Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures

Biotechnology and Bioengineering

Volumn 107, Issue 3, 2010, Pages 516-528

  Wong, Danny Chee Furng ^a   Wong, Niki Soo Ching ^a   Goh, John Soo Yang ^a   May, Lee May ^a   Yap, Miranda Gek Sim ^b  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Chinese hamster ovary (CHO); Fed batch; Gene expression; Glycosylation; Interferon; PCR; Sialylation

Indexed keywords

BATCH CULTURE; CHINESE HAMSTER OVARY; CHO CELL; DOWN-REGULATION; FED BATCHES; FED-BATCH CULTURES; GENES EXPRESSION; GLYCOFORMS; MOLAR RATIO; N-GLYCOSYLATION; PCR; PRODUCTION PROCESS; QUANTITATIVE REAL TIME PCR; QUANTITATIVE RT-PCR; RECOMBINANT GLYCOPROTEINS; RECOMBINANT HUMAN INTERFERON; RECOMBINANT PROTEIN; SIALIC ACIDS; SIALIDASES; SIALYLATION;

AMINO ACIDS; ANTIBIOTICS; BATCH CELL CULTURE; CARBOXYLIC ACIDS; CELL CULTURE; ESTERIFICATION; GENE EXPRESSION REGULATION; GLYCOPROTEINS; GLYCOSYLATION; INTERFERONS; POLYMERASE CHAIN REACTION; GENE EXPRESSION; RECOMBINANT PROTEINS;

GENE EXPRESSION; GLYCOSYLATION;

GAMMA INTERFERON; GLUTAMINE; GLYCOPROTEIN; RECOMBINANT PROTEIN; SIALIC ACID; SIALIDASE; CYTIDINE PHOSPHATE; RECOMBINANT GAMMA INTERFERON;

ANIMAL CELL; ARTICLE; CELL DEATH; CHO CELL; CONTROLLED STUDY; FED BATCH CULTURE; FEMALE; GENE EXPRESSION; GENE EXPRESSION PROFILING; NONHUMAN; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN QUALITY; REAL TIME POLYMERASE CHAIN REACTION; RECEPTOR DOWN REGULATION; RECEPTOR UPREGULATION; SIALYLATION; CYTOKINE PRODUCTION; DOWN REGULATION; ENZYME ACTIVITY; GLYCOSYLATION; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; UPREGULATION;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION PROFILING; GLYCOPROTEINS; GLYCOSYLATION; GLYCOSYLTRANSFERASES; HUMANS; INTERFERON-GAMMA; N-ACETYLNEURAMINIC ACID; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

CRICETULUS GRISEUS;

EID: 78651471034     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22828     Document Type: Article

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