Resilient immortals, characterizing and utilizing Bax/Bak deficient Chinese hamster ovary (CHO) cells for high titer antibody production

Biotechnology Progress

Volumn 29, Issue 3, 2013, Pages 727-737

  Misaghi, Shahram ^a   Qu, Yan ^a   Snowden, Andrew ^a,b   Chang, Jennifer ^a   Snedecor, Brad ^a  

^a GENENTECH INC   (United States)

^b AMGEN INC   (United States)

Author keywords

Apoptosis; Bak; Bax; Chinese hamster ovary; Dihydrofolate reductase

Indexed keywords

ANTIBODY PRODUCTION; BAK; BAX; CHINESE HAMSTER OVARY; CHINESE HAMSTER OVARY CELLS; DIHYDROFOLATE REDUCTASE; LARGE-SCALE MANUFACTURING; THERAPEUTIC PROTEIN;

ANTIBODIES; CELL CULTURE; CELL DEATH;

CLONE CELLS;

ANTIBODY; BUTYRIC ACID; DIHYDROFOLATE REDUCTASE; METHOTREXATE; PROTEIN BAK; PROTEIN BAX; PUROMYCIN; RECOMBINANT PROTEIN;

ANIMAL; APOPTOSIS; ARTICLE; CHO CELL; CRICETULUS; GENE INACTIVATION; GENETICS; HAMSTER; METABOLISM; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION;

ANIMALS; ANTIBODIES; APOPTOSIS; BCL-2 HOMOLOGOUS ANTAGONIST-KILLER PROTEIN; BCL-2-ASSOCIATED X PROTEIN; BUTYRIC ACID; CHO CELLS; CRICETINAE; CRICETULUS; GENE KNOCKOUT TECHNIQUES; MEMBRANE POTENTIAL, MITOCHONDRIAL; METHOTREXATE; MITOCHONDRIA; PUROMYCIN; RECOMBINANT PROTEINS; TETRAHYDROFOLATE DEHYDROGENASE;

CRICETULUS GRISEUS;

EID: 84879259489     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1722     Document Type: Article

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