The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction

Science

Volumn 299, Issue 5615, 2003, Pages 2067-2071

  Lahiri, Sushmita D ^a   Zhang, Guofeng ^b   Dunaway Mariano, Debra ^b   Allen, Karen N ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NEW MEXICO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYST ACTIVITY; CRYSTALLIZATION; PHOSPHORUS; X RAY DIFFRACTION ANALYSIS;

HIGH-ENERGY STATES;

ENZYMES;

CARBOXYLIC ACID; GLUCOSE 1,6 BISPHOSPHATE; MAGNESIUM; PHOSPHORUS;

ENZYME; PHOSPHORUS;

ARTICLE; COVALENT BOND; CRYSTALLIZATION; ENZYME ACTIVE SITE; MOLECULAR STABILITY; PRIORITY JOURNAL; REACTION ANALYSIS; STRUCTURE ANALYSIS; X RAY DIFFRACTION;

BINDING SITES; CATALYSIS; CHEMISTRY, PHYSICAL; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; GLUCOSE-6-PHOSPHATE; GLUCOSEPHOSPHATES; LACTOCOCCUS LACTIS; LIGANDS; MAGNESIUM; PHOSPHATES; PHOSPHOGLUCOMUTASE; PHOSPHORANES; PHOSPHORUS; PHOSPHORYLATION; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY;

EID: 0242585345     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1082710     Document Type: Article

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49. Supported by a grant from NIH (grant GM16099) (to K.N.A and D.D-.M). Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science, under contract no. W-31-109-Eng-38. Use of the BioCARS Sector 14 was supported by NIH, National Center for Research Resources, under grant number RR07707. Research was carried out (in part) at the HHMI beamline X4A at the National Synchrotron Light Source, Brookhaven National Laboratory. Coordinates have been deposited in the Protein Data Bank (accession codes 1O08 and 1O03, for G1P and G6P cocrystals, respectively). We thank W. W. Cleland, G. Petsko, D. Ringe, R. Holmes, and A. Mildvan for careful reading of the manuscript, and E. Peisach for his generous help with graphics.