Multidimensional NMR Identifies the Conformational Shift Essential for Catalytic Competence in the 60-kDa Drosophila melanogaster dUTPase Trimer

Journal of Biological Chemistry

Volumn 279, Issue 17, 2004, Pages 17945-17950

  Dubrovay, Zsófia ^a   Gáspári, Zoltán ^a,b   Hunyadi Gulyás, Éva ^a   Medzihradszky, Katalin F ^c   Perczel, András ^b   Vértessy, Beáta G ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b EÖTVÖS LORÁND UNIVERSITY   (Hungary)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CONFORMATIONS; DNA; ENZYMES; NUCLEAR MAGNETIC RESONANCE; REACTION KINETICS; RESONANCE; SPECTROSCOPIC ANALYSIS; TITRATION;

CONFORMATIONAL SHIFTS; TOTAL CORRELATION SPECTROSCOPY;

BIOCHEMISTRY;

ALPHA,BETA IMINO DEOXYURIDINE TRIPHOSPHATE; DEOXYURIDINE DIPHOSPHATE; DEOXYURIDINE PHOSPHATE; DEOXYURIDINE TRIPHOSPHATE DERIVATIVE; DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE; DNA; UNCLASSIFIED DRUG; URACIL;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONFORMATIONAL TRANSITION; DROSOPHILA MELANOGASTER; ENZYME ACTIVE SITE; ENZYME KINETICS; NITROGEN NUCLEAR MAGNETIC RESONANCE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN FOLDING; PROTON NUCLEAR MAGNETIC RESONANCE;

ALLOSTERIC SITE; ANIMALS; CATALYSIS; DIMERIZATION; DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PYROPHOSPHATASES; SPECTROPHOTOMETRY; SUBSTRATE SPECIFICITY; URACIL;

DROSOPHILA MELANOGASTER; EUKARYOTA; MELANOGASTER;

EID: 2342420635     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M313644200     Document Type: Article

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