Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae

Yeast

Volumn 15, Issue 14, 1999, Pages 1541-1553

  Goldstein, Alan L ^a   McCusker, John H ^a,b  

^a 3020 Duke University Medical Center ^*  (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Bialaphos; Dominant drug resistance marker; Functional analysis; Gene deletion; Hygromycin B; kanMX; Nourseothricin; PCR targeting; pFA plasmids; Phosphinothricin; Yeast genome analysis

Indexed keywords

BIALAPHOS; HYGROMYCIN B; KANAMYCIN; NOURSEOTHRICIN;

ANTIBIOTIC RESISTANCE; ARTICLE; GENE CASSETTE; GENE DISRUPTION; GENE LOCUS; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PHENOTYPE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

DRUG RESISTANCE, MICROBIAL; GENE DELETION; GENES, DOMINANT; HYGROMYCIN B; MUTATION; ORGANOPHOSPHORUS COMPOUNDS; SACCHAROMYCES CEREVISIAE; STREPTOTHRICINS; TRANSFORMATION, GENETIC;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0032873415     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K     Document Type: Article

References (42)

1. Amberg, D. C., Botstein, D. and Beasley, E. M. (1995). Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction. Yeast 11, 1275-1280.
2. Atkinson, K. D., Jensen, B., Kolat, A. I., Storm, E. M., Henry, S. A. and Fogel, S. (1980). Yeast mutants auxotrophic for choline or ethanolamine. J. Bact. 141, 558-564.
3. Austin, B., Hall, R. M. and Tyler, B. M. (1990). Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93, 157-162.
4. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Smith, J. A., Seidman, J. G. and Struhl, K. (eds) (1995). Current Protocols in Molecular Biology. Wiley Interscience, New York.
5. Avalos, J., Geever, R. F. and Case, M. E. (1989). Bialaphos resistance as a dominant selectable marker in Neurospora crassa. Curr. Genet. 16, 369-72.
6. Baganz, F., Hayes, A., Marren, D., Gardner, D. C. J. and Oliver, S. G. (1997). Suitability of replacement markers for functional analysis studies in Saccharomyces cerevisiae. Yeast 13, 1563-1573.
7. Ball, S. G., Wickner, R. B., Cottarel, G., Schaus, M. and Tirtiaux, C. (1986). Molecular cloning and characterization of ARO7-OSM2, a single yeast gene necessary for chorismate mutase activity and growth in hypertonic medium. Mol. Gen. Genet. 205, 326-330.
8. Brachmann, C. B., Davies, A., Cost, G. J., Caputo, E., Li, J., Hieter, P. and Boeke, J. D. (1998). Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14, 115-132.
9. Chakraborty, B. N., Patterson, N. A. and Kapoor, M. (1991). An electroporation-based system for high-efficiency transformation of germinated conidia of filamentous fungi. Can. J. Microbiol. 37, 858-863.
10. Cooper, T. G. (1982). Transport in Saccharomyces cerevisiae. In Strathern, J. N., Jones, E. W. and Broach, J. R. (Eds), The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression. Cold Spring Harbor Laboratory Press, New York, pp. 399-461.
11. Dillon, P. J. and Rosen, C. A. (1990). A rapid method for the construction of synthetic genes using the polymerase chain reaction. BioTechniques 9, 298-299.
12. Freese, E. B., Olempska-Beer, Z., Hartig, A. and Freese, E. (1984). Initiation of meiosis and sporulation of Saccharomyces cerevisiae by sulfur or guanine deprivation. Devel. Biol. 102, 438-451.
13. Gaber, R. F., Copple, D. M., Kennedy, B. K., Vidal, M. and Bard, M. (1989). The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol. Cell. Biol. 9, 3447-3456.
14. Geitz, R. D., Scheistl, R. H., Wellems, A. R. and Woods, R. A. (1995). Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11, 355-360.
15. Goldstein, A. L., Pan, X. and McCusker, J. H. (1999). Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae. Yeast 15, 507-511.
16. Gritz, L. and Davies, J. (1983). Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25, 179-188.
17. Hammond, J. R. M., Lancashire, W. D., Meaden, P. G., Oliver, S. G. and Smith, N. A. (1994). Stability of Genetically Modified Yeast in Relation to Beer of Good and Consistent Quality. MAFF (Ministry of Agriculture, Fisheries and Food), London.
18. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K. and Pease, L. R. (1989). Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59.
19. Krugel, H., Fiedler, G., Smith, C. and Baumberg, S. (1993). Sequence and transcriptional analysis of the nourseothricin acetyltransferase-encoding gene nat1 from Streptomyces noursei. Gene 127, 127-131.
20. Langle-Rouault, F. and Jacobs, E. (1995). A method for performing precise alterations in the yeast genome using a recyclable selectable marker. Nucleic Acids Res. 23, 3079-3081.
21. Lea, P. J., Joy, K. W., Ramos, J. L. and Guerrero, M. G. (1984). The action of 2-amino-4-(methyl-phosphinyl)-butanoic acid (phosphinothricin) and its 2-oxo-derivative on the metabolism of cyanobacteria and higher plants. Phytochemistry 23, 1-6.
22. McCusker, J. H., Clemons, K. V., Stevens, D. A. and Davis, R. W. (1994). Genetic characterization of pathogenic Saccharomyces cerevisiae isolates. Genetics 136, 1261-1269.
23. Mortimer, R. K., Romano, P., Suzzi, G. and Polsinelli, M. (1994). Genome renewal: a new phenomenon revealed from a genetic study of 43 strains of Saccharomyces cerevisiae derived from natural fermentation of grape musts. Yeast 10, 1543-1552.
24. Niedenthal, R. K., Riles, L., Johnston, M. and Hegemann, J. H. (1996). Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast. Yeast 12, 773-786.
25. Palmero, L. M., Leak, F. W., Tove, S. and Parks, L. W. (1997). Assessment of the essentiality of ERG genes late in ergosterol biosynthesis in Saccharomyces cerevisiae. Curr. Genet. 32, 93-99.
26. Pettinger, R. C., Wolfe, R. N., Hoehn, M. M., Marks, P. N., Dailey, W. A. and McGuire, J. M. (1953). Hygromycin I. Preliminary studies on the production and biological activity of a new antibiotic. Antibiot. Chemother. 3, 1268-1278.
27. Platt, T. (1984). Toxicity of 2-deoxygalactose to Saccharomyces cerevisiae cells constitutively synthesizing galactose metabolizing enzymes. Mol. Cell. Biol. 4, 994-996.
28. Pomper, S. and Burkholder, P. R. (1949). Studies on the biochemical genetics of yeast. Proc. Natl Acad. Sci. U S A 35, 456-464.
29. Rose, M. D., Winston, F. and Hieter, P. (1989). Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, New York.
30. Schroeder, R. and Breitenbach, M. (1981). Metabolism of myo-inositol during sporulation of myo-inositol-requiring Saccharomyces cerevisiae. J. Bacteriol. 146, 775-783.
31. Shoemaker, D. D., Lashkari, D. A., Morris, D., Mittman, M. and Davis, R. W. (1996). Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy. Nature Genet. 14, 450-456.
32. Sikorski, R. S. and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
33. Smith, V., Botstein, D. and Brown, P. O. (1995). Genetic footprinting: a genomic strategy for determining a gene's function given its sequence. Proc. Natl Acad. Sci. U S A 92, 6479-6483.
34. Smith, V., Chou, K. N., Lashkari, D., Botstein, D. and Brown, P. O. (1996). Functional analysis of the genes of yeast chromosome V by genetic footprinting. Science 274, 2069-2074.
35. Strauch, E., Wohlleben, W. and Pühler, A. (1988). Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Streptomyces lividans and Escherichia coli. Gene 63, 65-74.
36. Thompson, C. J. and Seto, H. (1995). Bialaphos. Biotechnology 28, 197-222.
37. Van den Berg, M. A. and Steensma, H. Y. (1997). Expression cassettes for formaldehyde and fluoroacetate resistance, two dominant markers in Saccharomyces cerevisiae. Yeast 13, 551-559.
38. Varma, A., Freese, E. B. and Freese, E. (1985). Partial deprivation of GTP initiates meiosis and sporulation in Saccharomyces cerevisiae. Mol. Gen. Genet. 201, 1-6.
39. Wach, A., Brachat, A., Pohlmann, R. and Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793-1808.
40. Whelan, W. L., Gocke, E. and Manney, T. R. (1979). The CAN1 locus of Saccharomyces cerevisiae: fine-structure analysis and forward mutation rates. Genetics 91, 35-51.
41. Wohlleben, W., Arnold, W., Broer, I., Hillemann, D., Strauch, E. and Pühler, A. (1988). Nucleotide sequence of the phosphinothricin-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum. Gene 70, 25-37.
42. Yocum, R. (1983). Genetic engineering of industrial yeasts. Proc. Bio. Expo. 86, 17.