Tissue Biopsies in Diabetes Research

Clinical Diabetes Research: Methods and Techniques

Volumn , Issue , 2007, Pages 265-288

  Højlund, Kurt ^a   Gaster, Michael ^b   Beck Nielsen, Henning ^a,b,c  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b ODENSE UNIVERSITY HOSPITAL   (Denmark)

^c Danish PhD School of Molecular Metabolism   (Denmark)

Author keywords

Crude muscle extracts; Glycogen synthesis; Hematoxylin and eosin staining; Insulin signaling modulators; Open muscle biopsy; Pathophysiological abnormality and physiological conditions; Percutaneous muscle biopsy; Reactive oxygen species; Skeletal muscle biopsies; Type 2 diabetes

Indexed keywords

BIOPSY;

GLYCOGEN SYNTHESIS; HEMATOXYLIN AND EOSIN STAINING; INSULIN SIGNALING; MUSCLE BIOPSY; MUSCLE EXTRACTS; PHYSIOLOGICAL CONDITION; REACTIVE OXYGEN SPECIES; SKELETAL MUSCLE; TYPE-2 DIABETES;

MUSCLE;

EID: 84889446756     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470513095.ch14     Document Type: Chapter

References (88)

1. Abdallah BM, Beck-Nielsen H, Gaster M (2005) Increased expression of 11beta-hydroxysteroid dehydrogenase type 1 in type 2 diabetic myotubes Eur J Clin Invest 35, 627-634.
2. Adhihetty PJ, Ljubicic V, Menzies KJ, Hood DA (2005) Differential susceptibility of subsarcolemmal and intermyofibrillar mitochondria to apoptotic stimuliAm J Physiol Cell Physiol, 289, C994-C1001.
3. Al-Khalili L, Chibalin AV, Kannisto K, Zhang BB, Permert J, Holman GD, Ehrenborg E, Ding VD, Zierath JR, Krook A (2003) Insulin action in cultured human skeletal muscle cells during differentiation, assessment of cell surface GLUT4 and GLUT1 content Cell Mol Life Sci 60, 991-998.
4. Askanas V, Engel WK (1975) A new program for investigating adult human skeletal muscle grown aneurally in tissue culture Neurology 25, 58-67.
5. Beck-Nielsen H, Groop LC (1994) Metabolic and genetic characterization of prediabetic states Sequence of events leading to non-insulin-dependent diabetes mellitus J Clin Invest 94, 1714-1721.
6. Beck-Nielsen H (1998) Mechanisms of insulin resistance in non-oxidative glucose metabolism, the role of glycogen synthase J Basic Clin Physiol Pharmacol 9, 255-279.
7. Beck-Nielsen H, Vaag A, Poulsen P, Gaster M (2003) Metabolic and genetic influence on glucose metabolism in type 2 diabetic subjects-experiences from relatives and twin studies Best Pract Res Clin Endocrinol Metab 17, 445-467.
8. Bergström J (1962) Muscle electrolytes in man, determined by neutron activation analysis on needle biopsy specimens, a study in normal subjects, kidney patients, and patients with chronic diarrhoea Scand J Clin Lab Invest 14, Suppl. 68, 1-110.
9. Bergström J (1975) Percutaneous needle biopsy of skeletal muscle in physiological and clinical research Scand J Clin Lab Invest 35, 609-616.
10. Blau HM, Webster C (1981) Isolation and characterization of human muscle cells Proc Natl Acad Sci USA 78, 5623-5627.
11. Cusi K, Maezono K, Osman A, Pendergrass M, Patti ME, Pratipanawatr T, DeFronzo RA, Kahn CR, Mandarino LJ (2000) Insulin resistance differentially affects the PI 3-kinase-and MAP kinase-mediated signaling in human muscle J Clin Invest 105, 311-320.
12. Damsbo P, Hermann LS, Vaag A, Hother-Nielsen O, Beck-Nielsen H (1998) Irreversibility of the defect in glycogen synthase activity in skeletal muscle from obese patients with NIDDM treated with diet and metformin Diabetes Care 21, 1489-1494.
13. Damsbo P, Vaag A, Hother-Nielsen O, Beck-Nielsen H (1991) Reduced glycogen synthase activity in skeletal muscle from obese patients with and without type 2 (non-insulin-dependent) diabetes mellitus Diabetologia 34, 239-245.
14. Edwards R, Young A, Wiles M (1980) Needle biopsy of skeletal muscle in the diagnosis of myopathy and the clinical study of muscle function and repair N Engl J Med 302, 261-271.
15. Edwards RH, Round JM, Jones DA (1983) Needle biopsy of skeletal muscle, a review of 10 years experience Muscle Nerve 6, 676-683.
