메뉴 건너뛰기




Volumn 65, Issue 2, 2013, Pages 263-271

PEI/DNA formation affects transient gene expression in suspension Chinese hamster ovary cells via a one-step transfection process

Author keywords

CHO cells; One step transfection; Polyethylenimine (PEI); Transient gene expression

Indexed keywords

DNA; GREEN FLUORESCENT PROTEIN; POLYETHYLENEIMINE;

EID: 84878364800     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-012-9483-9     Document Type: Article
Times cited : (37)

References (28)
  • 1
    • 19744372388 scopus 로고    scopus 로고
    • Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis
    • 10.1002/jgm.696 1:CAS:528:DC%2BD2MXltlGjsL8%3D
    • Akinc A, Thomas M, Klibanov AM, Langer R (2005) Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis. J Gene Med 7:657-663
    • (2005) J Gene Med , vol.7 , pp. 657-663
    • Akinc, A.1    Thomas, M.2    Klibanov, A.M.3    Langer, R.4
  • 2
    • 38449121649 scopus 로고    scopus 로고
    • High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI
    • 10.1002/bit.21596 1:CAS:528:DC%2BD1cXns1Witg%3D%3D
    • Backliwal G, Hildinger M, Hasija V, Wurm FM (2008) High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol Bioeng 99:721-727
    • (2008) Biotechnol Bioeng , vol.99 , pp. 721-727
    • Backliwal, G.1    Hildinger, M.2    Hasija, V.3    Wurm, F.M.4
  • 3
    • 0030786458 scopus 로고    scopus 로고
    • Delivery of bacterial artificial chromosomes into mammalian cells with psoralen-inactivated adenovirus carrier
    • 10.1093/nar/25.10.1950 1:CAS:528:DyaK2sXjsFGjt7g%3D
    • Baker A, Cotten M (1997) Delivery of bacterial artificial chromosomes into mammalian cells with psoralen-inactivated adenovirus carrier. Nucleic Acids Res 25:1950-1956
    • (1997) Nucleic Acids Res , vol.25 , pp. 1950-1956
    • Baker, A.1    Cotten, M.2
  • 4
    • 0030768650 scopus 로고    scopus 로고
    • Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery
    • 10.1038/sj.gt.3300471 1:CAS:528:DyaK2sXltVaqtrc%3D
    • Baker A, Saltik M, Lehrmann H, Killisch I, Mautner V, Lamm G, Christofori G, Cotten M (1997) Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. Gene Ther 4:773
    • (1997) Gene Ther , vol.4 , pp. 773
    • Baker, A.1    Saltik, M.2    Lehrmann, H.3    Killisch, I.4    Mautner, V.5    Lamm, G.6    Christofori, G.7    Cotten, M.8
  • 5
    • 34147125994 scopus 로고    scopus 로고
    • Recombinant protein production by large-scale transient gene expression in mammalian cells: State of the art and future perspectives
    • 10.1007/s10529-006-9297-y 1:CAS:528:DC%2BD2sXjvFagu7s%3D
    • Baldi L, Hacker DL, Adam M, Wurm FM (2007) Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives. Biotechnol Lett 29:677-684
    • (2007) Biotechnol Lett , vol.29 , pp. 677-684
    • Baldi, L.1    Hacker, D.L.2    Adam, M.3    Wurm, F.M.4
  • 6
    • 78650476432 scopus 로고    scopus 로고
    • The proton sponge: A trick to enter cells the viruses did not exploit
    • 1:CAS:528:DyaK2sXhvFWkur4%3D
    • Behr JP (1997) The proton sponge: a trick to enter cells the viruses did not exploit. CHIMIA Int J Chem 51:34-36
    • (1997) CHIMIA Int J Chem , vol.51 , pp. 34-36
    • Behr, J.P.1
  • 7
    • 33751242770 scopus 로고    scopus 로고
    • Disassembly of polyethylenimine-DNA particles in vitro: Implications for polyethylenimine-mediated DNA delivery
    • 10.1016/j.jconrel.2006.09.006 1:CAS:528:DC%2BD28Xht1ems77L
    • Bertschinger M, Backliwal G, Schertenleib A, Jordan M, Hacker DL, Wurm FM (2006) Disassembly of polyethylenimine-DNA particles in vitro: Implications for polyethylenimine-mediated DNA delivery. J Controlled Release 116:96-104
    • (2006) J Controlled Release , vol.116 , pp. 96-104
    • Bertschinger, M.1    Backliwal, G.2    Schertenleib, A.3    Jordan, M.4    Hacker, D.L.5    Wurm, F.M.6
