Expression level tuning for optimal heterologous protein secretion in Saccharomyces cerevisiae

Biotechnology Progress

Volumn 13, Issue 2, 1997, Pages 117-122

  Parekh, Rajesh N ^a   Wittrup, K Dane ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APROTININ; MESSENGER RNA;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CATTLE; INTRACELLULAR FLUID; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN FOLDING; SACCHAROMYCES CEREVISIAE; SECRETION;

AMINO ACID SEQUENCE; ANIMALS; APROTININ; CATTLE; INTRACELLULAR FLUID; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE;

EID: 0031104672     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp970009d     Document Type: Article

References (35)

1. Becker, D. M.; Guarente, L. Methods Enzymol. 1991, 194, 182-187.
2. Blanch, H. W.; Clark, D. S. Biochemical Engineering; Marcel Dekker, Inc.: New York, 1996; p 213.
3. Buckholz, R. G.; Gleeson, M. A. G. Yeast systems for the commercial production of heterologous proteins. Bio/Technology 1991, 9, 1067-1072.
4. Cid, V. J.; Duran, A.; Del Rey, F.; Snyder, M. P.; Nombela, C.; Sanchez, M. Molecular Basis of Cell Integrity and Morphogenesis in Saccharomyces cerevisiae. Microbiol. Rev. 1995, 59, 345-386.
5. Cregg, J. M.; Vedvick, T. S.; Raschke, W. C. Recent Advances in the Expression of Foreign Genes in Pichia pastoris. Bio/Technology 1993, 11, 905-910.
6. Creighton, T. E. Protein Folding Pathways Determined Using Bisulphide Bonds. BioEssays 1992, 14, 195-199.
7. Faber, K. N.; Warder, W.; Ab, G.; Veenhuis, M. Review: Methylotrophic Yeasts as Factories for the Production of Foreign Proteins. Yeast 1995, 11, 1331-1344.
8. Fritz, H.; Wunderer, G. Biochemistry and applications of Aprotinin, the Kallikrein inhibitor from bovine organs. Arzneim.-Forsch. 1983, 3, 479-494.
9. Goldenberg, D. P. Native and non-native intermediates in the BPTI folding pathway. TIBS 1992, 17, 257-261.
10. Guarna, M. M.; Farm, C. H.; Busby, S. J.; Waler, K. M.; Kilburn, D. G.; Piret, J. M. Effect of cDNA Copy Number on Secretion Rate of Activated Protein C. Biotechnol. Bioeng. 1995, 46, 22-27.
11. Hammond, C.; Helenius, A. Quality Control in the Secretory Pathway. Curr. Opin. Cell Biol. 1995, 7, 523-529.
12. Hann, B.; Walter, P. The signal recognition particle in S. cerevisiae. Cell 1991, 67, 131-144.
13. Heidler, S. A.; Radding, J. A. The AUR1 Gene in Saccharomyces cerevisiae Encodes Dominant Resistance to the Antifungal Agent Aureobasidin A (LY295337). Antimicrob. Agents Chemother. 1995, 39, 2765-2769.
14. Helenius, A.; Marquardt, T.; Braakman, I. The endoplasmic reticulum as a protein-folding compartment. Trends Cell Biol. 1992, 2, 227-231.
15. Hewlett, G. Apropos aprotinin: a review. Bio/Technology 1990, 8, 565-568.
16. Klis, F. M. Review: Cell Wall Assembly in Yeast. Yeast 1994, 10, 851-869.
17. Makhatadze, G. I.; Kim, K.; Woodward, C.; Privalov, P. L. Thermodynamics of BPTI folding. Protein Sci. 1993, 2, 2028-2036.
18. Parekh, R.; Forrester, K.; Wittrup, K. D. Multicopy Overexpression of Bovine Pancreatic Trypsin Inhibitor Saturates the Protein Folding and Secretory Capacity of Saccharomyces cerevisiae. Protein Exp. Purif. 1995, 6, 537-545.
19. Parekh, R. N.; Shaw, M. R.; Wittrup, K. D. An Integrating Vector for Tunable, High Copy, Stable Integration into the Dispersed Ty δ Sites of Saccharomyces cerevisiae. Biotechnol. Prog. 1996, 12, 16-21.
20. Pendse, G. J.; Karkare, S.; Bailey, J. E. Effect of Cloned Gene Dosage on Cell Growth and Hepatitis B Surface Antigen Synthesis and Secretion in Recombinant CHO Cells. Biotechnol. Bioeng. 1992, 40, 119-129.
21. Reff, M. E. High-level production of recombinant immunoglobulins in mammalian cells. Curr. Opin. Biotechnol. 1993, 4, 573-576.
22. Robert, S.; Wagner, B. K. J.; Boulanger, M.; Richer, M. Aprotinin. Ann. Pharmacother. 1996, 30, 372-380.
23. Robinson, A. S.; Wittrup, K. D. Constitutive Overexpression of Secreted Heterologous Proteins Decreases Extractable BiP and Protein Disulfide Isomerase Levels in Saccharomyces cerevisiae. Biotechnol. Prog. 1995, 11, 171-177.
24. Rocha, E.; Paramo, J. A.; Hermida, J. Aprotinine in the control of surgical hemorrhage. Medicina-clinica 1996, 106, 307-316.
25. Romanos, M. Advances in the use of Pichia pastoris for high-level gene expression. Curr. Opin. Biotechnol. 1995, 6, 527-533.
26. Romanos, M. A.; Scorer, C. A.; Clare, J. J. Foreign Gene Expression in Yeast: a Review. Yeast 1992, 8, 423-488.
27. Royston, D. Preventing the inflammatory response to open-heart surgery - the role of aprotinin and other protease inhibitors. Int. J. Cardiol. 1996, 53, S11-37.
28. Schultz, L. D.; Hofmann, K. J.; Mylin, L. M.; Montgomery, D. L.; Ellis, R. W.; Hopper, J. E. Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multicopy expression vectors in yeast. Gene 1987, 61, 123-133.
29. Simmons, L. C.; Yansura, D. G. Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli. Nat. Biotechnol. 1996, 14, 629-634.
30. St. John, T.; Davis, R. J. Mol. Biol. 1981, 152, 285-315.
31. Sudbery, P. E. The Non-Saccharomyces Yeasts. Yeast 1994, 10, 1707-1726.
32. Vedvick, T.; Buckholz, R. G.; Engel, M.; Urcan, M.; Kinney, J.; Provow, S.; Siegel, R. S.; Thill, G. P. High-level secretion of biologically active aprotinin from the yeast Pichia pastoris. J. Ind. Microbiol. 1991, 7, 197-202.
33. Weissman, J. S.; Kim, P. S. A kinetic explanation for the rearrangement pathway of BPTI folding. Nat. Struct. Biol. 1995, 2, 1123-1130.
34. Wittrup, K. D.; Robinson, A. S.; Parekh, R. N.; Forrester, K. J. Existence of an Optimum Expression Level for Secretion of Foreign Proteins in Yeast. Ann. N. Y. Acad. Sci. 1994, 745, 321-330.
35. Wurm, F. M.; Johnson, A.; Ryll, T.; Kohne, C.; Scherthan, H.; Glaab, F.; Lie, Y. S.; Petropoulos, C. J.; Arathoon, W. R. Gene Transfer and Amplification in CHO Cells - Efficient Methods for Maximizing Specific Productivity and Assessment of Genetic Consequences. Ann. N. Y. Acad. Sci. 1996, 782, 70-78.