An HPLC-MALDI MS method for N-glycan analyses using smaller size samples: Application to monitor glycan modulation by medium conditions

Glycoconjugate Journal

Volumn 26, Issue 9, 2009, Pages 1135-1149

  Gillmeister, Michael P ^a   Tomiya, Noboru ^b   Jacobia, Scott J ^c   Lee, Yuan C ^b   Gorfien, Stephen F ^c   Betenbaugh, Michael J ^a  

^a Johns Hopkins University   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

^c LIFE TECHNOLOGIES INC   (United States)

Author keywords

Antibody; Cell culture; HPLC; Mass spectrometry; Media; N glycan analysis; PH

Indexed keywords

ALANINE; ALANYLGLUTAMINE; ALTEPLASE; AMMONIA; GLUTAMINE; GLYCAN; IMMUNOGLOBULIN G ANTIBODY; OLIGOSACCHARIDE; ACID; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; GLYCOPROTEIN; IMMUNOGLOBULIN G; OCTADECYLSILANE; POLYSACCHARIDE; SILANE DERIVATIVE;

ANIMAL CELL; ARTICLE; CHINESE HAMSTER; CONTROLLED STUDY; GLYCOSYLATION; ISOMER; LYSOSOME; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ANIMAL; CARBOHYDRATE ANALYSIS; CHEMISTRY; CHO CELL; CRICETULUS; CULTURE MEDIUM; DRUG EFFECT; HAMSTER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; SAMPLE SIZE; STAINING;

GLUTA;

ACIDS; ANIMALS; CARBOHYDRATE SEQUENCE; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; CULTURE MEDIA; GLYCOPROTEINS; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; INDICATORS AND REAGENTS; LYSOSOMES; MOLECULAR SEQUENCE DATA; POLYSACCHARIDES; SAMPLE SIZE; SILANES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAINING AND LABELING;

EID: 77649268393     PISSN: 02820080     EISSN: 15734986     Source Type: Journal    
DOI: 10.1007/s10719-009-9235-z     Document Type: Article

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