-
1
-
-
1942503297
-
Controlling the enantioselectivity of enzymes by directed evolution: Practical and theoretical ramifications
-
Reetz, M. T. (2004) Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications, Proc. Natl. Acad. Sci. U.S.A. 101, 5716-5722.
-
(2004)
Proc. Natl. Acad. Sci. U.S.A.
, vol.101
, pp. 5716-5722
-
-
Reetz, M.T.1
-
2
-
-
0038625074
-
Mimicking natural evolution in vitro: An N-acetylneuraminate lyase mutant with an increased dihydrodipicolinate synthase activity
-
Joerger, A. C., Mayer, S., and Fersht, A. R. (2003) Mimicking natural evolution in vitro: An N-acetylneuraminate lyase mutant with an increased dihydrodipicolinate synthase activity, Proc. Natl. Acad. Sci. U.S.A. 100, 5694-5699.
-
(2003)
Proc. Natl. Acad. Sci. U.S.A.
, vol.100
, pp. 5694-5699
-
-
Joerger, A.C.1
Mayer, S.2
Fersht, A.R.3
-
3
-
-
0041429626
-
Conversion of a PLP-dependent racemase into an aldolase by a single active site mutation
-
Seebeck, F. P., and Hilvert, D. (2003) Conversion of a PLP-dependent racemase into an aldolase by a single active site mutation, J. Am. Chem. Soc. 125, 10158-10159.
-
(2003)
J. Am. Chem. Soc.
, vol.125
, pp. 10158-10159
-
-
Seebeck, F.P.1
Hilvert, D.2
-
4
-
-
3042655537
-
Computational design of a biologically active enzyme
-
Dwyer, M. A., Looger, L. L., and Hellinga, H. W. (2004) Computational design of a biologically active enzyme, Science 304, 196-197.
-
(2004)
Science
, vol.304
, pp. 196-197
-
-
Dwyer, M.A.1
Looger, L.L.2
Hellinga, H.W.3
-
5
-
-
4544371858
-
Catalytic antibodies: Hapten design strategies and screening methods
-
Xu, Y., Yamamoto, N., and Janda, K. (2004) Catalytic antibodies: hapten design strategies and screening methods, Bioorg. Med. Chem. 12, 5247-5268.
-
(2004)
Bioorg. Med. Chem.
, vol.12
, pp. 5247-5268
-
-
Xu, Y.1
Yamamoto, N.2
Janda, K.3
-
6
-
-
0036882403
-
Catalytic antibodies as designer proteases and esterases
-
Tanaka F. (2002) Catalytic antibodies as designer proteases and esterases, Chem. Rev. 102, 4885-4906.
-
(2002)
Chem. Rev.
, vol.102
, pp. 4885-4906
-
-
Tanaka, F.1
-
7
-
-
0036837384
-
Reactive immunization: A unique approach to catalytic antibodies
-
Tanaka, F., and Barbas, C. F., III (2002) Reactive immunization: a unique approach to catalytic antibodies, J. Immunol. Methods 269, 67-79.
-
(2002)
J. Immunol. Methods
, vol.269
, pp. 67-79
-
-
Tanaka, F.1
Barbas III, C.F.2
-
8
-
-
0027453389
-
Synthesis, structure and activity of artificial, rationally designed catalytic polypeptides
-
Johnsson, K., Allemann, R. K., Widmer, H., and Benner, S. A. (1993) Synthesis, structure and activity of artificial, rationally designed catalytic polypeptides, Nature 365, 530-532.
-
(1993)
Nature
, vol.365
, pp. 530-532
-
-
Johnsson, K.1
Allemann, R.K.2
Widmer, H.3
Benner, S.A.4
-
9
-
-
0030047220
-
Functionalized protein-like structures from conformationlly defined synthetic combinatorial libraries
-
Perez-Paya, E., Houghten, R. A., and Blondell, S. E. (1996) Functionalized protein-like structures from conformationlly defined synthetic combinatorial libraries, J. Biol Chem. 277, 4120-4126.
-
(1996)
J. Biol Chem.
, vol.277
, pp. 4120-4126
-
-
Perez-Paya, E.1
Houghten, R.A.2
Blondell, S.E.3
-
10
-
-
0002689549
-
A designed folded polypeptide model system that catalyzes the decarboxylation of oxaloacetate
-
Allert, M., Kjellstrand, M., Broo, K., Nilsson, A., and Baltzer, L. (1998) A designed folded polypeptide model system that catalyzes the decarboxylation of oxaloacetate, J. Chem. Soc., Perkin Trans. 2, 2271-2274.
