Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins

Yeast

Volumn 23, Issue 2, 2006, Pages 83-99

  Idiris, Alimjan ^a   Bi, Kewei ^a,b   Tohda, Hideki ^a   Kumagai, Hiromichi ^a   Giga Hama, Yuko ^a  

^a ASAHI GLASS CO LTD   (Japan)

^b HEBEI UNIVERSITY   (China)

Author keywords

Disruptant; Heterologous expression; Human growth hormone; Protease; Proteolytic degradation; Schizosaccharomyces pombe

Indexed keywords

CYSTEINE PROTEINASE; GENE PRODUCT; HUMAN GROWTH HORMONE; PROTEINASE; SERINE PROTEINASE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME DEFICIENCY; ENZYME MODIFICATION; FUNGAL STRAIN; GENE DISRUPTION; GENETIC MANIPULATION; GENETIC RECOMBINATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; SCHIZOSACCHAROMYCES POMBE;

BIOTECHNOLOGY; BLOTTING, SOUTHERN; DNA, FUNGAL; HUMAN GROWTH HORMONE; MUTAGENESIS, INSERTIONAL; PEPTIDE HYDROLASES; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS;

ANIMALIA; SCHIZOSACCHAROMYCES POMBE;

EID: 33644945195     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1342     Document Type: Article

References (49)

