Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the "Zona Alta del Río Mendoza" region in Argentina

International Journal of Food Microbiology

Volumn 151, Issue 3, 2011, Pages 319-326

  Mercado, Laura ^a   Sturm, María Elena ^a   Rojo, María Cecilia ^a,b   Ciklic, Iván ^a   Martínez, Claudio ^c   Combina, Mariana ^a,b  

^a INSTITUTO NACIONAL DE TECNOLOGÍA AGROPECUARIA INTA   (Argentina)

^b Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

^c UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

Author keywords

Argentina; Biodiversity; Molecular markers; Saccharomyces cerevisiae; Vineyard

Indexed keywords

MITOCHONDRIAL DNA; MOLECULAR MARKER;

ARGENTINA; ARTICLE; BIODIVERSITY; CELL ISOLATION; CONTROLLED STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SACCHAROMYCES CEREVISIAE; VINEYARD; YEAST;

ARGENTINA; BIODIVERSITY; DNA, FUNGAL; DNA, MITOCHONDRIAL; FERMENTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SACCHAROMYCES CEREVISIAE; VITIS; WINE;

SACCHAROMYCES CEREVISIAE; VITACEAE;

EID: 81055140074     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2011.09.026     Document Type: Article

References (43)

1. Barrajón N., Arévalo-Villena M., Úbeda J., Briones A. Competition between spontaneous and commercial yeasts in winemaking: study of possible factors involved. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology 2010, vol. 2:1035-1041. Formatex, Badajoz. Méndez-Vilas (Ed.).
2. Bisson L.F. The biotechnology of wine yeast. Food Biotechnology 2004, 18:63-96.
3. Capece A., Romaniello R., Siesto G., Pietrafesa R., Massari C., Poeta C., Romano P. Selection of indigenous Saccharomyces cerevisiae strains for Nero d'Avola wine and evaluation of selected starter implantation in pilot fermentation. International Journal of Food Microbiology 2010, 144:187-192.
4. Clavijo A., Calderón I., Paneque P. Diversity of Saccharomyces and non-Saccharomyces yeasts in three red grape varieties cultured in the Serranía de Ronda (Spain) vine-growing region. International Journal of Food Microbiology 2010, 143:241-245.
5. Combina M., Mercado L., Borgo P., Elia A., Jofre V., Ganga A., Martinez C., Catania C. Yeasts associated to Malbec grape berries from Mendoza, Argentina. Journal of Applied Microbiology 2005, 98:1055-1061.
6. Csoma H., Zakany N., Capece A., Romano P., Sipiczki M. Biological diversity of Saccharomyces yeasts of spontaneously fermenting wines in four wine regions: comparative genotypic and phenotypic analysis. International Journal of Food Microbiology 2010, 140:239-248.
7. Cubillos F., Vásquez C., Faugeron S., Ganga A., Martínez C. Self-fertilization is the main sexual reproduction mechanism in native wine yeast populations. FEMS Microbiology Ecology 2009, 67:162-170.
8. Fernández-Espinar M.T., Esteve-Zarzoso B., Querol A., Barrio E. RFLP of the ribosomal internal transcribes spacers and the 5.8S rRNA gene region of the genus Saccharomyces: a fast method for species identification and the differentiation of flor yeasts. Antonie Van Leeuwenhoek 2000, 78:87-97.
9. Fleet G.H. Wine yeasts for the future. FEMS Yeast Research 2008, 8:979-995.
10. Fungelsang K., Edwards C. Wine Microbiology, Practical Applications and Procedures 2007, Springer Science+Business Media, New York. Second ed.
11. González S.S., Barrio E., Querol A. Molecular identification and characterization of wine yeasts isolated from Tenerife (Canary Island, Spain). Journal of Applied Microbiology 2007, 102:1018-1025.
12. Goode J. Terroir: how do soils and climate shape wine?. The Science of Wine: From Wine to Glass 2005, 25-34. University California Press, Berkeley, Los Angeles.
13. Hoffman C.S., Winston F. A ten-minute DNA preparation from yeast efficiently release autonomous plasmids for transformation of E. coli. Gene 1987, 57:267-272.
14. INV (Instituto Nacional de Vitivinicultura, Argentina) Malbec, variedad emblemática de Argentina URL:, on access march 2011. http://www.inv.gov.ar/PDF/malbec.pdf.
15. Khan W., Augustyn O.P.H., van derWesthuizen T.J., Lambrechts M.G., Pretorius I.S. Geographic distribution and evaluation of Saccharomyces cerevisiae strains isolated from vineyards in the warmer, inland regions of the Western Cape in South Africa. South African Journal of Enology and Viticulture 2000, 21:17-31.
16. Legras J., Karst F. Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterization. FEMS Microbiology Letters 2003, 221:249-255.
17. Li S., Cheng C., Li Z., Chen J., Yan B., Han B., Reeves M. Yeast species associated with wine grapes in China. International Journal of Food Microbiology 2010, 138:85-90.
18. Lopandic K., Tiefenbrunner W., Gang H., Mand K., Berger S., Leitner G., Abd-Ellah G.A., Querol A., Gardner R.C., Sterflinger K., Prillinger H. Molecular profiling of yeasts isolated during spontaneous fermentations of Austrian wines. FEMS Yeast Research 2008, 8:1063-1075.