-
(1998)
J. Chem. Soc., Perkin Trans.
, vol.2
, pp. 2271-2274
-
-
Allert, M.1
Kjellstrand, M.2
Broo, K.3
Nilsson, A.4
Baltzer, L.5
-
11
-
-
0037013595
-
Setting the stage for new catalytic functions in designed proteins - Exploring the imine pathway in the efficient decarboxylation of oxaloacetate by an Arg ± Lys site in a four-helix bundle protein scaffold
-
Allert, M., and Baltzer, L. (2002) Setting the stage for new catalytic functions in designed proteins - Exploring the imine pathway in the efficient decarboxylation of oxaloacetate by an Arg ± Lys site in a four-helix bundle protein scaffold, Chem. Eur. J. 8, 2549-2560.
-
(2002)
Chem. Eur. J.
, vol.8
, pp. 2549-2560
-
-
Allert, M.1
Baltzer, L.2
-
12
-
-
4544351563
-
A stable miniature protein with oxaloacetate decarboxylase activity
-
Weston, C. J., Cureton, C. H., Calvert, M. J., Smart, O. S., and Allemann, R. K. (2004) A stable miniature protein with oxaloacetate decarboxylase activity, ChemBioChem 5, 1075-1080.
-
(2004)
ChemBioChem
, vol.5
, pp. 1075-1080
-
-
Weston, C.J.1
Cureton, C.H.2
Calvert, M.J.3
Smart, O.S.4
Allemann, R.K.5
-
13
-
-
0031587461
-
Catalysis of hydrolysis and transesterification reactions of p-nitrophenyl esters by a designed helix-loop-helix dimer
-
Broo, K., Brive, L., Ahlberg, P., and Baltzer, L. (1997) Catalysis of hydrolysis and transesterification reactions of p-nitrophenyl esters by a designed helix-loop-helix dimer, J. Am. Chem. Soc. 119, 11362-11372.
-
(1997)
J. Am. Chem. Soc.
, vol.119
, pp. 11362-11372
-
-
Broo, K.1
Brive, L.2
Ahlberg, P.3
Baltzer, L.4
-
14
-
-
0034674345
-
a of reactive esters sets the stage for cooperativity in nucleophilic and general-acid catalysis
-
a of reactive esters sets the stage for cooperativity in nucleophilic and general-acid catalysis, Chem. Eur. J. 6, 2214-2220.
-
(2000)
Chem. Eur. J.
, vol.6
, pp. 2214-2220
-
-
Nilsson, J.1
Baltzer, L.2
-
15
-
-
3042546385
-
Selective catalysis with peptide dendrimers
-
Douat-Casassus, C., Darbre, T., and Reymond, J.-L. (2004) Selective catalysis with peptide dendrimers, J. Am. Chem. Soc. 126, 7817-7816.
-
(2004)
J. Am. Chem. Soc.
, vol.126
, pp. 7817-17816
-
-
Douat-Casassus, C.1
Darbre, T.2
Reymond, J.-L.3
-
16
-
-
0842270116
-
Enzyme-like proteins from an unselected library of designed amino acid sequences
-
Wei, Y., and Hecht, M. H. (2004) Enzyme-like proteins from an unselected library of designed amino acid sequences, Protein Eng. Des. Sel. 17, 67-75.
-
(2004)
Protein Eng. Des. Sel.
, vol.17
, pp. 67-75
-
-
Wei, Y.1
Hecht, M.H.2
-
17
-
-
0344443346
-
Molecular recognition of protein surfaces: High affinity ligands for the CBP KIX domain
-
Rutledge, S. E., Volkman, H. M., and Schepartz, A. (2003) Molecular recognition of protein surfaces: high affinity ligands for the CBP KIX domain, J. Am. Chem. Soc. 125, 14336-14336.
-
(2003)
J. Am. Chem. Soc.
, vol.125
, pp. 14336-14336
-
-
Rutledge, S.E.1
Volkman, H.M.2
Schepartz, A.3
-
18
-
-
0017388568
-
Determination of dissociation and Michaelis constants at near-equal enzyme-substrate concentrations
-
Smith, G. D., Eisenthal, R., and Harrison, R. (1977) Determination of dissociation and Michaelis constants at near-equal enzyme-substrate concentrations, Anal. Biochem. 79, 643-647.
-
(1977)
Anal. Biochem.
