Animal-cell damage in sparged bioreactors

Trends in Biotechnology

Volumn 18, Issue 10, 2000, Pages 420-432

  Chisti, Yusuf ^a  

^a UNIVERSITY OF ALMERÍA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULOSE DERIVATIVE; DEXTRAN; HETASTARCH; MACROGOL; METHYLCELLULOSE; PLASMA PROTEIN; POLOXAMER; POLYVINYL ALCOHOL; POVIDONE; STARCH;

ANIMAL CELL; BIOREACTOR; CELL CULTURE; CELL DAMAGE; CELL PROTECTION; CULTURE MEDIUM; ENERGY; GAS; NONHUMAN; OXYGEN SUPPLY; OXYGENATION; PRIORITY JOURNAL; REVIEW; SERUM; TECHNIQUE;

ANIMALS; BIOREACTORS; BLOOD; CELL CULTURE TECHNIQUES; CULTURE MEDIA; KINETICS; MODELS, THEORETICAL; OXYGEN; POLOXAMER; POLYETHYLENE GLYCOLS; SURFACE-ACTIVE AGENTS;

ANIMALIA;

EID: 0034308263     PISSN: 01677799     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7799(00)01474-8     Document Type: Review

References (89)

1. 1 Lubiniecki, A.S., ed. (1990) Large-Scale Mammalian Cell Culture Technology, Dekker
2. 2 Tokashiki, M. and Yokoyama, S. (1997) Bioreactors designed for animal cells. In Mammalian Cell Biotechnology in Protein Production (Hauser, H. and Wagner, R., eds), pp. 279-317, de Gruyter, Berlin, Germany
3. 3 Aunins, J.G. and Henzler, H-J. (1993) Aeration in cell culture bioreactors. In Biotechnology (Vol. 3, 2nd edn) (Rehm, H-J. and Reed, G., eds), pp. 219-281, VCH
4. 4 Chisti, Y. (1999) Mass transfer. In Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (Vol. 3) (Flickinger, M.C. and Drew, S.W., eds), pp. 1607-1640, John Wiley
5. 5 Chalmers, J.J. (1998) Gas bubbles and their influence on microorganisms. Appl. Mech. Rev. 51, 113-120
6. 6 Arathoon, W.R. and Birch, J.R. (1986) Large-scale cell culture in biotechnology. Science 232, 1390-1395
7. 7 Birch, J.R. et al. (1987) Antibody production with airlift fermentors. In Large Scale Cell Culture Technology (Lydersen, B.K., ed.), pp. 1-20, Hanser Publishers, New York, USA
8. 8 Chisti, Y. (1993) Animal cell culture in stirred bioreactors: observations on scale-up. Bioprocess Eng. 9, 191-196
9. 9 Handa, A. et al. (1987) On the evaluation of gas-liquid interfacial effects on hybridoma viability in bubble column bioreactors. Dev. Biol. Stand. 66, 241-253
10. 10 Kioukia, N. et al. (1996) Influence of agitation and sparging on the growth rate and infection of insect cells in bioreactors and a comparison with hybridoma culture. Biotechnol. Prog. 12, 779-785
11. 11 Tramper, J. et al. (1987) Bubble column design for growth of fragile insect cells. Bioprocess Eng. 2, 37-41
12. 12 Oh, S.K.W. et al. (1992) Further studies of the culture of mouse hybridomas in an agitated bioreactor with and without continuous sparging. J. Biotechnol. 22, 245-270
13. 13 Handa-Corrigan, A. et al. (1989) Effect of gas-liquid interfaces on the growth of suspended mammalian cells: mechanisms of cell damage by bubbles. Enzyme Microb. Technol. 11, 230-235
14. 14 Cruz, P.E. et al. (1998) Optimization of the production of virus-like particles in insect cells. Biotechnol. Bioeng. 60, 408-418
15. 15 Martens, D.E. et al. (1992) Effect of serum concentration on hybridoma viable cell density and production of monoclonal antibodies in CSTRs and on shear sensitivity in air-lift loop reactors. Biotechnol. Bioeng. 39, 891-897
16. 16 Michaels, J.D. et al. (1996) Sparging and agitation-induced injury of cultured animal cells: do cell-to-bubble interactions in the bulk liquid injure cells? Biotechnol. Bioeng. 51, 399-409
17. 17 Chisti, Y. (1999) Shear sensitivity. In Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (Vol. 5) (Flickinger, M.C. and Drew, S.W., eds), pp. 2379-2406, John Wiley
18. 18 Tramper, J. et al. (1987) Bioreactor design for growth of shear-sensitive insect cells. In Plant and Animal Cells: Process Possibilities (Webb, C. and Mavituna, F., eds), pp. 125-136, Ellis Horwood, Chichester, UK
