Increased t-PA yields using ultrafiltration of an inhibitory product from CHO fed-batch culture

Biotechnology Progress

Volumn 16, Issue 5, 2000, Pages 786-794

  Dowd, J E ^a   Kwok, K E ^a   Piret, J M ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CHINESE HAMSTER OVARY CELLS; FED BATCH CONCENTRATE; HYBRIDOMA CULTURE; HYDROLYSATES; VIABILITY INDEX;

CELL SURVIVAL; CHO CELL; FED BATCH CULTURE; GLUCOSE; HYBRIDOMA CELL CULTURE; MESSENGER RNA; TISSUE PLASMINOGEN ACTIVATOR;

AMINO ACIDS; CELLS; CHEMICAL REACTORS; GLUCOSE; MONOCLONAL ANTIBODIES; ULTRAFILTRATION;

BATCH CELL CULTURE;

ANIMALS; CHO CELLS; CRICETINAE; CULTURE MEDIA; GLUCOSE; HYBRIDOMAS; OSMOLAR CONCENTRATION; PROTEIN BINDING; TISSUE PLASMINOGEN ACTIVATOR;

CRICETINAE; CRICETULUS GRISEUS;

EID: 0034281805     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000079b     Document Type: Article

References (44)

1. Reciprocal regulation of glucose and glutamine utilization by cultured human diploid fibroblasts
2. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells
3. Fed batch cultivation of animal cells using different medium design concepts and feeding strategies
4. The effect of ammonium on the O-linked glycosylation of granulocyte colony-stimulating factor produced by Chinese Hamster Ovary cells
5. High-density fed-batch cultures for hybridoma cells performed with the aid of a kinetic model
6. Monitoring of the production of monoclonal antibodies by hybridomas. Part 1: Long-term cultivation in hollow-fibre bioreactors using serum-free medium
7. Comparison of cell propagation methods for their effect on monoclonal antibody yield in fermentors
8. Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth
9. Characterization of a recombinant antibody produced in the course of a high yield fed-batch process
10. High viable cell concentration fed batch cultures of hybridoma cells through on line nutrient feeding
11. Growth characteristics in fed-batch culture of hybridoma cells with control of glucose and glutamine concentrations
12. Fed-batch culture of hybridoma cells: Comparison of optimal control approach and closed loop strategies
13. On line characterization of a hybridoma cell culture process
14. Effective t-PA production by BHK cells in nutrients-controlled culture using an on-line HPLC measuring system
15. Comparison of control techniques for baker's yeast culture using an automatic glucose analyzer
16. Feeding strategies for enhanced hybridoma productivity: Automated concentrate supplementation
17. Improving an on-line feeding strategy for fed-batch cultures of hybridoma cells by dialysis and 'nutrient-split'-feeding
18. Adaptive control at low glucose concentration of HEK-293 cell serum-free cultures
19. The enhancement of specific antibody production rate in glucose- and glutamine-controlled fed-batch culture
20. Catabolic control of hybridoma cells by glucose and glutamine limited fed batch cultures
21. Stoichiometric analysis of animal cell growth and its application in medium design
22. Production of recombinant factor VIII from perfusion cultures: 1. Large-scale fermentation
23. Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells
24. T-PA production through mass culturing of Chinese hamster ovary cells
25. Production of t-PA from animal cell cultures
26. Production of plasminogen activator by cells in culture
27. In pursuit of the optimal fed-batch process for monoclonal antibody production
28. Limitations to the amplification and stability of human tissue-type plasminogen activator expression by Chinese Hamster Ovary cells
29. Precolumn phenylisothiocyanate derivatization and liquid chromatography of free amino acids in biological samples
30. Amino acid analysis using phenylisothiocyanate derivatives
31. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction
32. Monoclonal antibody process development using medium concentrates
33. Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production
34. Investigation of NS0 cell metabolic behaviour in MAb producing clones
35. Effects of abrupt and gradual osmotic stress on antibody production and content in hybridoma cells that differ in production kinetics
36. Apoptosis and nutrition: Involvement of amino acid transport system in repression of hybridoma cell death
37. Predictive control of hollow-fiber bioreactors for the production of monoclonal antibodies
38. Evidence that monoclonal antibody production kinetics is related to the integral of viable cells in batch systems
39. Alteration of mammalian cell metabolism by dynamic nutrient feeding
40. Overview on fibrinolysis: Plasminogen activation pathways on fibrin and cell surfaces
41. The regulation of tissue plasminogen activator activity by human fibroblasts
42. Endothelial cell surface actin serves as a binding site for plasminogen, tissue plasminogen activator and lipoprotein(a)
43. Acoustic cell filter for high-density perfusion culture of hybridoma cells