, vol.79
, pp. 643-647
-
-
Smith, G.D.1
Eisenthal, R.2
Harrison, R.3
-
19
-
-
0035850820
-
Observation of covalent intermediates in an enzyme mechanism at atomic resolution
-
Heine, A., DeSantis, G., Luz, J. G., Mitchell, M., Wong, C. H., and Wilson, I. A. (2001) Observation of covalent intermediates in an enzyme mechanism at atomic resolution, Science 294, 369-374.
-
(2001)
Science
, vol.294
, pp. 369-374
-
-
Heine, A.1
DeSantis, G.2
Luz, J.G.3
Mitchell, M.4
Wong, C.H.5
Wilson, I.A.6
-
20
-
-
0035957423
-
Covalent intermediate trapped in 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase structure at 1.95-Å resolution
-
Allard, J., Grochulski, P., and Sygusch, J. (2001) Covalent intermediate trapped in 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase structure at 1.95-Å resolution, Proc. Natl. Acad. Sci. U.S.A. 98, 3679-3684.
-
(2001)
Proc. Natl. Acad. Sci. U.S.A.
, vol.98
, pp. 3679-3684
-
-
Allard, J.1
Grochulski, P.2
Sygusch, J.3
-
21
-
-
0035923437
-
Snapshots of catalysis: The structure of fructose-1,6-(bis)phosphate aldolase covalently bound to the substrate dihydroxyacetone phosphate
-
Choi, K. H., Shi, J., Hopkins, C. E., Tolan, D. R., and Allen, K. N. (2001) Snapshots of catalysis: the structure of fructose-1,6-(bis)phosphate aldolase covalently bound to the substrate dihydroxyacetone phosphate, Biochemistry 40, 13868-13875.
-
(2001)
Biochemistry
, vol.40
, pp. 13868-13875
-
-
Choi, K.H.1
Shi, J.2
Hopkins, C.E.3
Tolan, D.R.4
Allen, K.N.5
-
22
-
-
0027285118
-
A data-based reaction mechanism for type I fructose bisphosphate aldolase
-
Littlechild, J. A., and Watson, H. C. (1993) A data-based reaction mechanism for type I fructose bisphosphate aldolase, Trends Biochem. Sci. 18, 36-39.
-
(1993)
Trends Biochem. Sci.
, vol.18
, pp. 36-39
-
-
Littlechild, J.A.1
Watson, H.C.2
-
23
-
-
0034679007
-
Reconstructing aldolase antibodies to alter their substrate specificity and turnover
-
Tanaka, F., Lerner, R. A., and Barbas, C. F., III (2000) Reconstructing aldolase antibodies to alter their substrate specificity and turnover, J. Am. Chem. Soc. 122, 4835-4836.
-
(2000)
J. Am. Chem. Soc.
, vol.122
, pp. 4835-4836
-
-
Tanaka, F.1
Lerner, R.A.2
Barbas III, C.F.3
-
24
-
-
0345862284
-
Evolution of aldolase antibodies in vitro: Correlation of catalytic activity and reaction-based selection
-
Tanaka, F., Fuller, R., Shim, H., Lerner, R. A., and Barbas, C. F., III (2004) Evolution of aldolase antibodies in vitro: correlation of catalytic activity and reaction-based selection, J. Mol. Biol. 335, 1007-1018.
-
(2004)
J. Mol. Biol.
, vol.335
, pp. 1007-1018
-
-
Tanaka, F.1
Fuller, R.2
Shim, H.3
Lerner, R.A.4
Barbas III, C.F.5
-
25
-
-
0016765041
-
Modification of arginine and lysine in proteins
-
Gilbert, H. F., III, and O'Leary, M. H. (1975) Modification of arginine and lysine in proteins, Biochemistry 14, 5194-5199.
-
(1975)
Biochemistry
, vol.14
, pp. 5194-5199
-
-
Gilbert III, H.F.1
O'Leary, M.H.2
-
26
-
-
0018783090
-
Modification of lysyl residues of dihydrofolate reductase with 2,4-pentanedione
-
Otwell, H. B., Cipollo, K. L., and Dunlap, R. B. (1979) Modification of lysyl residues of dihydrofolate reductase with 2,4-pentanedione, Biochim. Biophys. Acta 568, 297-306.
-
(1979)
Biochim. Biophys. Acta
, vol.568
, pp. 297-306
-
-
Otwell, H.B.1
Cipollo, K.L.2
Dunlap, R.B.3
-
27
-
-
0029590066
-
Efficient aldolase catalytic antibodies that use the enamine mechanism of natural enzymes
-
Wagner, J., Lerner, R. A., and Barbas, C. F., III (1995) Efficient aldolase catalytic antibodies that use the enamine mechanism of natural enzymes. Science 270, 1797-1800.
