A double-enzyme-coupled assay for high-throughput screening of succinic acid-producing strains

Journal of Applied Microbiology

Volumn 114, Issue 6, 2013, Pages 1696-1701

  Sun, L ^a,b   Zhang, H ^a   Yuan, H ^a   Tu, R ^a   Wang, Q ^a   Ma, Y ^a  

^a Tianjin Airport Economic Area   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Double enzyme coupled assay; High throughput screening; Metabolites; Microplate assay; Succinic acid

Indexed keywords

BACTERIA; ENZYMES; FERMENTATION; THROUGHPUT;

ACID PRODUCING; ACID PRODUCING BACTERIUM; DETECTION ASSAY; DOUBLE-ENZYME-COUPLED ASSAY; FERMENTATION TIME; HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT ASSAYS; MICROPLATE ASSAY; MICROPLATES; SUCCINIC ACIDS;

METABOLITES;

ACETIC ACID; ADIPIC ACID; FORMIC ACID; FUMARATE REDUCTASE; FUMARIC ACID; GLUTARIC ACID; MALONIC ACID; PEROXIDASE; SUCCINIC ACID;

BIOASSAY; CARBOXYLIC ACID; ENZYME ACTIVITY; FERMENTATION; GENETIC ANALYSIS; METABOLITE; MICROBIAL COMMUNITY;

ARTICLE; BACTERIAL STRAIN; CLONING; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME SPECIFICITY; ESCHERICHIA COLI; FERMENTATION; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; NONHUMAN;

EID: 84878124646     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12175     Document Type: Article

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