16. Evans JL, Goldfine ID, Maddux BA, Grodsky GM (2002) Oxidative stress and stress-activated signaling pathways, a unifying hypothesis of type 2 diabetes Endocr Rev 23, 599-622.
17. Gaster M, Beck-Nielsen H (2004) The reduced insulin-mediated glucose oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin-evidence from cultured myotubes Biochim Biophys Acta 1690, 85-91.
18. Gaster M, Beck-Nielsen H, Schroder HD (2001a) Proliferation conditions for human satellite cells The fractional content of satellite cells APMIS 109, 726-734.
19. Gaster M, Brusgaard K, Handberg A, Højlund K, Wojtaszewski JFP, Beck-Nielsen H (2004b) The primary defect in glycogen synthase activity is not based on increased glycogen synthase kinase-3a activity in diabetic myotubes Biochem Biophys Res Com 319, 1235-1240.
20. Gaster M, Kristensen SR, Beck-Nielsen H, Schroder HD (2001b) A cellular model system of differentiated human myotubes APMIS 109, 735-744.
21. Gaster M, Petersen I, Højlund K, Poulsen P, Beck-Nielsen H (2002) The diabetic phenotype is conserved in myotubes established from diabetic subjects, evidence for primary defects in glucose transport and glycogen synthase activity Diabetes 51, 921-927.
22. Gaster M, Poulsen P, Handberg A, Schroder HD, Beck-Nielsen H (2000) Direct evidence of fibre type-dependent GLUT-4 expression in human skeletal muscle Am J Physiol Endocrinol Metab 278, E910-E916.
23. Gaster M, Rustan AC, Aas V, Beck-Nielsen H (2004a) Reduced lipid oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin, evidence from cultured myotubes Diabetes 53, 542-548.
24. Gaster M, Rustan A, Beck-Nielsen H (2005) Differential utilization of saturated palmitate and unsat-urated oleate: Evidence from Cultured Myotubes Diabetes 54, 648-656.
25. Gaster M, Schroder HD, Handberg A, Beck-Nielsen H (2001c) The basal kinetic parameters of glycogen synthase in human myotube cultures are not affected by chronic high insulin exposure Biochim Biophys Acta 1537, 211-221.
26. Gaster M, Staehr P, Beck-Nielsen H, Schroder HD, Handberg A (2001) GLUT4 is reduced in slow muscle fibres of type 2 diabetic patients, is insulin resistance in type 2 diabetes a slow, type 1 fibre disease? Diabetes 50, 1324-1329.
27. Goldberger JH, Henry WL, Randall HT (1978) Percutaneous needle biopsy of skeletal muscle, technic and application Am J Surg 136, 410-412.
28. Greig PD, Askanazi J, Kinney JM (1985) Needle biopsy of skeletal muscle using suction Surg Gynecol Obstet 160, 466-468.
29. Grimmsmann T, Levin K, Meyer MM, Beck-Nielsen H, Klein HH (2002) Delays in insulin signaling towards glucose disposal in human skeletal muscle J Endocrinol 172, 645-651.
30. Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Moller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sorensen FB, Vesterby A (1988) and Some new, simple and efficient stereological methods and their use in pathological research and diagnosis APMIS 96, 379-394.
31. Handberg A, Vaag A, Vinten J, Beck-Nielsen H (1993) Decreased tyrosine kinase activity in partially purified insulin receptors from muscle of young, non-obese first degree relatives of patients with type 2 (non-insulin-dependent) diabetes mellitus Diabetologia 36, 668-674.
32. Handberg A, Vaag A, Damsbo P, Beck-Nielsen H, Vinten J (1990) Expression of insulin regulat-able glucose transporters in skeletal muscle from type 2 (non-insulin-dependent) diabetic patients Diabetologia 33, 625-627.
33. Hansen L, Gaster M, Oakeley EJ, Brusgaard K, Damsgaard Nielsen EM, Beck-Nielsen H, Pedersen O, Hemmings BA (2004) Expression profiling of insulin action in human myotubes, induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes Biochem Biophys Res Commun 323, 685-695.
34. He J, Watkins S, Kelley DE (2001) Skeletal muscle lipid content and oxidative enzyme activity in relation to muscle fibre type in type 2 diabetes and obesity Diabetes 50, 817-823.