-
(1995)
Science
, vol.270
, pp. 1797-1800
-
-
Wagner, J.1
Lerner, R.A.2
Barbas III, C.F.3
-
28
-
-
0035926124
-
Phage display selection of peptides possessing aldolase activity
-
Tanaka, F., and Barbas, C. F., III (2001) Phage display selection of peptides possessing aldolase activity, Chem. Commun., 769-770.
-
(2001)
Chem. Commun.
, pp. 769-770
-
-
Tanaka, F.1
Barbas III, C.F.2
-
29
-
-
0022977611
-
Amino acid sequence of the pyruvate and the glyoxylate active-site lysine peptide of Escherichia coli 2-keto-4-hydroxyglutarate aldolase
-
Vlahos, C, J., and Dekker, E. E. (1986) Amino acid sequence of the pyruvate and the glyoxylate active-site lysine peptide of Escherichia coli 2-keto-4-hydroxyglutarate aldolase, J. Biol. Chem. 261, 11049-11055.
-
(1986)
J. Biol. Chem.
, vol.261
, pp. 11049-11055
-
-
Vlahos, C.J.1
Dekker, E.E.2
-
30
-
-
0029959359
-
Copying nature's mechanism for the decarboxylation of β-keto acid into catalytic antibodies by reactive immunization
-
Bjornestedt, R., Zhong, G., Lerner, R. A., and Barbas, C. F., III (1996) Copying nature's mechanism for the decarboxylation of β-keto acid into catalytic antibodies by reactive immunization, J. Am. Chem. Soc. 118, 11720-11724.
-
(1996)
J. Am. Chem. Soc.
, vol.118
, pp. 11720-11724
-
-
Bjornestedt, R.1
Zhong, G.2
Lerner, R.A.3
Barbas III, C.F.4
-
31
-
-
0003400039
-
-
Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
-
Barbas, C. F., III, Burton, D. R., Scott, J. K., and Silverman, G. J., Eds. (2001) Phage Display: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
-
(2001)
Phage Display: A Laboratory Manual
-
-
Barbas III, C.F.1
Burton, D.R.2
Scott, J.K.3
Silverman, G.J.4
-
32
-
-
0003998388
-
-
CRC Press, New York
-
Lide, D. R., Ed. (2002) CRC Handbook of Chemistry and Physics, 83rd ed., p 7-1, CRC Press, New York.
-
(2002)
CRC Handbook of Chemistry and Physics, 83rd Ed.
, pp. 7-11
-
-
Lide, D.R.1
-
33
-
-
0033533131
-
Thiazolium-dependent catalytic antibodies produced using a covalent modification strategy
-
Tanaka, F., Lerner, R. A., and Barbas, C. F., III (1999) Thiazolium-dependent catalytic antibodies produced using a covalent modification strategy, Chem. Commun., 1383-1384.
-
(1999)
Chem. Commun.
, pp. 1383-1384
-
-
Tanaka, F.1
Lerner, R.A.2
Barbas III, C.F.3
-
34
-
-
6344260061
-
The origin of enantioselectivity in aldolase antibodies: Crystal structure, site-directed mutagenesis, and computational analysis
-
Zhu, X., Tanaka, F., Hu, Y., Heine, A., Fuller, R., Zhong, G., Olson, A. J., Lerner, R. A., Barbas, C. F., III, and Wilson, I. A. (2004) The origin of enantioselectivity in aldolase antibodies: crystal structure, site-directed mutagenesis, and computational analysis, J. Mol. Biol. 343, 1269-1280.
-
(2004)
J. Mol. Biol.
, vol.343
, pp. 1269-1280
-
-
Zhu, X.1
Tanaka, F.2
Hu, Y.3
Heine, A.4
Fuller, R.5
Zhong, G.6
Olson, A.J.7
Lerner, R.A.8
Barbas III, C.F.9
Wilson, I.A.10
-
35
-
-
0038375490
-
Fluorescent detection of carbon-carbon bond formation
-
Tanaka, F., Thayumanavan, R., and Barbas, C. F., III (2003) Fluorescent detection of carbon-carbon bond formation, J. Am. Chem. Soc. 125, 8523-8528.