19. 19 Emery, A.N. et al. (1987) Large-scale hybridoma culture. In Plant and Animal Cells: Process Possibilities (Webb, C. and Mavituna, F., eds), pp. 137-146, Ellis Horwood, Chichester, UK
20. 20 van der Pol, L. et al. (1992) Effect of low serum concentrations (0%-2.5%) on growth, production, and shear sensitivity of hybridoma cells. Biotechnol. Bioeng. 40, 179-182
21. 21 Jöbses, I. et al. (1991) Lethal events during gas sparging in animal cell culture. Biotechnol. Bioeng. 37, 484-490
22. 22 Meier, S.J. et al. (1999) Cell death from bursting bubbles: role of cell attachment to rising bubbles in sparged reactors. Biotechnol. Bioeng. 62, 468-478
23. 23 Chisti, Y. (1989) Airlift Bioreactors, Elsevier
24. 24 Chisti, Y. (1998) Pneumatically agitated bioreactors in industrial and environmental bioprocessing: hydrodynamics, hydraulics and transport phenomena. Appl. Mech. Rev. 51, 33-112
25. 25 Grima, E. et al. (1997) Characterization of shear rates in airlift bioreactors for animal cell culture. J. Biotechnol. 54, 195-210
26. 26 Wu, J. and Goosen, F.A. (1995) Evaluation of the killing volume of gas bubbles in sparged animal cell culture bioreactors. Enzyme Microb. Technol. 17, 1036-1042
27. 27 Al-Rubeai, M. et al. (1995) Cell-cycle and cell-size dependence of susceptibility to hydrodynamic-forces. Biotechnol. Bioeng. 46, 88-92
28. 28 Lakhotia, S. et al. (1992) Damaging agitation intensities increase DNA-synthesis rate and alter cell-cycle phase distributions of CHO cells. Biotechnol. Bioeng. 40, 978-990
29. 29 Wang, N.S. et al. (1994) Unified modelling framework of cell death due to bubbles in agitated and sparged bioreactors. J. Biotechnol. 33, 107-122
30. 30 Abu-Reesh, I. and Kargi, F. (1989) Biological responses of hybridoma cells to defined hydrodynamic shear stress. J. Biotechnol. 9, 167-178
31. 31 Abu-Reesh, I. and Kargi, F. (1991) Biological responses of hybridoma cells to hydrodynamic shear in an agitated bioreactor. Enzyme Microb. Technol. 13, 913-919
32. 32 Chisti, Y. and Moo-Young, M. (1986) Disruption of microbial cells for intracellular products. Enzyme Microb. Technol. 8, 194-204
33. 33 Garrido, F. et al. (1994) Disruption of a recombinant yeast for the release of β-galactosidase. Bioseparation 4, 319-328
34. 34 McQueen, A. et al. (1987) Flow effects on the viability and lysis of suspended mammalian cells. Biotechnol. Lett. 9, 831-836
35. 35 Tamer, I.M. et al. (1998) Disruption of Alcaligenes latus for recovery of poly(β-hydroxybutyric acid): comparison of high-pressure homogenization, bead milling, and chemically induced lysis. Ind. Eng. Chem. Res. 37, 1807-1814
36. 36 van der Pol, L.A. et al. (1995) Polyethylene glycol as protectant against damage caused by sparging for hybridoma suspension cells in a bubble columns. Enzyme Microb. Technol. 17, 401-407
37. 37 Zhang, Z. et al. (1995) Effects of the hydrodynamic environment and shear protectants on survival of erythrocytes in suspension. J. Biotechnol. 43, 33-40
38. 38 Boulton-Stone, J.M. and Blake, J.R. (1993) Gas bubbles bursting at a free surface. J. Fluid Mech. 254, 466-473
39. 39 Garcia-Briones, M.A. et al. (1994) Computer simulations of the rupture of a gas bubble at a gas-liquid interface and its implications in animal cell damage. Chem. Eng. Sci. 49, 2301-2320
40. 40 Chalmers, J.J. and Bavarian, F. (1991) Microscopic visualization of insect cell-bubble interactions, II: the bubble film and bubble rupture. Biotechnol. Prog. 7, 151-158
41. 41 Cherry, R.S. and Hulle, C.T. (1992) Cell death in thin films of bursting bubbles. Biotechnol. Prog. 8, 11-18
42. 42 Garcia-Briones, M.A. and Chalmers, J.J. (1994) Flow parameters associated with hydrodynamic cell injury. Biotechnol. Bioeng. 44, 1089-1098
