Proteomic understanding of intracellular responses of recombinant Chinese hamster ovary cells cultivated in serum-free medium supplemented with hydrolysates

Applied Microbiology and Biotechnology

Volumn 89, Issue 6, 2011, Pages 1917-1928

  Kim, Jee Yon ^a   Kim, Yeon Gu ^a   Han, Young Kue ^a   Choi, Hyun Soo ^b   Kim, Young Hwan ^b   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Basic Science Institute (KBSI)   (South Korea)

Author keywords

Cell growth; CHO cells; Hydrolysates; Productivity; Proteomics

Indexed keywords

ANTI-PROLIFERATIVE; APOPTOTIC PROTEINS; CANDIDATE GENES; CELL ENGINEERING; CHINESE HAMSTER OVARY CELLS; CHO CELL; CYTOSKELETONS; HYDROLYSATES; NANO-LC; PROTEIN PRODUCTION; PROTEOMES; PROTEOMIC ANALYSIS; PROTEOMIC APPROACHES; PROTEOMICS; SERUM-FREE MEDIUM; SPECIFIC GROWTH RATE; TWO-DIMENSIONAL GEL ELECTROPHORESIS; WESTERN-BLOT ANALYSIS;

ANTIBODIES; BODY FLUIDS; CELL GROWTH; ELECTROPHORESIS; GENES; GROWTH KINETICS; MOLECULAR BIOLOGY;

PRODUCTIVITY;

ALPHA ACTIN; ANNEXIN; ASPP2 PROTEIN; BETA ACTIN; BETA TUBULIN; CALRETICULIN; CHAPERONIN; CYTOCHROME B5; ENOLASE; GALECTIN 1; GLUCOSE REGULATED PROTEIN 78; GLUTATHIONE TRANSFERASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HEAT SHOCK PROTEIN 60; HEAT SHOCK PROTEIN 65; HEAT SHOCK PROTEIN 70; IMMUNOGLOBULIN LIGHT CHAIN; N MYC DOWNSTREAM REGULATED GENE 1; PEROXIREDOXIN 6; PHOSPHOGLYCERATE KINASE; PHOSPHOPROTEIN; PLAKOGLOBIN; PROTEIN DISULFIDE ISOMERASE; PYRUVATE KINASE; SOY HYDROLYSATE; TROPOMYOSIN; TUMOR PROTEIN; UNCLASSIFIED DRUG; VIMENTIN; WHEAT GLUTEN HYDROLYSATE; YEAST AUTOLYSATE MEDIUM;

ANTIBODY; APOPTOSIS; CELL ORGANELLE; ELECTROKINESIS; GEL; GENE EXPRESSION; GROWTH RATE; HYDROLYSIS; IDENTIFICATION METHOD; METABOLISM; OPTIMIZATION; PROTEIN; PROTEOMICS; RECOMBINATION; RODENT; SERUM; TWO-DIMENSIONAL MODELING;

ANIMAL CELL; ANTIBODY PRODUCTION; APOPTOSIS; ARTICLE; CELL ACTIVITY; CELL GROWTH; CELL PROLIFERATION; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; CYTOMETRY; DOWN REGULATION; ELECTROSPRAY MASS SPECTROMETRY; FEMALE; GEL ELECTROPHORESIS; GROWTH RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTRACELLULAR SIGNALING; LIQUID CHROMATOGRAPHY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN METABOLISM; PROTEOMICS; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS; UPREGULATION; WESTERN BLOTTING;

ANIMALS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA, SERUM-FREE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; EPITHELIAL CELLS; PROTEOME; PROTEOMICS; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS;

EID: 79952573871     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-011-3106-9     Document Type: Article

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