Enhancing recombinant glycoprotein sialylation through CMP-sialic acid transporter over expression in Chinese hamster ovary cells

Biotechnology and Bioengineering

Volumn 93, Issue 5, 2006, Pages 1005-1016

  Wong, Niki S C ^a   Yap, Miranda G S ^a,b   Wang, Daniel I C ^a,c  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b AGENCY FOR SCIENCE   (Singapore)

^c Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

CHO cells; CMP sialic acid transporter; Glycosylation; Over expression; Protein quality; Sialylation

Indexed keywords

BIOLOGICAL ORGANS; CELL CULTURE; CELLS; CYTOLOGY; ORGANIC ACIDS;

CHO CELLS; CMP-SIALIC ACID TRANSPORTER; GLYCOSYLATION; OVER EXPRESSION; PROTEIN QUALITY; SIALYLATION;

PROTEINS;

CYTIDINE PHOSPHATE; GAMMA INTERFERON; GLYCOPROTEIN; NUCLEOTIDE; RECOMBINANT PROTEIN; SIALIC ACID;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; GLYCOSYLATION; GOLGI COMPLEX; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN QUALITY; REAL TIME POLYMERASE CHAIN REACTION; SIALYLATION; WESTERN BLOTTING;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; CYTIDINE MONOPHOSPHATE N-ACETYLNEURAMINIC ACID; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC VECTORS; GLYCOSYLATION; HUMANS; INTERFERON-GAMMA, RECOMBINANT; MEMBRANE GLYCOPROTEINS; N-ACETYLNEURAMINIC ACID; NUCLEOTIDE TRANSPORT PROTEINS; PEANUT AGGLUTININ; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; TRANSFECTION; WHEAT GERM AGGLUTININS;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 33646029110     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20815     Document Type: Article

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