Ammonium ion and glucosamine dependent increases of oligosaccharide complexity in recombinant glycoproteins secreted from cultivated BHK-21 cells

Biotechnology and Bioengineering

Volumn 57, Issue 5, 1998, Pages 518-528

  Gawlitzek, Martin ^a,b   Valley, Ulrich ^a,c   Wagner, Roland ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b GENENTECH INC   (United States)

^c UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Ammonium; BHK 21 cells; N glycosylation; UDP GlcNAc

Indexed keywords

AMINES; AMINO ACIDS; AMMONIUM COMPOUNDS; BIOREACTORS; COMPOSITION EFFECTS; GROWTH KINETICS; MOLECULAR STRUCTURE; OLIGOMERS; POLYSACCHARIDES; PROTEINS; PURIFICATION;

GLUCOSAMINE; GLUTAMINE; GLYCOPROTEINS;

ANIMAL CELL CULTURE;

AMMONIA; ASPARAGINE LINKED OLIGOSACCHARIDE; GLUCOSAMINE; GLUTAMINE; GLYCAN DERIVATIVE; RECOMBINANT INTERLEUKIN 2; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE;

AMPEROMETRY; ANIMAL CELL; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CELL STRAIN BHK; MASS SPECTROMETRY; METHYLATION; NONHUMAN;

ACETYLGALACTOSAMINE; ACETYLGLUCOSAMINE; ANIMALS; CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; CELLS, CULTURED; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; GLUCOSAMINE; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; INTERLEUKIN-2; KIDNEY; METHYLATION; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; QUATERNARY AMMONIUM COMPOUNDS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; URIDINE DIPHOSPHATE;

EID: 0032485239     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980305)57:5<518::AID-BIT3>3.0.CO;2-J     Document Type: Article

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