Application of adaptive DO-stat feeding control to Pichia pastoris X33 cultures expressing a single chain antibody fragment (scFv)

Bioprocess and Biosystems Engineering

Volumn 35, Issue 9, 2012, Pages 1603-1614

  Ferreira, A R ^a   Ataide F ^a   Von Stosch, M ^b   Dias, J M L ^a   Clemente, J J ^c   Cunha, A E ^c   Oliveira, R ^a,c  

^a UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

^c INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

Author keywords

Adaptive process control; Constitutive protein expression; GAP promoter; Pichia pastoris

Indexed keywords

ADAPTIVE CONTROLLERS; ADAPTIVE PROCESS; CELL DENSITY; CONTROL PERFORMANCE; DO CONCENTRATION; DRY CELLS; FAST RESPONSE; FED-BATCH CULTURES; GAP PROMOTER; HIGH CELL DENSITY; OXYGEN TRANSFER; PICHIA PASTORIS; PROCESS PRODUCTIVITY; PROTEIN EXPRESSIONS; SINGLE-CHAIN ANTIBODIES;

ANTIBODIES; BATCH CELL CULTURE; DISSOLVED OXYGEN; GLYCEROL; PRODUCTIVITY; YEAST;

FEEDING;

DISSOLVED OXYGEN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCEROL; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ARTICLE; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME REGULATION; FED BATCH CULTURE; FED BATCH REACTOR; FUNGAL CELL; FUNGAL STRAIN; MOLECULAR DYNAMICS; NONHUMAN; OXYGEN SATURATION; PICHIA PASTORIS; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN EXPRESSION; PROTEIN FUNCTION;

ALGORITHMS; BIOREACTORS; FUNGAL PROTEINS; GENE EXPRESSION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; PICHIA; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES;

PICHIA PASTORIS;

EID: 84870355195     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-012-0751-z     Document Type: Article

References (31)

1. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22(4):249-270. doi:10.1002/yea.1208
2. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24(1):45-66. doi:10.1111/j.1574-6976.2000.tb00532.x
3. Menendez J, Valdes I, Cabrera N (2003) The ICL1 gene of Pichia pastoris, transcriptional regulation and use of its promoter. Yeast 20(13):1097-1108. doi:10.1002/yea.1028
4. Delroisse JM, Dannau M, Gilsoul JJ, El Mejdoub T, Destain J, Portetelle D, Thonart P, Haubruge E, Vandenbol M (2005) Expression of a synthetic gene encoding a Tribolium castaneum carboxylesterase in Pichia pastoris. Protein Expr Purif 42(2): 286-294. doi:10.1016/j.pep.2005.04.011
5. Goodrick JC, Xu M, Finnegan R, Schilling BM, Schiavi S, Hoppe H, Wan NC (2001) High-level expression and stabilization of recombinant human chitinase produced in a continuous constitutive Pichia pastoris expression system. Biotechnol Bioeng 74(6):492-497. doi:10.1002/bit.1140
6. Vassileva A, Chugh DA, Swaminathan S, Khanna N (2001) Expression of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris using the GAP promoter. J Biotechnol 88(1):21-35. doi:10.1016/s0168-1656(01)002541
7. Cos O, Ramon R, Montesinos JL, Valero F (2006) Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review. Microb Cell Fact 5:17. doi:10.1186/1475-2859-5-17
8. Zhang W, Sinha J, Smith LA, Inan M, Meagher MM (2005) Maximization of production of secreted recombinant proteins in Pichia pastoris fed-batch fermentation. Biotechnol Prog 21(2):386-393. doi:10.1021/bp049811n
9. Zhang WH, Bevins MA, Plantz BA, Smith LA, Meagher MM (2000) Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol Bioeng 70(1):1-8. doi:10.1002/1097-0290(20001005)70:1\1:: aidbit1[ 3.0.co;2-y
10. Sinha J, Plantz BA, Zhang WH, Gouthro M, Schlegel V, Liu CP, Meagher MM (2003) Improved production of recombinant ovine interferon-tau by Mut(?) strain of Pichia pastoris using an optimized methanol feed profile. Biotechnol Prog 19(3):794-802. doi:10.1021/bp025744q
11. Potgieter TI, Kersey SD, Mallem MR, Nylen AC, d'Anjou M (2010) Antibody expression kinetics in glycoengineered Pichia Pastoris. Biotechnol Bioeng 106(6):918-927. doi:10.1002/bit. 22756
12. Kobayashi K, Kuwae S, Ohya T, Ohda T, Ohyama M, Tomomitsu K (2000) High level secretion of recombinant human serum albumin by fed-batch fermentation of the methylotrophic yeast, Pichia pastoris, based on optimal methanol feeding strategy. J Biosci Bioeng 90(3):280-288. doi:10.1263/jbb.90.280
13. Hohenblum H, Borth N, Mattanovich D (2003) Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J Biotechnol 102(3):281-290. doi:10.1016/s0168-1656(03)00049-x
14. Cunha AE, Clemente JJ, Gomes R, Pinto F, Thomaz M, Miranda S, Pinto R, Moosmayer D, Donner P, Carrondo MJT (2004) Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng 86(4):458-467. doi:10.1002/bit.20051
15. Pais JM, Varas L, Valdes J, Cabello C, Rodriguez L, Mansur M (2003) Modeling of mini-proinsulin production in Pichia pastoris using the AOX promoter. Biotechnol Lett 25(3):251-255. doi: 10.1023/a:1022398917213
16. Ohya T, Ohyama M, Kobayashi K (2005) Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation. Biotechnol Bioeng 90(7):876-887. doi:10.1002/bit.20507
17. Plantz BA, Sinha J, Villarete L, Nickerson KW, Schlegel VL (2006) Pichia pastoris fermentation optimization: energy state and testing a growth-associated model. Appl Microbiol Biotechnol 72(2):297-305. doi:10.1007/s00253-005-02718
18. Jungo C, Rerat C, Marison IW, von Stockar U (2006) Quantitative characterization of the regulation of the synthesis of alcohol oxidase and of the expression of recombinant avidin in a Pichia pastoris Mut(?) strain. Enzyme Microb Technol 39(4):936-944. doi:10.1016/j.enzmictec.2006.01.027
19. Pal Y, Khushoo A, Mukherjee KJ (2006) Process optimization of constitutive human granulocyte-macrophage colony-stimulating factor (hGM-CSF) expression in Pichia pastoris fed-batch culture. Appl Microbiol Biotechnol 69(6):650-657. doi:10.1007/s00253-005-00186
20. Khasa YP, Khushoo A, Srivastava L, Mukherjee KJ (2007) Kinetic studies of constitutive human granulocyte-macrophage colony stimulating factor (hGM-CSF) expression in continuous culture of Pichia pastoris. Biotechnol Lett 29(12):1903-1908. doi:10.1007/s10529-007-94738
21. Berdichevsky M, d'Anjou M, Mallem MR, Shaikh SS, Potgieter TI (2011) Improved production of monoclonal antibodies through oxygen-limited cultivation of glycoengineered yeast. J Biotechnol 155(2):217-224. doi:10.1016/j.jbiotec. 2011.06.021
22. Lee CY, Lee SJ, Jung KH, Katoh S, Lee EK (2003) High dissolved oxygen tension enhances heterologous protein expression by recombinant Pichia pastoris. Process Biochem 38(8):1147-1154. doi:10.1016/s0032-9592(02)002807
23. Trentmann O, Khatri NK, Hoffmann F (2004) Reduced oxygen supply increases process stability and product yield with recombinant Pichia pastoris. Biotechnol Prog 20(6):1766-1775. doi:10.1021/bp049711h
24. Baumann K, Maurer M, Dragosits M, Cos O, Ferrer P, Mattanovich D (2008) Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins. Biotechnol Bioeng 100(1):177-183. doi: 10.1002/bit.21763
25. Charoenrat T, Ketudat-Cairns M, Stendahl-Andersen H, Jahic M, Enfors SO (2005) Oxygen-limited fed-batch process: an alternative control for Pichia pastoris recombinant protein processes. Bioprocess Biosyst Eng 27(6):399-406. doi:10.1007/s00449-005-00054
26. Oliveira R, Clemente JJ, Cunha AE, Carrondo MJT (2005) Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation. J Biotechnol 116(1):35-50. doi:10. 1016/j.jbiotec.2004.09.016
27. Lim HK, Choi SJ, Kim KY, Jung KH (2003) Dissolved-oxygenstat controlling two variables for methanol induction of rGuamerin in Pichia pastoris and its application to repeated fed-batch. Appl Microbiol Biotechnol 62(4):342-348. doi:10.1007/s00253-003-13076
28. Chung JD (2000) Design of metabolic feed controllers: application to high-density fermentations of Pichia pastoris. Biotechnol Bioeng 68(3):298-307. doi:10.1002/(sici)1097-0290(20000505) 68:3\298:aid-bit8[3.0.co;2-k
29. Narendra KS, Annaswamy AM (1989) Stable adaptive systems. Prentice-Hall, New Jersey
30. Jahic M, Rotticci-Mulder JC, Martinelle M, Hult K, Enfors SO (2002) Modeling of growth and energy metabolism of Pichia pastoris producing a fusion protein. Bioprocess Biosyst Eng 24(6):385-393. doi:10.1007/s00449-001-02745
31. Jahic M, Gustavsson M, Jansen AK, Martinelle M, Enfors SO (2003) Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J Biotechnol 102(1):45-53. doi:10.1016/s0168-1656(03)00003-8