Mass spectrometry for metabolic flux analysis

Biotechnology and Bioengineering

Volumn 62, Issue 6, 1999, Pages 739-750

  Wittmann, Christoph ^a   Heinzle, Elmar ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Isotopomer; Mass distribution; Mass spectrometry; Metabolic flux analysis; Metabolic modeling

Indexed keywords

CARBON; CARBOXYLATION; ENZYMES; HYDROGEN; ISOTOPES; MASS SPECTROMETRY; MATHEMATICAL MODELS; METABOLITES; NITROGEN; OXYGEN; SUBSTRATES;

FRAGMENTATION; ISOTOPOMER; METABOLIC FLUX ANALYSIS;

METABOLISM;

CARBON 13; DEUTERIUM; HYDROGEN; NITROGEN; NITROGEN 15; OXYGEN; OXYGEN 18; RADIOISOTOPE;

ACCURACY; ANALYTIC METHOD; ARTICLE; BIOTECHNOLOGY; INTERMETHOD COMPARISON; ION CURRENT; ISOTOPE LABELING; MASS FRAGMENTOGRAPHY; MASS SPECTROMETRY; METABOLITE; MODEL; NUCLEAR MAGNETIC RESONANCE; PENTOSE PHOSPHATE CYCLE; RADIOISOTOPE DISTRIBUTION; REACTION ANALYSIS; SPECTRAL SENSITIVITY;

EID: 0033586461     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990320)62:6<739::AID-BIT13>3.0.CO;2-E     Document Type: Article

References (46)