19. Lopes C., Lavalle T., Querol A., Caballero A. Combined use of killer biotype and mtDNA-RFLP patterns in a Patagonian wine Saccharomyces cerevisiae diversity study. Antonie Van Leeuwenhoek 2005, 3:1-10.
20. Martínez C., Gac S., Lavin A., Ganga M. Genomic characterization of Saccharomyces cerevisiae strains isolated from wine-producing areas of South America. Journal of Applied Microbiology 2004, 96:1161-1168.
21. Martínez C., Cosgaya P., Vásquez C., Gac S., Ganga A. High degree of polymorphism and geographic origin of wine yeast strains. Journal of Applied Microbiology 2007, 103:2185-2195.
22. Mercado L., Dalcero A., Masuelli R., Combina M. Diversity of Saccharomyces strains on grapes and winery surfaces: analysis of their contribution to fermentative flora of Malbec wine from Mendoza (Argentina) during two consecutive years. Food Microbiology 2007, 24:403-412.
23. Mercado L., Jubany S., Gaggero C., Masuelli R.W., Combina M. Molecular relationships between Saccharomyces cerevisiae strains involved in winemaking from Mendoza, Argentina. Current Microbiology 2010, 61:506-514.
24. Ness C., Lavallee F., Dubourdieu D., Aigle M., Dulau L. Identification of yeast strains using PCR. Journal of the Science of Food and Agriculture 1993, 32:89-94.
25. Pallmann C., Brown J.A., Olineka T.L., Cocolin L., Mills D., Bisson L. Use of WL medium to profile native flora fermentations. American Journal of Enology and Viticulture 2001, 52:198-203.
26. Polsinelli M., Romano P., Suzzi G., Mortimer R. Multiple strains of Saccharomyces cerevisiae on a single grape vine. Letters in Applied Microbiology 1996, 23:110-114.
27. Pramateftaki P.V., Lanaridis P., Typas M.A. Molecular identification of wine yeast at species or strain level: a case study with strains from two vine-growing areas of Greece. Journal of Applied Microbiology 2000, 89:236-248.
28. Pretorius I.S. Tailoring wine yeast for the new millenium: novel approaches to the ancient art of winemaking. Yeast 2000, 16:1-55.
29. Querol A., Barrio E., Ramon D. A comparative study of different methods of yeast strains characterization. Systematic and Applied Microbiology 1992, 15:439-446.
30. Raspor P., Milek D.M., Polanc J., Mozina S.S., Nadez N. Yeasts isolated from three varieties of grapes cultivated in different locations of the Dolenjska vine-growing region, Slovenia. International Journal of Food Microbiology 2006, 109:97-102.
31. Ribéreau-Gayon P., Dubordieu D., Donèche B., Lombaud A. Citology, taxonomy and ecology of grape and wine yeasts. Handbook of Enology. The Microbiology of Wine and Vinifications 2006, 1-52. John Wiley& Sons Ltd., Chichester. second ed. P. Ribéreau-Gayon, D. Dubordieu, B. Donèche, A. Lombaud (Eds.).
32. Sabate J., Cano J., Querol A., Guillamon J.M. Diversity of Saccharomyces strains in wine fermentations: analysis for two consecutive years. Letters in Applied Microbiology 1998, 26:452-455.
33. Schuller D., Casal M. The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis. Antonie Van Leeuwenhoek 2007, 91:137-150.
34. Schuller D., Alves H., Dequin S., Casal M. Ecological survey of Saccharomyces cerevisiae strains from vineyards in the Vinho Verde Region of Portugal. FEMS Microbiology Ecology 2005, 51:167-177.
35. Schuller D., Pereira L., Alves H., Cambon B., Dequin S., Casal M. Genetic characterization of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments. Yeast 2007, 24:625-636.
36. Torija M.J., Rozes N., Poblet M., Guillamon J.M., Mas A. Yeast population dynamics in spontaneous fermentations: comparison between two different wine-producing areas over a period of three years. Antonie Van Leeuwenhoek 2001, 79:345-352.
37. Valero E., Schuller D., Cambon B., Casal M., Dequin S. Dissemination and survival of commercial wine yeast in the vineyard: a large-scale, three years study. FEMS Yeast Research 2005, 10:959-969.
38. Valero E., Cambon B., Schuller D., Casal M., Dequin S. Biodiversity of Saccharomyces yeast strains from grape berries from wine-producing areas using starter commercial yeasts. FEMS Yeast Research 2007, 7:317-329.
39. van der Westhuizen T.J., Augustyn O.H.P., Pretorius I.S. Geographical distribution of indigenous Saccharomyces cerevisiae strains isolated from vineyards in the coastal regions of the western Cape in South Africa. South African Journal of Enology and Viticulture 2000, 21:3-9.
40. Versavaud A., Courcoux P., Roulland C., Dulau L., Hallet J.N. Genetic diversity and geographical distribution of wild Saccharomyces cerevisiae strains from the wine producing area of Charentes, France. Applied and Environmental Microbiology 1995, 61:3521-3529.
41. Vezinhet F., Hallet J., Valade M., Poulard A. Ecological survey of wine yeast strains by molecular methods of identification. American Journal of Enology and Viticulture 1992, 43:83-86.
42. Wright S. Evolution and the Genetics of Populations 1978, vol. 4. University of Chicago Press, Chicago.
43. Yeh F.C., Boyle T.J.B. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany 1997, 129:157.