43. 43 Bavarian, F. et al. (1991) Microscopic visualization of insect cell-bubble interactions, I: rising bubbles, air-medium interface, and the foam layer. Biotechnol. Prog. 7, 140-150
44. 44 Trinh, K. et al. (1994) Quantification of damage to suspended insect cells as a result of bubble rupture. Biotechnol. Bioeng. 43, 37-45
45. 45 Augenstein, D.C. et al. (1971) Effect of shear on the death of two strains of mammalian tissue cells. Biotechnol. Bioeng. 13, 409-418
46. 46 Chisti, Y. and Moo-Young, M. (1993) Airlift bioreactors with packed beds of immobilized biocatalysts: theoretical evaluation of the liquid circulation performance. Trans. Inst. Chem. Eng. 71C, 209-214
47. 47 Chisti, Y. and Moo-Young, M. (1994) Anchorage-dependent animal cell culture in packed beds with airlift driven liquid circulation: a theoretical analysis of oxygen transfer and comparison with stirred tank microcarrier culture system. Trans. Inst. Chem. Eng. 72C, 92-94
48. 48 Jan, D.C-H. et al. (1993) Use of a spin-filter can reduce disruption of hybridoma cells in a bioreactor. Biotechnol. Tech. 7, 351-356
49. 49 Kurata, H. and Furusaki, S. (1993) Immobilized Coffea arabica cell culture using a bubble-column reactor with controlled light intensity. Biotechnol. Bioeng. 42, 494-502
50. 50 Sucker, H.G. et al. (1994) Bubble bed reactor: a reactor design to minimize the damage of bubble aeration on animal cells. Biotechnol. Bioeng. 44, 1246-1254
51. 51 Papoutsakis, E.T. (1991) Media additives for protecting freely suspended animal cells against agitation and aeration damage. Trends Biotechnol. 9, 316-324
52. 52 Wu, J. (1995) Mechanism of animal cell damage associated with gas bubbles and cell protection by medium additives. J. Biotechnol. 43, 81-94
53. 53 Croughan, M.S. et al. (1989) Viscous reduction of turbulent damage in animal cell culture. Biotechnol. Bioeng. 33, 862-872
54. 54 Michaels, J.D. et al. (1991) Protection mechanisms of freely suspended animal cells (CRL 8018) from fluid-mechanical injury: viscometric and bioreactor studies using serum, Pluronic F68 and polyethylene glycol. Biotechnol. Bioeng. 38, 169-180
55. 55 Ramirez, O.T. and Mutharasan, R. (1990) The role of the plasma membrane fluidity on the shear sensitivity of hybridomas grown under hydrodynamic stress. Biotechnol. Bioeng. 36, 911-920
56. 56 Ramirez, O.T. and Mutharasan, R. (1992) Effect of serum on the plasma membrane fluidity of hybridomas: an insight into its shear protective mechanism. Biotechnol. Prog. 8, 40-50
57. 57 Lavery, M. and Nienow, A.W. (1987) Oxygen transfer in animal cell culture medium. Biotechnol. Bioeng. 30, 368-373
58. 58 Cherry, R.S. and Papoutsakis, E.T. (1988) Physical mechanisms of cell damage in microcarrier cell culture bioreactors. Biotechnol. Bioeng. 32, 1001-1014
59. 59 McQueen, A. and Bailey, J.E. (1989) Influence of serum level, cell line, flow type and viscosity on flow-induced lysis of suspended mammalian cells. Biotechnol. Lett. 11, 531-536
60. 60 Goldblum, S. et al. (1990) Protective effect of methyl cellulose and other polymers on insect cells subjected to laminar shear stress. Biotechnol. Prog. 6, 383-390
61. 61 Kunas, K.T. and Papoutsakis, E.T. (1990) The protective effect of serum against hydrodynamic damage of hybridoma cells in agitated and surface-aerated bioreactors. J. Biotechnol. 15, 57-70
62. 62 Elias, C.B. et al. (1995) Turbulent shear stress - effect on mammalian cell culture and measurement using laser doppler anemometer. Chem. Eng. Sci. 50, 2431-2440
63. 63 Hülscher, M. et al. (1990) Influence of protein concentration on mechanical cell damage and fluid dynamics in airlift reactors for mammalian cell culture. Food Biotechnol. 4, 157-166
64. 64 Chisti, Y. (1998) Strategies in downstream processing. In Bioseparation and Bioprocessing: A Handbook (Vol. 2) (Subramanian, G., ed.), pp. 3-30, Wiley-VCH
