Altered active site flexibility and a structural metal-binding site in eukaryotic dUTPase: Kinetic characterization, folding, and crystallographic studies of the homotrimeric Drosophila enzyme

Journal of Biological Chemistry

Volumn 279, Issue 17, 2004, Pages 17932-17944

  Kovári, Júlia ^a   Barabás, Orsolya ^a,b   Takács, Enikõ ^a   Békési, Angéla ^a   Dubrovay, Zsófia ^a   Pongrácz, Veronika ^a   Zagyva, Imre ^a   Imre, Timea ^c   Szabó, Pál ^c   Vértessy, Beáta G ^a,d  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b EÖTVÖS LORÁND UNIVERSITY   (Hungary)

^c CHEMICAL RESEARCH CENTER   (Hungary)

^d INSTITUTE OF ENZYMOLOGY   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

ADDITION REACTIONS; CATALYSIS; CONFORMATIONS; CRYSTALLOGRAPHY; ENZYMES; ESCHERICHIA COLI; INTERFACES (MATERIALS); REACTION KINETICS;

APOPTOSIS; METAL IONS; OLIGOMERIZATION; PROTEOLYSIS;

BIOCHEMISTRY;

ALANINE; DEOXYURIDINE PHOSPHATE; DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE; MAGNESIUM ION; METAL ION; PROLINE; RECOMBINANT PROTEIN; THYMINE;

AMINO ACID SEQUENCE; APOPTOSIS; ARTICLE; BINDING SITE; CATALYSIS; CRYSTALLOGRAPHY; DNA REPAIR; DROSOPHILA; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME SPECIFICITY; ESCHERICHIA COLI; EUKARYOTE; MELTING POINT; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FOLDING; STRUCTURE ANALYSIS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; APOPTOSIS; BINDING SITES; CALORIMETRY; CATALYSIS; CHROMATOGRAPHY, THIN LAYER; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; DNA REPAIR; DROSOPHILA MELANOGASTER; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; IONS; KINETICS; LIGHT; MAGNESIUM; MASS SPECTROMETRY; MODELS, GENETIC; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PYROPHOSPHATASES; RECOMBINANT PROTEINS; SCATTERING, RADIATION; SPECTROPHOTOMETRY; SUBSTRATE SPECIFICITY; TEMPERATURE; THERMODYNAMICS; THYMINE; TRYPSIN; X-RAYS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 2342441633     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M313643200     Document Type: Article

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