Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter

Biotechnology and Bioengineering

Volumn 91, Issue 6, 2005, Pages 760-767

  Resina, David ^a   Cos, Oriol ^a   Ferrer, Pau ^a   Valero, Francisco ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Fed batch cultivation; Formaldehyde dehydrogenase promoter; Pichia pastoris; Rhizopus oryzae lipase

Indexed keywords

CELLS; CONCENTRATION (PROCESS); CULTIVATION; FERMENTATION; GENES; MICROORGANISMS; NITROGEN; PROTEINS;

FED-BATCH CULTIVATION; FORMALDEHYDE DEHYDROGENASE PROMOTER; PICHIA PASTORIS; RHIZOPUS ORYZAE LIPASE;

CELL CULTURE;

FORMALDEHYDE DEHYDROGENASE; METHYLAMINE; NITROGEN; SORBITOL;

ARTICLE; CARBON SOURCE; CELL DENSITY; CELL GROWTH; CONTROLLED STUDY; FED BATCH CULTURE; FED BATCH FERMENTATION; NONHUMAN; PICHIA PASTORIS;

ALDEHYDE OXIDOREDUCTASES; CELL PROLIFERATION; CULTURE MEDIA; GENE EXPRESSION REGULATION, FUNGAL; INDUSTRIAL MICROBIOLOGY; LIPASE; METHANOL; METHYLAMINES; NITROGEN; PICHIA; PROMOTER REGIONS (GENETICS); QUATERNARY AMMONIUM COMPOUNDS; RECOMBINANT PROTEINS; SORBITOL;

CELLS; CONCENTRATION; CULTIVATION; FERMENTATION; GENES; MICROORGANISMS; NITROGEN; PROTEINS;

PICHIA PASTORIS; RHIZOPUS ORYZAE;

EID: 24644465009     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20545     Document Type: Article

References (24)

1. Chung JD. 2000. Design of metabolic feed controllers: Application to high-density fermentations of Pichia pastoris. Biotechnol Bioeng 82:298-307.
2. Cos O, Serrano A, Montesinos JL, Ferrer P, Cregg JM, Valero F. 2005. Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures. J Biotechnol 116:321-335.
3. Cuvers S, Brixius P, Klauser T, Thömmes J, Weuster-Botz D, Takors R, Wandrey C. 2001. Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing Part1. Fermentation. Biotechnol Prog 17:495-502.
4. d'Anjou MC, Daugulis AJ. 2001. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol Bioeng 72:1-11.
5. Harder W, Veenhuis M. 1989. Metabolism of one-carbon compounds. In: Rose AH, Harrison JS, editors. The yeasts, Vol. 3 London: Academic Press, pp 289-316.
6. Heinemann U, Bussow K, Müller U, Umbach P. 2003. Facilites and methods for the high-throughput crystal structural analysis of human proteins. Acc Chem Res 36:157-163.
7. Hohenblum H, Gasser B, Maurer M, Borth N, Mattanovich D. 2004. Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol Bioeng 85:367-375.
8. Jahic M, Gustavsson M, Jansen AK, Martinelle M, Enfors SO. 2003. Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch process. J Biotechnol 102:45-53.
9. 31P nuclear magnetic resonance study. J Bacteriol 173:4959-4969.
10. Kobayashi K, Kuwae S, Ohya T, Ohda T, Ohyama M, Ohi H, Tomomitsu K, Ohmura T. 2000. High-level expression of recombinant human serum albumin from the methylotrophic yeast Pichia pastoris with minimal protease production and activation. J Biosci Bioeng 89:55-61.
11. Lin Cereghino J, Cregg JM. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45-66.
12. Pakula TM, Laxell M, Huuskonen A, Uusitalo J, Saloheimo M, Penttilä M. 2003. The effects of drugs inhibiting protein secretion in the filamentous fungus Trichoderma reesei. Evidence for down-regulation of genes that encode secreted proteins in the stressed cells. J Biol Chem 278: 45011-45020.
13. Prinz B, Schultchen J, Rydzewski R, Holz C, Boettner M, Stahl U, Lang C. 2004. Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics. J Struct Funct Genomics 5:29-44.
14. Resina D, Serrano A, Valero F, Ferrer P. 2004. Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. J Biotechnol 109:103-113.
15. Rodríguez-Jiménez E, Sánchez K, Roca H, Delgado JM. 1997. Different methanol feeding strategies to recombinant Pichia pastoris cultures producing high level of dextranase. Biotechnol Tech 11:461-466.
16. Shen S, Sulter G, Jeffries T, Cregg JM. 1998. A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris. Gene 216:93-102.
17. + strain of Pichia pastoris using an optimized methanol feed profile. Biotechnol Prog 19:794-802.
18. Thorpe ED, d'Anjou MC, Daugulis AJ. 1999. Sorbitol as a non-repressing carbon source for fed-batch fermentation of recombinant Pichia pastoris. Biotechnol Lett 21:669-672.
19. Trinh LB, Phue JN, Shiloach J. 2003. Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris. Biotechnol Bioeng 82:438-444.
20. Valkonen M, Penttila M, Saloheimo M. 2003. Effects of inactivation and constitutive expression of the unfolded protein response pathway on protein production in the yeast Saccharomyces cerevisiae. Appl Environ Microbiol 69:2065-2072.
21. Waterham HR, Digan ME, Koutz PJ, Lair SV, Cregg JM. 1997. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene 20:37-44.
22. Yokoyama S. 2003. Protein expression systems for structural genomics and proteomics. Curr Opin Chem Biol 7:39-43.
23. Zhang W, Bevins MA, Plantz BA, Smith LA, Meagher MM. 2000. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol Bioeng 70:1-8.
24. Zhang W, Smith LA, Plantz BA, Schlegel VL, Meagher MM. 2002. Design of methanol feed control in Pichia pastoris fermentations based upon a growth model. Biotechnol Prog 18:1392-1399.