Effects of high concentrations of energy sources and metabolites on suspension culture of Chinese hamster ovary cells producing tissue plasminogen activator

Journal of Bioscience and Bioengineering

Volumn 91, Issue 5, 2001, Pages 515-521

  Takagi, Mutsumi ^a   Hia, Hui Ching ^a   Jang, Jin Ho ^a   Yoshida, Toshiomi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

CHO cells; Glucose; Glutamine; High concentration; tPA

Indexed keywords

AMINO ACIDS; AMMONIA; CELL CULTURE; GLUCOSE; TISSUE; VITAMINS;

ENERGY SOURCES;

METABOLITES;

AMINO ACID; AMMONIA; GLUCOSE; GLUTAMINE; LACTIC ACID; TISSUE PLASMINOGEN ACTIVATOR; VITAMIN;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL METABOLISM; CELL SUSPENSION; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; ENERGY RESOURCE; NONHUMAN; PROTEIN SYNTHESIS; SERUM;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0034976298     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(01)80283-8     Document Type: Article

References (6)

1. A low-serum medium with BSA and ferrous sulfate for the production of tissue plasminogen activator by human embryo lung cells
2. The effect of osmolarity on metabolism and morphology in adhesion and suspension Chinese hamster ovary cells producing tissue plasminogen activator
3. Effects of glucose on the production of recombinant protein C in mammalian cell culture
4. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production
5. Metabolic flux change in hybridoma cells under high osmotic pressure
6. Interactive dual control of glucose and glutamine feeding in hybridoma cultivation