1. Bailey JE. 1991. Toward a science in metabolic engineering. Science 252: 1668-1674.
2. Bailey JE. 1998. Mathematical modeling and analysis in biochemical engineering: Past accomplishments and future opportunities. Biotechnol Prog 14:8-20.
3. Baugh J. 1993. Handbuch der Gaschromatographie. Wiesbaden: Vieweg Verlag.
4. Bonarius HPJ, Hatzimanikatis V, Meesters KPH, De Gooier CD, Schmidt G, Tramper J. 1996. Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnol Bioeng 50: 299-318.
5. Cameron DC, Chaplen FWR. 1997. Developments in metabolic engineering. Curr Opin Biotechnol 8:175-180.
6. 13C NMR studies in perfused rat hearts. J Biol Chem 258:1385-1394.
7. 13C labeled substrates. Abstract presented at the 8th European Congress on Biotechnology, Budapest.
8. 13C mass isotopomer study of anaplerotic pyruvate carboxylation in perfused rat hearts. J Biol Chem 272:26125-26131.
9. Des Rosiers C, Di Donato L, Comte B, Laplante A, Marcoux C, David F, Fernandez CA, Brunengraber H. 1995. Isotopomer analysis of citric cycle and gluconeogenesis in rat liver. J Biol Chem 270:10027-10036.
10. 13C labeling pattern of glutamate. J Biol Chem 268:4170-4180.
11. Dornseiffer P, Meyer B, Heinzle E. 1995. Modeling of anaerobic acetate kinetics in mixed biofilm culture using dynamic membrane mass spectrometric measurement. Biotechnol Bioeng 45:219-228.
12. Fell DA. 1996. Understanding the control of metabolism. London: Portland Press.
13. Ferrance JP, Goel A, Aatia MM. 1993. Utilization of glucose and amino acids in insect cultures: Quantifying the metabolic flows within the primary pathways and medium development. Biotechnol Bioeng 42: 697-707.
14. 13C mass isotopomer distributions for natural stable isotope abundance. J Mass Spectrom 31:255-262.
15. Follstad BD, Stephanopoulos G. 1998. Effect of reversible reactions on isotope label redistribution. Eur J Biochem 252:360-371.
16. Goel A, Ferrance JP, Jeong J, Aatai MM. 1993. Analysis of metabolic fluxes in batch and continuous cultures of Bacillus subtilis. Biolechnol Bioeng 42:686-696.
17. Heinzle E, Meyer B, Oezemre A, Dunn IJ. 1998. A microreactor with on-line mass spectrometry for the investigation of biological kinetics. In: Ehrfeld W, editor. Microreaction technology. Berlin: Springer, p 267-272.
18. 13C NMR investigation of the metabolism of amino acids in renal proximal convoluted tubules and streptozotocin-treated rats and rabbits. Biochem J 263:231-241.
19. Jørgensen H, Nielsen J, Villadsen J. 1995. Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations. Biotechnol Bioeng 46:117-131.
20. 13C-labeled lactate. J Biol Chem 268: 25509-25521.
21. Knapp DR. 1979. Handbook of analytical derivatization reactions. New York: John Wiley & Sons.
22. Laplante A, Comte B, Des Rosiers C. 1995. Assay of blood and tissue oxaloacetate and -ketoglutarate by isotope dilution gas chromatography-mass spectrometry. Anal Biochem 224:580-587.
23. Lee WNP, Byerley LO, Bergner EA. 1991. Mass isotopomer analysis. Biol Mass Spectrom 20:451-458.
24. Lieser KH. 1969. Einführung in die Kernehemie. Weinheim: Verlag Chemie.
25. Lin YY, Cheng WB, Wright CE. 1993. Glucose metabolism in mammalian cells as determined by mass isotopomer analysis. Anal Biochem 209: 267-273.
26. 2 molecules. Chin J Phys 30:653-663.
27. Marx A, de Graaf AA, Wiechert W, Eggeling L, Sahm H. 1996. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol Bioeng 49:111-129.
28. Meyer B, Heinzle E. 1998. Dynamic determination of anaerobic acetate kinetics using membrane mass spectrometry. Biotechnol Bioeng 57: 127-135.
29. NIST. 1990. NIST/EPA/MSDC standard spectral database. Gaithersburg, MD: National Institute of Standards and Technology.
30. Prinsen E, Van Dongen W, Esmans EL, Van Onckelen HA. 1997. HPLC linked electrospray tandem mass spectrometry: a rapid and reliable method to analyze indole-3-acetic acid metabolism in bacteria. J Mass Spectrom 32:12-22.
31. Park SM. 1996. Investigation of carbon fluxes in central carbon metabolic pathways of Corynebacterium glutamicum. PhD thesis, Massachusetts Institute of Technology.
32. 13C-NMR spectroscopy and GC-MS. Appl Microbiol Biotechnol 47: 430-440.
33. 13C]glucose incubation. Eur J Biochem 217:457-468.
34. Rizzi M, Theobald U, Querfurth E, Rohrhirsch T, Baltes M, Reuss M. 1996. In vivo investigations of glucose transport in Saccaromyces cerevisiae. Biotechnol Bioeng 49:316-327.
35. Sauer U, Hatzimanikatis V, Bailey JE, Hochull M, Szyperski T, Wüthrich K. 1997. Metabolic fluxes in riboflavin-producing Bacillus subtilis. Nat Biotechnol 15:448-452.
36. Schmidt K, Carlsen M, Nielsen J, Villadsen J. 1997. Modeling isotopomer distributions in biochemical networks using isotopomer mapping matrices. Biotechnol Bioeng 55:831-840.
37. Schmidt K, Nielsen J, Villadsen J. 1998. Quantitative analysis of metabolic fluxes in E. coli using 2 dimensional NMR spectroscopy and complete isotopomer models. J Biotechnol, in press.
38. 1H-NMR spectroscopy. Eur J Biochem 213:1325-1331.
39. 13C labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism. Eur J Biochem 232:433-448.
40. Theobald U, Mailinger W, Baltes M, Rizzi M, Reuss M. 1997. In vivo analysis of metabolic dynamics in Saccaromyces cerevisiae: I. Experimental observations. Biotechnol Bioeng 55:305-316.
41. Vallino JJ, Stephanopoulos G. 1993. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol Bioeng 41:633-646.
42. 13C NMR studies of the biosynthesis by Microbacterium ammoniaphilum of 1-glutamate selectively enriched with carbon-13. J Biol Chem 257: 1189-1195.
43. Wiechert W, de Graaf AA. 1997. Bidirectional reaction steps in metabolic networks. I. Modeling and simulation of carbon isotope labeling experiments. Biotechnol Bioeng 55:101-117.
44. Wiechert W, Siefke C, de Graaf AA, Marx A. 1997. Bidirectional reaction steps in metabolic networks. II. Estimation and statistical analysis. Biotechnol Bioeng 55:118-135.
45. Zupke C, Stephanopoulos G. 1994. Modeling of isotope distributions and intracellular fluxes in metabolic networks using atom mapping matrices. Biotechnol Prog 10:489-498.
46. 13C NMR. Biotechnol Bioeng 45:292-303.