Enhancement of DNA uptake in FUT8-deleted CHO cells for transient production of afucosylated antibodies

Biotechnology and Bioengineering

Volumn 106, Issue 5, 2010, Pages 751-763

  Wong, Athena W ^a   Baginski, Tomasz K ^a   Reilly, Dorothea E ^a  

^a GENENTECH INC   (United States)

Author keywords

Afucosylation; FUT8; Heparan sulfate; Transient transfection

Indexed keywords

CELL CULTURE; CELL MEMBRANES; COMPLEXATION; DNA; GENES; LIPOSOMES; MAMMALS; NUCLEIC ACIDS; PHOSPHOLIPIDS; ZINC;

ABSENCE OF CELLS; ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITIES; CATIONIC LIPOSOMES; CATIONIC POLYMERS; CELL LINES; CELL SURFACES; CELL-SURFACE HEPARAN; CHAIN ELONGATION; CHO CELL; FC REGION; GLYCANS; HEPARAN SULFATES; HOST CELLS; HPLC ANALYSIS; MAMMALIAN CELLS; POLYETHYLENIMINES; RECOMBINANT PROTEIN; SHAKE FLASKS; TRANSIENT TRANSFECTION; WT CELLS; ZINC FINGER NUCLEASE;

ANTIBODIES;

ANTIBODY; DNA; EXOSTOSIN 1; FUCOSYLTRANSFERASE 8; HEPARIN; LIPOSOME; POLYETHYLENEIMINE; POLYMER; PROTEINASE; TRANSFERASE; UNCLASSIFIED DRUG; FUCOSYLTRANSFERASE; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CHO CELL; NONHUMAN; TRANSIENT TRANSFECTION; ANIMAL; BIOSYNTHESIS; CHEMISTRY; CHO CELL LINE; CRICETULUS; CULTURE TECHNIQUE; DEFICIENCY; GENE EXPRESSION; GENE INACTIVATION; GENETIC TRANSFECTION; GENETICS; HAMSTER; METABOLISM;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; DNA; FUCOSYLTRANSFERASES; GENE EXPRESSION; GENE KNOCKOUT TECHNIQUES; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 77955375562     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22749     Document Type: Article

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