35. Henry RR, Abrams L, Nikoulina S, Ciaraldi TP (1995) Insulin action and glucose metabolism in nondiabetic control and NIDDM subjects Comparison using human skeletal muscle cell cultures Diabetes 44, 936-946.
36. Henry RR, Ciaraldi TP, Abrams-Carter L, Mudaliar S, Park KS, Nikoulina SE (1996a) Glycogen synthase activity is reduced in cultured skeletal muscle cells of non-insulin-dependent diabetes mellitus subjects Biochemical and molecular mechanisms J Clin Invest 98, 1231-1236.
37. Henry RR, Ciaraldi TP, Mudaliar S, Abrams L, Nikoulina SE (1996b) Acquired defects of glycogen synthase activity in cultured human skeletal muscle cells, influence of high glucose and insulin levels Diabetes 45, 400-407.
38. Højlund K, Mustard KJ, Staehr P, Hardie DG, Beck-Nielsen H, Richter EA, Wojtaszewski JF (2004) AMPK activity and isoform protein expression are similar in muscle of obese subjects with and without type 2 diabetes Am J Physiol Endocrinol Metab 286, E239-E244.
39. Højlund K, Poulsen M, Staehr P, Brusgaard K, Beck-Nielsen H (2002) Effect of insulin on protein phosphatase 2A expression in muscle in type 2 diabetes Eur J Clin Invest 32, 918-923.
40. Højlund K, Staehr P, Hansen BF, Green KA, Hardie DG, Richter EA, Beck-Nielsen H, Wojtaszewski JF (2003) Increased phosphorylation of skeletal muscle glycogen synthase at NH2-terminal sites during physiological hyperinsulinemia in type 2 diabetes Diabetes 52, 1393-1402.
41. Højlund K, Wrzesinski K, Larsen PM, Fey SJ, Roepstorff P, Handberg A, Dela F, Vinten J, McCormack JG, Reynet C, Beck-Nielsen H (2003) Proteome analysis reveals phosphorylation of ATP synthase beta-subunit in human skeletal muscle and proteins with potential roles in type 2 diabetes J Biol Chem 278, 10436-10442.
42. Jacob S, Machann J, Rett K, Brechtel K, Volk A, Renn W, Maerker E, Matthaei S, Schick F, Claussen CD, Haring HU (1999) Association of increased intramyocellular lipid content with insulin resistance in lean nondiabetic offspring of type 2 diabetic subjects Diabetes 48, 1113-1119.
43. Johnson MA, Polgar JF, Weightman DF, Appleton D (1973) Data on the distribution of fibre types in thirty-six human muscles An autopsy study J Neurosci Res 18, 118-129.
44. Kausch C, Krutzfeldt J, Witke A, Rettig A, Bachmann O, Rett K, Matthaei S, Machicao F, Haring HU, Stumvoll M (2001) Effects of troglitazone on cellular differentiation, insulin signaling, and glucose metabolism in cultured human skeletal muscle cells Biochem Biophys Res Commun 280, 664-674.
45. Kase ET, Wensaas AJ, Aas V, Højlund K, Levin K, Thoresen GH, Beck-Nielsen H, Rustan AC, Gaster M (2005) Skeletal muscle lipid accumulation in type 2 diabetes may involve the liver X receptor pathway Diabetes 54, 1108-1115.
46. Kelley DE, He J, Menshikova EV, Ritov VB (2002) Dysfunction of mitochondria in human skeletal muscle in type 2 diabetes Diabetes 51, 2944-2950.
47. Kelley DE, Mandarino LJ (2000) Fuel selection in human skeletal muscle in insulin resistance, a reexamination Diabetes 49, 677-683.
48. Kelley DE, Simoneau JA (1994) Impaired free fatty acid utilization by skeletal muscle in non-insulin-dependent diabetes mellitus J Clin Invest 94, 2349-2356.
49. Kim YB, Kotani K, Ciaraldi TP, Henry RR, Kahn BB (2003) Insulin-stimulated protein kinase C lambda/zeta activity is reduced in skeletal muscle of humans with obesity and type 2 diabetes, reversal with weight reduction Diabetes 52, 1935-1942.
50. Korsheninnikova E, Seppala-Lindroos A, Vehkavaara S, Goto T, Virkamaki A (2002) Elevated fasting insulin concentrations associate with impaired insulin signaling in skeletal muscle of healthy subjects independent of obesity Diabetes Metab Res Rev 18, 209-216.
51. Koves TR, Noland RC, Bates AL, Henes ST, Muoio DM, Cortright RN (2005) Subsarcolemmal and intermyofibrillar mitochondria play distinct roles in regulating skeletal muscle fatty acid metabolism Am J Physiol Cell Physiol 288, C1074-1082.
