Investigation of degradation processes in IgG1 monoclonal antibodies by limited proteolysis coupled with weak cation-exchange HPLC

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 878, Issue 11-12, 2010, Pages 868-876

  Lau, Hollis ^a   Pace, Danielle ^a   Yan, Boxu ^a   McGrath, Theresa ^a   Smallwood, Scott ^a   Patel, Ketaki ^b   Park, Jihea ^b   Park, SungAe S ^b   Latypov, Ramil F ^a  

^a AMGEN INC   (United States)

^b AMGEN INC   (United States)

Author keywords

Antibody degradation; Antibody dimerization; Cation exchange chromatography; Limited proteolysis; Mass spectrometry; Monoclonal antibody

Indexed keywords

ANTIGEN-BINDING; BIOLOGICAL ACTIVITIES; CATION EXCHANGE CHROMATOGRAPHY; CATION EXCHANGES; COMPLEMENTARITY-DETERMINING REGIONS; DEGRADATION PROCESS; DEGRADATION PRODUCTS; HEAVY CHAIN; HIGH-PURITY; HPLC METHOD; ISOFORMS; LIGHT EXPOSURE; LIGHT-INDUCED DEGRADATION; LIMITED PROTEOLYSIS; MONOCLONAL ANTIBODIES (MABS); NEW SPECIES; NONCOVALENT; PROTEIN VARIANTS; SPECTROMETRIC ANALYSIS; STORAGE CONDITION; STRUCTURAL ORGANIZATION;

ALLERGIES; CHROMATOGRAPHY; COMPLEXATION; DIMERIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PHOSPHATASES; POSITIVE IONS; SURFACE PLASMON RESONANCE;

DEGRADATION;

IMMUNOGLOBULIN G1;

ANTIGEN BINDING; ARTICLE; DIMERIZATION; FRACTIONATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN INTERACTION; STRUCTURE ANALYSIS;

ANTIBODIES, MONOCLONAL; CATION EXCHANGE RESINS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; IMMUNOGLOBULIN G; LIGHT; PROTEIN ISOFORMS; PROTEIN MULTIMERIZATION; PROTEIN PROCESSING, POST-TRANSLATIONAL;

EID: 77949487806     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2010.02.003     Document Type: Article

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