Probing protein surface with a solvent mimetic carbene coupled to detection by mass spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 1, 2012, Pages 30-42

  Gómez, Gabriela E ^a   Mundo, Mariana R ^a   Craig, Patricio O ^a,b   Delfino, José M ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Accessible surface area; Diazirine; Methylene carbene; Photochemical probe; Protein conformation

Indexed keywords

ACCESSIBLE SURFACE AREAS; AMINO ACID SEQUENCE; CARBENES; CHEMICAL NATURE; COMPLEX FORMATIONS; DIAZIRINE; ELECTROSPRAY MASS SPECTROMETRY; HIGH RESOLUTION; KEY PARAMETERS; LIGAND BINDING; MS/MS ANALYSIS; NON-NATIVE STATE; POLYPEPTIDE CHAIN; PROTEIN CONFORMATION; PROTEIN SCIENCE; PROTEIN SURFACE; SOLVENT ACCESSIBILITY; SPECTROMETRY TECHNIQUE; TARGET SITES;

ALKYLATION; AMINO ACIDS; CONFORMATIONS; LIGANDS; MASS SPECTROMETRY; ORGANIC COMPOUNDS; PEPTIDES; PROBES; PROTEIN FOLDING; SIGNAL DETECTION; SULFONATION;

SOLVENTS;

AMINO ACID; AZIRINE DERIVATIVE; CARBENE; DIAZIRINE; PEPTIDE; POLYPEPTIDE; PROTEIN; SOLVENT; UNCLASSIFIED DRUG; WATER;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL MODIFICATION; COMPLEX FORMATION; CONFORMATION; CONTROLLED STUDY; LIGAND BINDING; MASS SPECTROMETRY; MEASUREMENT; METHYLATION; MOLECULAR MIMICRY; MOLECULAR PROBE; PEPTIDE ANALYSIS; PROTEIN FOLDING; PROTEIN STRUCTURE; SENSITIVITY AND SPECIFICITY; SIGNAL PROCESSING; SOLVENT ACCESSIBLE SURFACE AREA; SURFACE PROPERTY; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CHROMATOGRAPHY, REVERSE-PHASE; DIAZOMETHANE; HUMANS; METHANE; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PHOTOLYSIS; PROTEIN FOLDING; PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 84856179597     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-011-0266-x     Document Type: Article

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