52. Krebs M, Roden M (2004) Nutrient-induced insulin resistance in human skeletal muscle Curr Med Chem 11, 901-918.
53. Levin K, Daa Schroeder H, Alford FP, Beck-Nielsen H (2001) Morphometric documentation of abnormal intramyocellular fat storage and reduced glycogen in obese patients with Type II diabetes Diabetologia 44, 824-833.
54. Levin K, Hother-Nielsen O, Henriksen JE, Beck-Nielsen H (2004) Effects of troglitazone in young first-degree relatives of patients with type 2 diabetes Diabetes Care 27, 148-154.
55. Lillioja S, Young AA, Culter CL, Ivy JL, Abbott WG, Zawadzki JK, Yki-Jarvinen H, Christin L, Secomb TW, Bogardus C (1987) Skeletal muscle capillary density and fibre type are possible determinants of in vivo insulin resistance in man J Clin Invest 80, 415-424.
56. Lowell BB, Shulman GI (2005) Mitochondrial dysfunction and type 2 diabetes Science 307, 384-387.
57. Mandarino LJ, Consoli A, Jain A, Kelley DE (1996) Interaction of carbohydrate and fat fuels in human skeletal muscle, impact of obesity and NIDDM Am J Physiol 270, E463-E470.
58. Mandarino LJ, Wright KS, Verity LS, Nichols J, Bell JM, Kolterman OG, Beck-Nielsen H (1987) Effects of insulin infusion on human skeletal muscle pyruvate dehydrogenase, phosphofructokinase, and glycogen synthase Evidence for their role in oxidative and nonoxidative glucose metabolism J Clin Invest 80, 655-663.
59. Mathieu-Costello O, Kong A, Ciaraldi TP, Cui L, Ju Y, Chu N, Kim D, Mudaliar S, Henry RR (2003) Regulation of skeletal muscle morphology in type 2 diabetic subjects by troglitazone and metformin, relationship to glucose disposal Metabolism 52, 540-546.
60. Marin P, Andersson B, Krotkiewski M, Bjorntorp P (1994) Muscle fibre composition and capillary density in women and men with NIDDM Diabetes Care 17, 382-386.
61. McIntyre EA, Halse R, Yeaman SJ, Walker M (2004) Cultured muscle cells from insulin-resistant type 2 diabetes patients have impaired insulin, but normal 5-amino-4-imidazolecarboxamide riboside-stimulated, glucose uptake J Clin Endocrinol Metab 89, 3440-3448.
62. Meyer MM, Levin K, Grimmsmann T, Beck-Nielsen H, Klein HH (2002a) Insulin signalling in skeletal muscle of subjects with or without Type II-diabetes and first degree relatives of patients with the disease Diabetologia 45, 813-822.
63. Meyer MM, Levin K, Grimmsmann T, Perwitz N, Eirich A, Beck-Nielsen H, Klein HH (2002b) Trogli-tazone treatment increases protein kinase B phosphorylation in skeletal muscle of normoglycaemic subjects at risk for the development of type 2 diabetes Diabetes 51, 2691-2697.
64. Mootha VK, Lindgren CM, Eriksson KF, Subramanian A, Sihag S, Lehar J et al. (2003) PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes Nat Genet 34, 267-273.
65. Nikoulina SE, Ciaraldi TP, Carter L, Mudaliar S, Park KS, Henry RR (2001) Impaired muscle glycogen synthase in type 2 diabetes is associated with diminished phosphatidylinositol 3-kinase activation J Clin Endocrinol Metab 86, 4307-4314.
66. Nyholm B, Qu Z, Kaal A, Pedersen SB, Gravholt CH, Andersen JL, Saltin B, Schmitz O (1997) Evidence of an increased number of type IIb muscle fibres in insulin-resistant first-degree relatives of patients with NIDDM Diabetes 46, 1822-1828.
67. Ortenblad N, Mogensen M, Petersen I, Højlund K, Levin K, Sahlin K, Beck-Nielsen H, Gaster M (2005) Reduced Insulin-mediated Citrate Synthase Activity in cultured Skeletal Muscle cells from patients with Type 2 Diabetes; Evidence for an intrinsic oxidative enzyme defect Biochim Biophys Acta 1741, 206-214.
68. Patti ME, Butte AJ, Crunkhorn S, Cusi K, Berria R, Kashyap S, Miyazaki Y, Kohane I, Costello M, Saccone R, Landaker EJ, Goldfine AB, Mun E, DeFronzo R, Finlayson J, Kahn CR, Mandarino LJ (2003) Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes, Potential role of PGC1 and NRF1 Proc Natl Acad Sci USA 100, 8466-8471.
69. Petersen KF, Dufour S, Befroy D, Garcia R, Shulman GI (2004) Impaired mitochondrial activity in the insulin-resistant offspring of patients with type 2 diabetes N Engl J Med 350, 664-671.
