Glycosylation of a recombinant protein in the Tn5B1-4 insect cell line: Influence of ammonia, time of harvest, temperature, and dissolved oxygen

Biotechnology and Bioengineering

Volumn 63, Issue 3, 1999, Pages 255-262

  Donaldson, Michael ^a   Wood, H Alan ^b   Kulakosky, Peter C ^b   Shuler, Michael L ^a  

^a Cornell University   (United States)

^b United States Department of Agriculture Agricultural Research Service and Boyce Thompson Institute for Plant Research   (United States)

Author keywords

Ammonia; Baculovirus; Culture conditions; Dissolved oxygen; Environment; Fluorescence assisted carbohydrate electrophoresis; Glycosylation; Human secreted alkaline phosphatase; Insect; Temperature; Time of harvest

Indexed keywords

AMMONIA; ELECTROPHORESIS; ENZYMES; OXYGEN; PROTEINS; TEMPERATURE; VIRUSES;

BACULOVIRUS; GLYCOSYLATION; HUMAN SECRETED ALKALINE PHOSPHATASE; INSECT; RECOMBINANT PROTEIN;

CELL CULTURE;

ALKALINE PHOSPHATASE; AMMONIA; BETA N ACETYLHEXOSAMINIDASE; DISSOLVED OXYGEN; MANNOSIDASE; OLIGOSACCHARIDE;

ARTICLE; BACULOVIRUS; CELL CULTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; NONHUMAN; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; STRUCTURE ANALYSIS; TEMPERATURE;

ALKALINE PHOSPHATASE; AMMONIA; ANIMALS; BACULOVIRIDAE; CELL CULTURE TECHNIQUES; CELL LINE; CULTURE MEDIA; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLYCOSYLATION; HUMANS; INSECTS; OXYGEN; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; TEMPERATURE; TRANSFECTION;

INSECTA; INVERTEBRATA; UNIDENTIFIED BACULOVIRUS;

EID: 0033526408     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990505)63:3<255::AID-BIT1>3.0.CO;2-R     Document Type: Article

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