Reduction of ammonia accumulation and improvement of cell viability by expression of urea cycle enzymes in Chinese Hamster ovary cells

Journal of Microbiology and Biotechnology

Volumn 13, Issue 2, 2003, Pages 217-224

  Chung, Myung Il ^d   Lim, Mi Hee ^d   Lee, Yun Jeong ^d   Kim, Ik Hwan ^a   Kim, Ick Young ^a   Kim, Jung Hoe ^b   Chang, Kern Hee ^c   Kim, Hong Jin ^d  

^a Korea University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Samsung Fine Chemicals Co   (South Korea)

^d Chung Ang University   (South Korea)

Author keywords

Ammonia concentration; Transfection cell viability

Indexed keywords

AMMONIA;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL GROWTH; CELL LEVEL; CELL LINE; CELL VIABILITY; CHO CELL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; FEMALE; GENE EXPRESSION; GENETIC TRANSFECTION; GROWTH RATE; NONHUMAN; UREA CYCLE;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0038702248     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. Andersen, D. C. and C. F. Goochee. 1995. The effect of ammonia on the O-linked glycosylation of granulocyte colony-stimulating factor produced by Chinese hamster ovary cell. Biotechnol Bioeng. 47: 96-105.
2. Atanassov, C. L., C. D. Muller, S. Sarhan, B. Knodgen, G. Rebel, and N. Seiler. 1994. Effect of ammonia on endocytosis, cytokine production and lysosomal enzyme activity of a microglial cell line. Res. Immunol. 145: 277-288.
3. Borys, M. C., D. I. H. Linzer, and E. T. Papoutsakis. 1994. Ammonium affects the glycosylation patterns of recombinant mouse placement lactogen-I by Chinese hamster ovary cells in a pH-dependent manner. Biotechnol. Bioeng. 43: 505-514.
4. Butler, M., T. Imamura, J. Thomas, and W. G. Thilly. 1983. High yields from microcarrier cultures by medium perfusion. J. Cell Sci. 61: 351-363.
5. Butler, M. and R. E. Spier. 1984. The effects of glutamine utilization and ammonia production on the growth of BHK cells in microcarrier culture. J. Biotechnol. 1: 187-196.
6. Capiaumont, J., C. Legrand, D. Carbonell, B. Dousset, F. Belleville, and P. Nabet. 1995. Methods for reducing the ammonia in hybridoma cell cultures. J. Biotechnol. 39: 49-58.
7. Carbonell, D., B. Besnainou, J. Capiaumont, C. Legrand, P. Lessart, and P. Nabet. 1995. Development of strategies for the removal of ammonia from animal cell cultures. In: Proceedings of the Eleventh ESACT Meeting, Brighton, UK, pp. 166-169.
8. Chung, B. S., Y. T. Jeong, K. H. Chang, J. S. Kim, and J. H. Kim. 2001. Effect of sodium butyrate on glycosylation of recombinant erythropoietin. J. Microbiol. Biotechnol. 11: 1087-1092.
9. Cruz, H. J., C. M. Freitas, P. M. Alves, J. L. Moreira, and M. J. T. Carrondo. 2000. Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells. Enz. Microbial Technol. 27: 43-52.
10. Darling, T., J. Mitschelen, C. Kaufman, S. Dilworth, E. Sanders, T. Irish, M. Dombalagian, and T. O. Sun. 1988. The control of ammonia toxicity in large scale culture of C 127 cells and hybridomas. Abstract Paper, Chemical Congress of North Am. 3(2).
11. Dingemase, M. A., W. J. De Jonge, P. A. De Boer, M. Mori, and W. H. Lamers. 1996. Development of the ornithine cycle in rat liver: Zonation of a metabolic pathway. Hepatology 24: 407-411.
12. Frederick, M. A., B. Roger, E. K. Robert, D. M. David, J. G. Seidmon, A. S. John, and S. Kevin. 1997. Current Protocols in Molecular Biology. Wiley, NY, U.S.A.
13. Glacken, M. W., R. J. Fleischaker, and A. J. Sinskey. 1986. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells. Biotechnol. Bioeng. 28: 1376-1389.
14. Hansen, H. A. and C. Emborg. 1994. Influence of ammonium on growth, metabolism, and productivity of a continuous suspension Chinese hamster ovary cell culture. Biotechnol. Prog. 10: 121-124.
15. Hassell, T. and M. Butler. 1990. Adaptation to non-ammoniagenic medium and selective substrate feeding lead to enhanced yields in animal cell culture. J. Cell Sci. 96: 501-508.
16. Hiller, G. W., D. S. Clark, and H. W. Blanch. 1994. Transient responses of hybridoma cells in continuous culture to step changes in amino acids and vitamin concentrations. Biotechnol. Bioeng. 44: 303-321.
17. Iio, M., A. Moriyama, and H. Murakami. 1984. Effects on cell proliferation of metabolites produced by cultured cells and their removal from culture in defined media, pp. 437-442. In Murakami et al. (eds.), Proceedings of the International Symposium on Growth and Differentiation of Cells in Defined Environment. Fukuoka, Japan.
