicals: contributions of synthetic biology. Journal of Biomedicine and Biotechnology 2010 2010 18 761042 10.1155/2010/761042