70. Petersen KF, Dufour S, Shulman GI (2005) Decreased insulin-stimulated ATP synthesis and phosphate transport in muscle of insulin-resistant offspring of type 2 diabetic parents PLoS Med 2(233).
71. Pirola L, Johnston AM, Van Obberghen E (2004) Modulation of insulin action Diabetologia 47, 170-184.
72. Ritov VB, Menshikova EV, He J, Ferrell RE, Goodpaster BH, Kelley DE (2005) Deficiency of subsarcolemmal mitochondria in obesity and type 2 diabetes Diabetes 54, 8-14.
73. Sarabia V, Lam L, Burdett E, Leiter LA, Klip A (1992) Glucose transport in human skeletal muscle cells in culture Stimulation by insulin and metformin J Clin Invest 90, 1386-1395.
74. Schmitz-Peiffer C (2002) Protein kinase C and lipid-induced insulin resistance in skeletal muscle Ann NY Acad Sci 967, 146-157.
75. Shulman GI (2000) Cellular mechanisms of insulin resistance J Clin Invest 106, 171-176.
76. Shulman GI (2004) Unraveling the cellular mechanism of insulin resistance in humans, new insights from magnetic resonance spectroscopy Physiology (Bethesda) 19, 183-190.
77. Sreekumar R, Halvatsiotis P, Schimke JC, Nair KS (2002) Gene expression profile in skeletal muscle of type 2 diabetes and the effect of insulin treatment Diabetes 51, 1913-1920.
78. Vaag A, Alford F, Beck-Nielsen H (1996) Intracellular glucose and fat metabolism in identical twins discordant for non-insulin-dependent diabetes mellitus (NIDDM), acquired versus genetic metabolic defects? Diabet Med 13, 806-815.
79. Vaag A, Damsbo P, Hother-Nielsen O, Beck-Nielsen H (1992b) Hyperglycaemia compensates for the defects in insulin-mediated glucose metabolism and in the activation of glycogen synthase in the skeletal muscle of patients with type 2 (non-insulin-dependent) diabetes mellitus Diabetologia 35, 80-88.
80. Vaag A, Henriksen JE, Beck-Nielsen H (1992a) Decreased insulin activation of glycogen synthase in skeletal muscles in young nonobese Caucasian first-degree relatives of patients with non-insulin-dependent diabetes mellitus J Clin Invest 89, 782-788.
81. Vaag A, Skott P, Damsbo P, Gall MA, Richter EA, Beck-Nielsen H (1991) Effect of the antilipolytic nicotinic acid analogue acipimox on whole-body and skeletal muscle glucose metabolism in patients with non-insulin-dependent diabetes mellitus J Clin Invest 88, 1282-1290.
82. Virkamaki A, Korsheninnikova E, Seppala-Lindroos A, Vehkavaara S, Goto T, Halavaara J, Hakkinen AM, Yki-Jarvinen H (2001) Intramyocellular lipid is associated with resistance to in vivo insulin actions on glucose uptake, antilipolysis, and early insulin signaling pathways in human skeletal muscle Diabetes 50, 2337-2343.
83. Wells L, Vosseller K, Hart GW (2003) A role for N-acetylglucosamine as a nutrient sensor and mediator of insulin resistance Cell Mol Life Sci 60, 222-228.
84. Wojtaszewski JF, Hansen BF, Gade, Kiens B, Markuns JF, Goodyear LJ, Richter EA (2000) Insulin signaling and insulin sensitivity after exercise in human skeletal muscle Diabetes 49, 325-331.
85. Worm D, Vinten J, Staehr P, Henriksen JE, Handberg A, Beck-Nielsen H (1996) Altered basal and insulin-stimulated phosphotyrosine phosphatase (PTPase) activity in skeletal muscle from NIDDM patients compared with control subjects Diabetologia 39, 1208-1214.
86. Wright KS, Beck-Nielsen H, Kolterman OG, Mandarino LJ (1988) Decreased activation of skeletal muscle glycogen synthase by mixed-meal ingestion in NIDDM Diabetes 37, 436-440.
87. Yasin R, Van BG, Nurse KC, Al-Ani S, Landon DN, Thompson EJ (1977) A quantitative technique for growing human adult skeletal muscle in culture starting from mononucleated cells J Neurol Sci 32, 347-360.
88. Zierath JR, He L, Guma A, Odegoard Wahlstrom E, Klip A, Wallberg-Henriksson H (1996) Insulin action on glucose transport and plasma membrane GLUT4 content in skeletal muscle from patients with NIDDM Diabetologia 39, 1180-1189.