18. Jeong, Y. H., H. I. Lee, G. T. Chun, I. H. Kim, and S. S. Wang. 1996. Ammonium ion effects and its in situ removal by using immobilized adsorbent in hybridoma cell culture. Kor. J. Biotechnol. Bioeng. 11: 329-339.
19. Kawamoto, S., Y. Amaya, K. Murakami, and F. Tokunaga. 1987. Complete nucleotide sequence of cDNA and deduced amino acid sequence of rat liver arginase. J. Biol. Chem. 262: 6280-6283.
20. Klucinski, W. and S. Tarowski. 1984. Ammonia toxicity for mammalian and avian lymphocytes from blood. Immunopharmacology 8: 47-52.
21. Kurano, N., C. Leist, F. Messi, S. Kurano, and A. Fiechter. 1990. Growth behavior of Chinese hamster ovary cells in a compact loop bioreactor, 2. Effects of medium components and waster products. J. Biotechnol. 15: 113-129.
22. Lambert, M. A., L. R. Simard, P. N. Ray, and R. R. Mcinnes. 1986. Molecular cloning of cDNA for rat argininosuccinate lyase and its expression in rat hepatoma cell lines. Mol. Cell. Biol. 6: 1722-1728.
23. Ljunggren, J. and L. Haggstrom. 1994. Catabolic control of hybridoma cells by glucose and glutamine limited fed batch cultures. Biotechnol. Bioeng. 44: 808-818.
24. Mc Queen, A. and J. Bailey. 1990. Effect of ammonium ion and extracellular pH on hybridoma cell metabolism and antibody production. Biotechnol. Bioeng. 35: 1067-1077.
25. Moorman, A. F., P. A. De Boer, A. T. Das, W. T. Labruyere, R. Charles, and W. H. Lamers. 1990. Expression patterns of mRNAs for ammonia-metablizing enzymes in the developing rat: The ontogenesis of hepatocyte heterogeneity. Histochem. J. 22: 457-468.
26. Nayve, F. R. P. Jr, T. Misato, M. Matsumura, and H. Kataoka. 1994. HBs-Mab production in perfusion culture with selective ammonia removal system. J. Biotechnol. 34: 217-225.
27. Ozturk, S. S., M. R. Riley, and B. O. Palsson. 1992. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production. Biotechnol. Bioeng. 39: 418-431.
28. Park, H. S., I. H. Kim, I. Y. Kim, K. H. Kim, and H. J. Kim. 2000. Expression of carbamoyl phosphate synthetase I and ornithine transcarbamoylase genes in Chinese hamster ovary dhfr-cells decreases accumulation of ammonium ion in culture media. J. Biotechnol. 8: 1129-1140.
29. Ryu, H. W., H. D. Han, and K. S. Cho. 2002. Characteristics of ammonia removal in biofilters inoculated with earthworm cast. Kor. J. Microbiol. Biotechnol. 30: 73-78.
30. Sambrook, J., E. F. Fritisch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, Second ed. Cold Spring Harbor, Laboratory Press, Cold Spring Harbor, NY, U.S.A.
31. Sikdar, S. K. and S. B. Sawant. 1994. Ammonia removal from mammalian cell culture medium by ion-exchange membranes. Sep. Sci. Technol. 29: 1579-1591.
32. Singh, R. P., M. Al Rubeai, C. D. Gregory, and A. N. Emery. 1994. Cell death in bioreactors: A role for apoptosis. Biotechnol. Bioeng. 44: 720-726.
33. Thommes, J., U. Garske, M. Biselli, and C. Wandrey. 1992. Integrated detoxification: Reduction of ammonium concentration by dialysis with cation exchange membranes. In: Proceedings of the Eleventh ESACT Meeting, Brighton, UK, pp. 171-175.
34. Thorens, B. and P. Vassalli. 1986. Chloroquine and ammonium chloride prevents terminal glycosylation of immunoglobulins in plasma cells without affecting secretion. Nature 321: 618-620.
35. Visek, W., G. Kolodny, and P. Gross. 1972. Ammonia effects in culture of normal and transformed 3T3 cells. J. Cell. Physiol. 80: 372-382.
36. Wagenaar, G. T., W. J. Geerts, R. A. Chamuleau, N. E. Dertz, and W. H. Lamers. 1994. Lobular patterns of expression and enzyme activities of glutamine synthase, carbamoylphosphate synthase and glutamate dehydrogenase during postnatal development of the porcine liver. Biochim. Biophys. Acta 1200: 265-270.
37. Westlund, A. and L. Haggstrom. 1997. Ammonium ion transport by the Na+K+2Cl cotransporter induces apoptosis in hybridoma cells. Biotechnol. Lett. 20: 87-90.
38. Yang, M. and M. Butler. 2000. Effects of ammonia on CHO cell growth, erythropoietin production, and glycosylation. Biotechnol. Bioeng. 68: 370-380.
39. Zanghi, J. A., T. P. Mendoza, A. E. Schmelzer, R. H. Knop, and W. M. Miller. 1998. Role of nucleotide sugar pools in the inhibition of NCAM polysialylation by ammonia. Biotechnol. Prog. 14: 834-844.