-
(2003)
J. Am. Chem. Soc.
, vol.125
, pp. 8523-8528
-
-
Tanaka, F.1
Thayumanavan, R.2
Barbas III, C.F.3
-
36
-
-
0345735491
-
Rapid analysis of solvent effects on enamine formation by fluorescence: How might enzymes facilitate enamine chemistry with primary amines?
-
Tanaka, F., Thayumanavan, R., Mase, N., and Barbas, C. F., III (2004) Rapid analysis of solvent effects on enamine formation by fluorescence: how might enzymes facilitate enamine chemistry with primary amines?, Tetrahedron Lett. 45, 325-328.
-
(2004)
Tetrahedron Lett.
, vol.45
, pp. 325-328
-
-
Tanaka, F.1
Thayumanavan, R.2
Mase, N.3
Barbas III, C.F.4
-
37
-
-
0034175175
-
Covalent control of polypeptide folding. Induction of helix-loop-helix motifs by bridging
-
Korsgren, P., Ahlberg, P., and Baltzer, L. (2000) Covalent control of polypeptide folding. Induction of helix-loop-helix motifs by bridging, J. Chem. Soc., Perkin Trans. 2, 643-647.
-
(2000)
J. Chem. Soc., Perkin Trans.
, vol.2
, pp. 643-647
-
-
Korsgren, P.1
Ahlberg, P.2
Baltzer, L.3
-
38
-
-
0031457319
-
Immune versus natural selection: Antibody aldolases with enzymic rates but broader scope
-
Barbas, C. F., III, Heine, A., Zhong, G., Hoffmann, T., Gramatikova, S., Bjornestsdt, R., List, B., Anderson, J., Stura, E. A., Wilson, I. A., and Lerner, R. A. (1997) Immune versus natural selection: Antibody aldolases with enzymic rates but broader scope, Science 278, 2085.
-
(1997)
Science
, vol.278
, pp. 2085
-
-
Barbas III, C.F.1
Heine, A.2
Zhong, G.3
Hoffmann, T.4
Gramatikova, S.5
Bjornestsdt, R.6
List, B.7
Anderson, J.8
Stura, E.A.9
Wilson, I.A.10
Lerner, R.A.11
-
39
-
-
0028876808
-
Coincidences, decarboxylation, and electrostatic effects
-
Westheimer, F. H. (1995) Coincidences, decarboxylation, and electrostatic effects, Tetrahedron 51, 3.
-
(1995)
Tetrahedron
, vol.51
, pp. 3
-
-
Westheimer, F.H.1
-
40
-
-
0037051643
-
A modular assembly strategy for improving the substrate specificity of small catalytic peptides
-
Tanaka, F., and Barbas, C. F., III (2002) A modular assembly strategy for improving the substrate specificity of small catalytic peptides, J. Am. Chem. Soc. 124, 3510-3511.
-
(2002)
J. Am. Chem. Soc.
, vol.124
, pp. 3510-3511
-
-
Tanaka, F.1
Barbas III, C.F.2
-
41
-
-
0032403218
-
Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores
-
Rozinov, M. N., and Nolan, G. P. (1998) Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores, Chem. Biol. 5, 713-728.
-
(1998)
Chem. Biol.
, vol.5
, pp. 713-728
-
-
Rozinov, M.N.1
Nolan, G.P.2
-
42
-
-
0028918401
-
A proficient enzyme
-
Radzicka, A., and Wolfenden, R. (1995) A proficient enzyme, Science 267, 90-93.
-
(1995)
Science
, vol.267
, pp. 90-93
-
-
Radzicka, A.1
Wolfenden, R.2
-
43
-
-
0027190754
-
Evolution of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network
-
Andrate, N., Chacon, P., Merelo, J. J., and Moran, F. (1993) Evolution of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network, Protein Eng. 6, 383-390.
-
(1993)
Protein Eng.
, vol.6
, pp. 383-390
-
-
Andrate, N.1
Chacon, P.2
Merelo, J.J.3
Moran, F.4
-
44
-
-
0038023153
-
Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library
-
Cesaro-Tadic, S., Lagos, D., Honegger, A., Rickard, J. H., Partridge, L. J., Blackburn, G. M., and Pluckthun, A. (2003) Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library, Nat. Biotechnol. 21, 679-685.
-
(2003)
Nat. Biotechnol.
, vol.21
, pp. 679-685
-
-
Cesaro-Tadic, S.1
Lagos, D.2
Honegger, A.3
Rickard, J.H.4
Partridge, L.J.5
Blackburn, G.M.6
Pluckthun, A.7
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