Catalytic mechanism of α-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling

Nucleic Acids Research

Volumn 41, Issue 22, 2013, Pages 10542-10555

  Barabás, Orsolya ^a,b,c   Németh, Veronika ^a   Bodor, Andrea ^d   Perczel, András ^d   Rosta, Edina ^e   Kele, Zoltán ^f   Zagyva, Imre ^a   Szabadka, Zoltán ^d   Grolmusz, Vince I ^d   Wilmanns, Matthias ^g   Vértessy, Beáta G ^a,h  

^a INSTITUTE OF ENZYMOLOGY   (Hungary)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d EÖTVÖS LORÁND UNIVERSITY   (Hungary)

^e KING'S COLLEGE LONDON   (United Kingdom)

^f UNIVERSITY OF SZEGED   (Hungary)

^g EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^h BUDAPEST UNIVERSITY OF TECHNOLOGY AND ECONOMICS   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE; DINUCLEOSIDE PHOSPHATE; NUCLEOSIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CELL DEATH; CONTROLLED STUDY; DNA METABOLISM; DNA SYNTHESIS; ENZYME MECHANISM; ENZYME SYNTHESIS; HYDROLYSIS; MOLECULAR MECHANICS; NONHUMAN; NUCLEOPHILIC ATTACK; NUCLEOPHILICITY; PHOSPHORUS NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; QUANTUM MECHANICS; SIGNAL TRANSDUCTION; X RAY CRYSTALLOGRAPHY;

BIOCATALYSIS; CRYSTALLOGRAPHY, X-RAY; DEOXYURACIL NUCLEOTIDES; MASON-PFIZER MONKEY VIRUS; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOSPHATES; PHOSPHORUS ISOTOPES; PROTEIN CONFORMATION; PYROPHOSPHATASES;

EID: 84890398550     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkt756     Document Type: Article

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