65. 65 Chisti, Y. (1998) Biosafety. In Bioseparation and Bioprocessing: A Handbook (Vol. 2) (Subramanian, G., ed.), pp. 379-415, Wiley-VCH
66. 66 van der Pol, L. and Tramper, J. (1998) Shear sensitivity of animal cells from a culture-medium perspective. Trends Biotechnol. 16, 323-328
67. 67 Murhammer, D.W. (1999) Pluronic polyols, cell protection. In Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (Vol. 4) (Flickinger, M.C. and Drew, S.W., eds), pp. 2019-2024, John Wiley
68. 68 Murhammer, D.W. and Goochee, C.F. (1990) Structural features of nonionic polyglycol polymer molecules responsible for the protective effect in sparged animal cell bioreactors. Biotechnol. Prog. 6, 142-148
69. 69 Chisti, Y. and Moo-Young, M. (1993) Aeration and mixing in vortex fermenters. J. Chem. Technol. Biotechnol. 58, 331-336
70. 70 Al-Rubeai, M. et al. (1993) A flow cytometric study of hydrodynamic damage to mammalian cells. J. Biotechnol. 31, 161-177
71. 71 Murhammer, D.W. and Goochee, C.F. (1988) Scaleup of insect cell cultures: protective effect of Pluronic F-68. Biotechnology 6, 1411-1418
72. 72 Zhang, Z. et al. (1992) The effect of Pluronic F68 on the mechanical properties of mammalian cells. Enzyme Microb. Technol. 14, 980-983
73. 73 Laouar, L. et al. (1996) Yeast response to nonionic surfactants. Enzyme Microb. Technol. 18, 433-438
74. 74 Chattopadhyay, D. et al. (1995) The protective effect of specific medium additives with respect to bubble rupture. Biotechnol. Bioeng. 45, 473-480
75. 75 Chattopadhyay, D. et al. (1995) Thermodynamic approach to explain cell adhesion to air-medium interfaces. Biotechnol. Bioeng. 48, 649-658
76. 76 Wu, J. et al. (1997) Effect of surface-active medium additives on insect cell surface hydrophobicity relating to cell protection against bubble damage. Enzyme Microb. Technol. 21, 341-348
77. 77 Michaels, J.D. et al. (1995) Analysis of cell-to-bubble attachment in sparged bioreactors in the presence of cell-protecting additives. Biotechnol. Bioeng. 47, 407-419
78. 78 Michaels, J.D. et al. (1995) Interfacial properties of cell culture media with cell-protecting additives. Biotechnol. Bioeng. 47, 420-430
79. 79 Michaels, J.D. et al. (1992) Fluid-mechanical damage of freely suspended animal cells in agitated bioreactors: effects of dextran, derivatized celluloses and polyvinyl alcohol. Chem. Eng. Commun. 118, 341-360
80. 80 Dey, D. and Emery, A.N. (1999) Problems in predicting cell damage from bubble bursting. Biotechnol. Bioeng. 65, 240-245
81. 81 Chisti, Y. and Moo-Young, M. (1999) Fermentation technology, bioprocessing, scale-up and manufacture. In Biotechnology: The Science and the Business (2nd edn) (Moses, V. et al., eds), pp. 177-222, Harwood Academic Publishers
82. 82 Thomas, C.R. (1990) Problems of shear in biotechnology. In Chemical Engineering Problems in Biotechnology (Winkler, M.R., ed.), pp. 23-93, Elsevier
83. 83 Thomas, C.R. (1993) Shear effects on cells in bioreactors. In Processing of Solid-Liquid Suspensions (Shamlou, P.A., ed.), pp. 158-191, Butterworth-Heinemann
84. 84 Papoutsakis, E.T. (1991) Fluid-mechanical damage of animal cells in bioreactors. Trends Biotechnol. 9, 427-437
85. 85 Merchuk, J.C. (1991) Shear effects on suspended cells. Adv. Biochem. Eng. Biotechnol. 44, 65-95
86. 86 Prokop, A. and Bajpai, R.K. (1992) The sensitivity of biocatalysts to hydrodynamic shear stress. Adv. Appl. Microbiol. 37, 165-232
87. 87 Cherry, R.S. (1993) Animal cells in turbulent fluids: details of the physical stimulus and the biological response. Biotechnol. Adv. 11, 279-299
88. 88 Hua, J. et al. (1993) Review of the effects of shear and interfacial phenomena on cell viability. Crit. Rev. Biotechnol. 13, 305-328
89. 89 Joshi, J.B. et al. (1996) Role of hydrodynamic shear in the cultivation of animal, plant and microbial cells. Chem. Eng. J. 62, 121-141