1. Bröker M, Ragg H, Karges HE. 1987. Expression of human antithrombin III in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Biochim Biophys Acta 908: 203-213.
2. Bädhler J, Wu JQ, Longtine MS, et al. 1998. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14: 943-951.
3. Chapman GE, Rogers KM, Brittain T, et al. 1981. The 20 000 molecular weight variant of human growth hormone. Preparation and some physical and chemical properties. J Biol Chem 256: 2395-2401.
4. Chung BH, Park KS. 1998. Simple approach to reducing proteolysis during the secretory production of human parathyroid hormone in Saccharomyces cerevisiae. Biotechnol Bioeng 57: 245-249.
5. Copley KS, Alm SM, Schooley DA, Courchesne VVE. 1998. Expression, processing and secretion of a proteolytically-sensitive insect diuretic hormone by Saccharomyces cerevisiae requires the use of a yeast strain lacking genes encoding the Yap3 and Mkc7 endoproteases found in the secretory pathway. Biochem J 330: 1333-1340.
6. Davey J, Davis K, Imai Y, Yamamoto M, Matthews G. 1994. Isolation and characterisation of krp, a dibasic endopeptidase required for cell viability in the fission yeast Schizosaccharomyces pombe. EMBO J 13: 5910-5921.
7. Enfors SO. 1992. Control of in vivo proteolysis in the production of recombinant proteins. Trends Biotechnol 10: 310-315.
8. Giga-Hama Y. 1997. Fission yeast Schizosaccharomyces pombe: an attractive host for heterologous protein production. In Foreign Gene Expression in Fission Yeast Schizosaccharomyces pombe, Giga-Hama Y, Kumagai H (eds). Springer-Verlag: Berlin;, 159 3-28178.
9. Giga-Hama Y, Kumagai H. 1999. Expression system for foreign genes using the fission yeast Schizosaccharomyces pombe. Biotechnol Appl Biochem 30: 235-244.
10. Giga-Hama Y, Tohda H, Okada H, et al. 1994. High level expression of human lipocortin I in the fission yeast Schizosaccharomyces pombe using a novel expression vector. Bio/Technology 12: 400-404.
11. Gonzalez-Lopez CI, Szabo R, Blanchin-Roland S, Gaillardin C. 2002. Genetic control of extracellular protease synthesis in the yeast Yarrowia lipolytica. Genetics 160: 417-427.
12. Hiramatsu R, Yamashita T, Aikawa J, Horinouchi S, Beppu T. 1990. The prepro-peptide of Mucor rennin directs the secretion of human growth hormone by Saccharomyces cerevisiae. Appl Environ Microbiol 56: 2125-2132.
13. Hirano T, Funabashi S, Uemura T, Yanagida M. 1986. Isolation and characterization of Schizosaccharomyces pombe cut mutants that block nuclear division but not cytokinesis. EMBO J 5: 2973-2979.
14. Ikeda S, Nikaido K, Araki K, et al. 2004. Production of recombinant human lysosomal acid lipase in Schizosaccharomyces pombe: development of a fed-batch fermentation and purification process. J Biosci Bioeng 98: 366-373.
15. Imai Y, Miyake S, Hughes DA, Yamamoto M. 1991. Identification of a GTPase-activating protein homolog in Schizosaccharomyces pombe. Mol Cell Biol 11: 3088-3094.
16. + encode putative proteases involved in the mating response. Mol Cell Biol 12: 1827-1834.
17. Isoai A, Kimura H, Reichert A, et al. 2002. Production Of D-amino acid oxidase (DAO) of Trigonopsis variabilis in Schizosaccharomyces pombe and the characterization of biocatalysts, prepared with recombinant cells. Biotechnol Bioeng 80: 22-32.
18. Jones EW. 1991. Tackling the protease problem in Saccharomyces cerevisiae. Methods Enzymol 194: 429-453.
19. Jønson L, Rehfeld JF, Johnsen AH. 2004. Enhanced peptide secretion by gene disruption of CYMI, a novel protease in Saccharomyces cerevisiae. Eur J Biochem 271: 4788-4797.
20. Kang HA, Choi ES, Hong WK, et al. 2000. Proteolytic stability of recombinant human serum albumin secreted in the yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol 53: 575-582.
21. Kang HA, Kim SJ, Choi ES, Rhee SK, Chung BH. 1998. Efficient production of intact human parathyroid hormone in a Saccharomyces cerevisiae mutant deficient in yeast aspartic protease 3 (YAP3). Appl Microbiol Biotechnol 50: 187-192.
22. Käser M, Kambacheld M, Kisters-Woike B, Langer T. 2003. Oma1, a novel membrane-bound metallopeptidase in mitochondria with activities overlapping with the m-AAA protease. J Biol Chem 278: 46414-46423.
23. Ladds G, Davey J. 2000a. Sxa2 is a serine carboxypeptidase that degrades extracellular P-factor in the fission yeast Schizosaccharomyces pombe. Mol Microbiol 36: 377-390.
24. Ladds G, Davey J. 2000b. Identification of proteases with shared functions to the proprotein processing protease krp1 in the fission yeast Schizosaccharomyces pombe. Mol Microbiol 38: 839-853.
25. Lewis UJ. 1984. Variants of growth hormone and prolactin and their post-translational modifications. Annu Rev Physiol 46: 33-42.
26. Lewis UJ. 1992. Growth hormone: what is it and what does it do? Trends Endocrinol Metab 3: 117-121.
27. Lewis UJ, Dunn JT, Bonewald LF, Seavey BK, Vanderlaan WP. 1978. A naturally occurring structural variant of human growth hormone. J Biol Chem 253: 2679-2687.
28. Li CH. 1982. Human growth hormone: 1974-1981. Mol Cell Biochem 46: 31-41.
29. Meerman HJ, Georgiou G. 1994. Construction and characterization of a set of E. coli strains defficient in all known loci affecting the proteolytic stability of secreted recombinant proteins. Bio/Technology 12: 1107-1110.
30. Moehle CM, Dixon CK, Jones EW. 1989. Processing pathway for protease B of Saccharomyces cerevisiae. J Cell Biol 108: 309-325.
31. Okada H, Sekiya T, Yokoyama K, et al. 1998a. Efficient secretion of Trichoderma reesei cellobiohydrolase II in Schizosaccharomyces pombe and characterization of its products. Appl Microbiol Biotechnol 49: 301-308.
32. Okada H, Tada K, Sekiya T, et al. 1998b. Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414. Appl Environ Microbiol 64: 555-563.
33. Okazaki K, Okazaki N, Kume K, et al. 1990. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res 18: 6485-6489.
34. Park SJ, Georgiou G, Lee SY. 1999. Secretory production of recombinant protein by a high cell density culture of a protease-negative mutant Escherichia coli strain. Biotechnol Prog 15: 164-167.
35. Patra AK, Mukhopadhyay R, Mukhija R, et al. 2000. Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli. Protein Expr Purif 18: 182-192.
36. Russell P. 1989. Gene cloning and expression in fission yeast. In Molecular Biology of the Fission Yeast, Nasim A, Young P, Johnson BF (eds). Academic Press: San Diego, CA; 243-271.
37. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY.
38. Sander P, Grünewald S, Reiländer H, Michel H. 1994. Expression of the human D2S dopamine receptor in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe: a comparative study. FEBS Lett 344: 41-46.
39. Sato S, Suzuki H, Widyastuti U, Hotta Y, Tabata S. 1994. Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe. Curr Genet 26: 31-37.
40. Siegel RS, Brierley RA. 1990. Use of a cell recycle reactor to increase production of a proteolysis-susceptible peptide secreted from recombinant Saccharomyces cerevisiae. Bio/Technology 8: 639-643.
41. Spolaore B, Polverino de Laureto P, Zambonin M, Fontana A. 2004. Limited proteolysis of human growth hormone at low pH: isolation, characterization, and complementation of the two biologically relevant fragments, 1-44 and 45-191. Biochemistry 43: 6576-6586.
42. Tokunaga M, Kawamura A, Yonekyu S, Kishida M, Hishinuma F. 1993. Secretion of mouse β-amylase from fission yeast Schizosaccharomyces pombe: presence of chymostatin-sensitive protease activity in the culture medium. Yeast 9: 379-387.
43. Tommasino M, Contorni M, Scarlato V, et al. 1990. Synthesis, phosphorylation, and nuclear localization of human papillomavirus E7 protein in Schizosaccharomyces pombe. Gene 93: 265-270.
44. Turner BG, Avgerinos GC, Melnick LM, Moir DT. 1991. Optimization of pro-urokinase secretion from recombinant Saccharomyces cerevisiae. Biotechnol Bioeng 37: 869-875.
45. + gene regulates spindle pole body duplication and has homology to the budding yeast ESP1 gene. Cell 62: 913-925.
46. Wood V, Gwilliam R, Rajandream MA, et al. 2002. The genome sequence of Schizosaccharomyces pombe. Nature 415: 871-880.
47. Yamada H, Mori H, Momoi H, et al. 1994. A fission yeast gene encoding a protein that preferentially associates with curved DNA. Yeast 10: 883-894.
48. Zhao Y, Lieberman HB. 1995. Schizosaccharomyces pombe: a model for molecular studies of eukaryotic genes. DNA Cell Biol 14: 359-371.
49. Zhou XS, Zhang YX. 2002. Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastori by controlling the specific growth rate. Biotechnol Lett 24: 1449-1453.