Reconstructing aldolase antibodies to alter their substrate specificity and turnover

Journal of the American Chemical Society

Volumn 122, Issue 19, 2000, Pages 4835-4836

  Tanaka, Fujie ^a   Lerner, Richard A ^a   Barbas III, Carlos F ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC ANTIBODY; DIKETONE; FRUCTOSE BISPHOSPHATE ALDOLASE; IMMUNOGLOBULIN HEAVY CHAIN; LYSINE;

AMINO ACID SEQUENCE; ANTIBODY STRUCTURE; ARTICLE; CATALYSIS; CHEMICAL REACTION KINETICS; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME METABOLISM; ENZYME SPECIFICITY; GENE LIBRARY; IMMUNOGLOBULIN VARIABLE REGION;

EID: 0034679007     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0005441     Document Type: Article

References (38)

1. (a) Branden, C.; Tooze, J. Introduction to Protein Structure; Garland Publishing: New York, 1991; p 236.
2. (b) Russell, R. B. J. Mol. Biol. 1998, 279, 1211.
3. (c) Rao, J. K.; Erickson, J. W.; Wlodawer, A. Biochemistry 1991, 30, 4663.
4. (a) Angeles, T. S.; Smith, R. G.; Darsley, M. J.; Sugasawara, R.; Sanchez, R. I.; Kenten, J.; Schultz, P. G.; Martin, M. T. Biochemistry 1993, 32, 12128.
5. (b) Miyashita, H.; Hara, T.; Tanimura, R.; Tanaka, F.; Kikuchi, M.; Fujii, I. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 6045.
6. (c) Fujii, I.; Tanaka, F.; Miyashita, H.; Tanimura, R.; Kinoshita, K. J. Am. Chem. Soc. 1995, 117, 6199.
7. (d) MacBeath, G.; Hilvert, D. Chem. Biol. 1996, 3, 433.
8. (e) Buchbinder, J. L.; Stephenson, R. C.; Scanlan, T. S.; Fletterick, R. J. J. Mol. Biol. 1998, 282, 1033.
9. (f) Charbonnier J.-B.; Golinelli-Pimpaneau, B.; Gigant, B.; Tawfik, D. S.; Chap, R.; Scindler, D. G.; Kim, S.-H.; Green, B. S.; Eshhar, Z.; Knossow, M. Science 1997, 275, 1140.
10. Kaartinen, M.; Solin, M.-L.; Mäkelä, O. Eur. J. Immunol. 1991, 21, 2863.
11. (a) Baca, M.; Scanlan, T. S.; Stephenson, R. C.; Wells, J. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 10063.
12. (b) Fujii, I.; Fukuyama, S.; Iwabuchi, Y.; Tanimura, R. Nat. Biotechnol. 1998, 16, 463.
13. Wagner, J.; Lerner, R. A.; Barbas, C. F., III. Science 1995, 270, 1797.
14. (a) Barbas, C. F., III; Heine, A.; Zhong, G.; Hoffmann, T.; Gramatikova, S.; Björnestedt, R.; List, B.; Anderson, J.; Stura, E. A.; Wilson, I. A.; Lerner, R. A. Science 1997, 278, 2085.
15. (b) Hoffmann, T.; Zhong, G.; List, B.; Shabat, D.; Anderson, J.; Gramatikova, S.; Lerner, R. A.; Barbas, C. F., III. J. Am. Chem. Soc. 1998, 120, 2768.
16. (c) Zhong, G.; Hoffmann, T.; Lerner, R. A.; Danishefsky, S.; Barbas, C. F., III. J. Am. Chem. Soc. 1997, 119, 8131.
17. (d) List, B.; Shabat, D.; Barbas, C. F., III; Lerner, R. A. Chem. Eur. J. 1998, 4, 881.
18. (e) Zhong, G.; Shabat, D.; List, B.; Anderson, J.; Sinha, R. A.; Lerner, R. A.; Barbas, C. F., III. Angew. Chem., Int. Ed. 1998, 110, 2609.
19. (f) List, B.; Shabat, D.; Zhong, G.; Turner, J. M.; Li, A.; Bui, T.; Anderson, J.; Lerner, R. A.; Barbas, C. F., III. J. Am. Chem. Soc. 1999, 121, 7283.
20. (g) Shabat, D.; Rader, C.; List, B.; Lerner, R. A.; Barbas, C. F., III. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 6925.
21. (h) List, B.; Lerner, R. A.; Barbas, C. F., III. Org. Lett. 1999, 1, 59.
22. (i) Shabat, D.; List, B.; Lerner, R. A.; Barbas, C. F., III. Tetrahedron Lett. 1999, 40, 1437.
23. (j) Sinha, S.; Barbas, C. F., III; Lerner, R. A. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 14603.
24. The numbering is according to Kabat et al, Kabat, E. A.; Wu, T. T.; Perry, H. M.; Gottesman, K. S.; Foeller, C. Sequences of Proteins of Immunological Interest, 5th ed.; U.S. Public Health Service, National Institute of Health: Bethesda, MD, 1991.
25. Barbas, C. F., III, Burton, D. R., Scott, J., Silverman, G., Eds. Phage Display of Proteins and Peptides: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, 2000.
26. The antibody constant regions were taken from the human anti-tetanus toxoid Fab p313. The antibody light chain of this clone was also used as the recipient of the LCDR3s of 38C2 and 33F12. Barbas, C. F., III; Kang, A. S.; Lerner, R. A.; Benkovic, S. J. Proc. Natl. Acad. Sci. U.S.A. 1991, 88, 7978.
27. Phage display approach for humanization of binding antibodies: Rader, C.; Cheresh, D.; Barbas, C. F., III. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 8910.
28. Yang, W.-P.; Green, K.; Pinz-Sweeney, S.; Briones, A. T.; Burton, D. R.; Barbas, C. F., III. J. Mol. Biol. 1995, 254, 392.
29. List, B.; Barbas, C. F., III; Lerner, R. A. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 15351.
30. 5 (per active site).
31. Tanaka, F.; Kinoshita, K.; Tanimura, R.; Fujii, I. J. Am. Chem. Soc. 1996, 118, 2332 and references therein.
32. 16
33. Denmark, S. E.; Stavenger, R. A.; Wong, K.-T.; Su, X. J. Am. Chem. Soc. 1999, 121, 4982.
34. 3CN/PBS (pH 7.4). The reaction was monitored by HPLC analysis.
35. 3CN/PBS (pH 7.4).
36. d was determined as the inhibitor concentration required to inhibit 50% of the maximal binding in the competitive ELISA using 1-BSA coated well.
37. Crameri, A.; Raillard, S. A.; Bermudez, E.; Stemmer, W. P. Nature 1998, 391, 288.
38. Tanaka, F.; Lerner, R. A.; Barbas, C. F., III. Chem. Commun. 1999, 1383.