Substitution of glutamine by glutamate enhances production and galactosylation of recombinant IgG in Chinese hamster ovary cells

Applied Microbiology and Biotechnology

Volumn 88, Issue 4, 2010, Pages 869-876

  Hong, Jong Kwang ^a   Cho, Sung Min ^a   Yoon, Sung Kwan ^a  

^a Aprogen   (South Korea)

Author keywords

CDC activity; CHO cells; Galactosylation; Glutamate; Recombinant IgG

Indexed keywords

CDC ACTIVITY; CHO CELLS; GALACTOSYLATION; GLUTAMATE; RECOMBINANT IGG;

AMINO ACIDS; AMMONIA; BOTTLES; GROWTH KINETICS;

CELL GROWTH;

AMMONIA; GLUTAMIC ACID; GLUTAMINE; RECOMBINANT IMMUNOGLOBULIN G; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; IMMUNOGLOBULIN G;

AMMONIA; CELL ORGANELLE; GROWTH RATE; RECOMBINATION; RODENT; TOXICITY;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL GROWTH; CHEMICAL MODIFICATION; CHO CELL; CYTOTOXICITY; GALACTOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PROTEIN PURIFICATION; ANIMAL; BIOSYNTHESIS; CRICETULUS; CULTURE MEDIUM; FEMALE; HAMSTER; METABOLISM;

CRICETULUS GRISEUS;

AMMONIA; ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA, SERUM-FREE; FEMALE; GLUTAMIC ACID; GLUTAMINE; IMMUNOGLOBULIN G;

EID: 79952108569     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2790-1     Document Type: Article

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