  • 8
    • 0029160083 scopus 로고
    • A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
    • 10.1073/pnas.92.16.7297 1:CAS:528:DyaK2MXntlKmt7s%3D
    • Boussif O, Lezoualc'h F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Nat Acad Sci 92:7297-7301
    • (1995) Proc Nat Acad Sci , vol.92 , pp. 7297-7301
    • Boussif, O.1    Lezoualc'H, F.2    Zanta, M.A.3    Mergny, M.D.4    Scherman, D.5    Demeneix, B.6    Behr, J.P.7
  • 9
    • 0033166095 scopus 로고    scopus 로고
    • In vivo delivery to tumors of DNA complexed with linear polyethylenimine
    • 10.1089/10430349950017662 1:CAS:528:DyaK1MXksVejtrw%3D
    • Coll JL, Chollet P, Brambilla E, Desplanques D, Behr JP, Favrot M (1999) In vivo delivery to tumors of DNA complexed with linear polyethylenimine. Hum Gene Ther 10:1659-1666
    • (1999) Hum Gene Ther , vol.10 , pp. 1659-1666
    • Coll, J.L.1    Chollet, P.2    Brambilla, E.3    Desplanques, D.4    Behr, J.P.5    Favrot, M.6
  • 12
    • 0030757526 scopus 로고    scopus 로고
    • Nanoscopic structure of DNA condensed for gene delivery
    • 10.1093/nar/25.15.3095 1:CAS:528:DyaK2sXltlCrsr4%3D
    • Dunlap DD, Maggi A, Soria MR, Monaco L (1997) Nanoscopic structure of DNA condensed for gene delivery. Nucleic Acids Res 25:3095
    • (1997) Nucleic Acids Res , vol.25 , pp. 3095
    • Dunlap, D.D.1    Maggi, A.2    Soria, M.R.3    Monaco, L.4
  • 13
    • 0038004448 scopus 로고    scopus 로고
    • A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: Effect of molecular weight on transfection efficiency and cytotoxicity
    • 10.1023/A:1014861900478 1:CAS:528:DyaK1MXlsVyntb4%3D
    • Fischer D, Bieber T, Li Y, Elsässer HP, Kissel T (1999) A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity. Pharm Res 16:1273-1279
    • (1999) Pharm Res , vol.16 , pp. 1273-1279
    • Fischer, D.1    Bieber, T.2    Li, Y.3    Elsässer, H.P.4    Kissel, T.5
  • 15
    • 0032811354 scopus 로고    scopus 로고
    • Poly (ethylenimine) and its role in gene delivery
    • 10.1016/S0168-3659(99)00090-5 1:CAS:528:DyaK1MXks1Cqu7s%3D
    • Godbey W, Wu KK, Mikos AG (1999) Poly (ethylenimine) and its role in gene delivery. J Controlled Release 60:149-160
    • (1999) J Controlled Release , vol.60 , pp. 149-160
    • Godbey, W.1    Wu, K.K.2    Mikos, A.G.3
  • 16
    • 34248146458 scopus 로고    scopus 로고
    • Transient expression of osteopontin in HEK 293 cells in serum-free culture
    • 10.1016/j.enzmictec.2006.12.013 1:CAS:528:DC%2BD2sXlt1alu74%3D
    • Han X, Sun L, Fang Q, Li D, Gong X, Wu Y, Yang S, Shen BQ (2007) Transient expression of osteopontin in HEK 293 cells in serum-free culture. Enzyme Microb Technol 41:133-140
    • (2007) Enzyme Microb Technol , vol.41 , pp. 133-140
    • Han, X.1    Sun, L.2    Fang, Q.3    Li, D.4    Gong, X.5    Wu, Y.6    Yang, S.7    Shen, B.Q.8
  • 17
    • 71349083803 scopus 로고    scopus 로고
    • The heterogeneous nature of polyethylenimine-DNA complex formation affects transient gene expression
    • 10.1007/s10616-009-9215-y 1:CAS:528:DC%2BD1MXhsVKnsbfI
    • Han X, Fang Q, Yao F, Wang X, Wang J, Yang S, Shen BQ (2009) The heterogeneous nature of polyethylenimine-DNA complex formation affects transient gene expression. Cytotechnology 60:63-75
    • (2009) Cytotechnology , vol.60 , pp. 63-75
    • Han, X.1    Fang, Q.2    Yao, F.3    Wang, X.4    Wang, J.5    Yang, S.6    Shen, B.Q.7
  • 18
    • 79960860841 scopus 로고    scopus 로고
    • Mechanisms of polyethylenimine-mediated DNA delivery: Free carrier helps to overcome the barrier of cell-surface glycosaminoglycans
    • 10.1002/jgm.1587
    • Hanzlíková M, Ruponen M, Galli E, Raasmaja A, Aseyev V, Tenhu H, Urtti A, Yliperttula M (2011) Mechanisms of polyethylenimine-mediated DNA delivery: free carrier helps to overcome the barrier of cell-surface glycosaminoglycans. J Gene Med 13:402-409
