Fed-batch CHO cell t-PA production and feed glutamine replacement to reduce ammonia production

Biotechnology Progress

Volumn 29, Issue 1, 2013, Pages 165-175

  Kim, Do Yun ^a   Chaudhry, Muhammad Arshad ^a   Kennard, Malcolm L ^a   Jardon, Mario A ^a,b   Braasch, Katrin ^c   Dionne, Ben ^c   Butler, Michael ^c   Piret, James M ^a,b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Ammonia; Chinese hamster ovary cells; Fed batch; Glutamine; Plant hydrolysate; Pyruvate; Tissue plasminogen activator

Indexed keywords

CHINESE HAMSTER OVARY CELLS; FED BATCHES; GLUTAMINE; PLANT HYDROLYSATE; PYRUVATES; TISSUE PLASMINOGEN ACTIVATOR;

AMMONIA; CELL CULTURE; CELLS; OPTIMIZATION; RECOMBINANT PROTEINS; TISSUE;

AMINO ACIDS;

AMMONIA; GLUTAMINE; TISSUE PLASMINOGEN ACTIVATOR;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL SURVIVAL; CHEMISTRY; CHO CELL; CRICETULUS; CULTURE MEDIUM; CULTURE TECHNIQUE; HAMSTER; METABOLISM; OXIDATION REDUCTION REACTION;

AMMONIA; ANIMALS; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; GLUTAMINE; OXIDATION-REDUCTION; TISSUE PLASMINOGEN ACTIVATOR;

CRICETULUS GRISEUS; GOSSYPIUM HIRSUTUM; TRITICUM AESTIVUM;

EID: 84873323391     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1658     Document Type: Article

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