    • (2011) J Gene Med , vol.13 , pp. 402-409
    • Hanzlíková, M.1    Ruponen, M.2    Galli, E.3    Raasmaja, A.4    Aseyev, V.5    Tenhu, H.6    Urtti, A.7    Yliperttula, M.8
  • 19
    • 0029991350 scopus 로고    scopus 로고
    • Transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation
    • 10.1093/nar/24.4.596 1:CAS:528:DyaK28XhsVSmtrw%3D
    • Jordan M, Schallhorn A, Wurm FM (1996) Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic Acids Res 24:596-601
    • (1996) Nucleic Acids Res , vol.24 , pp. 596-601
    • Jordan, M.1    Schallhorn, A.2    Wurm, F.M.3
  • 20
    • 0035286969 scopus 로고    scopus 로고
    • Polyethylenimine-mediated gene delivery: A mechanistic study
    • 10.1002/jgm.173 1:STN:280:DC%2BD3MvkslGksQ%3D%3D
    • Kichler A, Leborgne C, Coeytaux E, Danos O (2001) Polyethylenimine- mediated gene delivery: a mechanistic study. J Gene Med 3:135-144
    • (2001) J Gene Med , vol.3 , pp. 135-144
    • Kichler, A.1    Leborgne, C.2    Coeytaux, E.3    Danos, O.4
  • 21
    • 0035980982 scopus 로고    scopus 로고
    • Design and gene delivery activity of modified polyethylenimines
    • 10.1016/S0169-409X(01)00202-2 1:CAS:528:DC%2BD3MXovFyrtbk%3D
    • Kircheis R, Wightman L, Wagner E (2001) Design and gene delivery activity of modified polyethylenimines. Adv Drug Deliv Rev 53:341-358
    • (2001) Adv Drug Deliv Rev , vol.53 , pp. 341-358
    • Kircheis, R.1    Wightman, L.2    Wagner, E.3
  • 23
    • 0030799944 scopus 로고    scopus 로고
    • Adenovirus enhancement of polyethylenimine-mediated transfer of regulated genes in differentiated cells
    • 10.1038/sj.gt.3300450 1:CAS:528:DyaK2sXltVeisrs%3D
    • Meunier-Durmort C, Grimal H, Sachs L, Demeneix B, Forest C (1997) Adenovirus enhancement of polyethylenimine-mediated transfer of regulated genes in differentiated cells. Gene Ther 4:808
    • (1997) Gene Ther , vol.4 , pp. 808
    • Meunier-Durmort, C.1    Grimal, H.2    Sachs, L.3    Demeneix, B.4    Forest, C.5
  • 24
    • 0026327132 scopus 로고
    • Direct observation of the biphasic conformational change of DNA induced by cationic polymers
    • 10.1016/0014-5793(91)81386-M 1:CAS:528:DyaK38Xltlyrsg%3D%3D
    • Minagawa K, Matsuzawa Y, Yoshikawa K, Matsumoto M, Doi M (1991) Direct observation of the biphasic conformational change of DNA induced by cationic polymers. FEBS Lett 295:67-69
    • (1991) FEBS Lett , vol.295 , pp. 67-69
    • Minagawa, K.1    Matsuzawa, Y.2    Yoshikawa, K.3    Matsumoto, M.4    Doi, M.5
  • 26
    • 0032953248 scopus 로고    scopus 로고
    • Transient gene expression in mammalian cells grown in serum-free suspension culture
    • 10.1023/A:1008000327766 1:CAS:528:DyaK1MXjsl2lu7c%3D
    • Schlaeger EJ, Christensen K (1999) Transient gene expression in mammalian cells grown in serum-free suspension culture. Cytotechnology 30:71-83
    • (1999) Cytotechnology , vol.30 , pp. 71-83
    • Schlaeger, E.J.1    Christensen, K.2
  • 27
    • 0030775528 scopus 로고    scopus 로고
    • The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes
    • 10.1038/sj.gt.3300454 1:CAS:528:DyaK2sXltVeiur0%3D
    • Tang M, Szoka F (1997) The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther 4:823
    • (1997) Gene Ther , vol.4 , Issue.8 , pp. 823
    • Tang, M.1    Szoka, F.2
  • 28
    • 0033039916 scopus 로고    scopus 로고
    • Large-scale transient expression in mammalian cells for recombinant protein production
    • 10.1016/S0958-1669(99)80027-5 1:CAS:528:DyaK1MXisVeltb8%3D
    • Wurm F, Bernard A (1999) Large-scale transient expression in mammalian cells for recombinant protein production. Curr Opin Biotechnol 10:156-159
    • (1999) Curr Opin Biotechnol , vol.10 , pp. 156-159
    • Wurm, F.1    Bernard, A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.