Constraints on the transport and glycosylation of recombinant IFN-γ in Chinese hamster ovary and insect cells

Biotechnology and Bioengineering

Volumn 63, Issue 5, 1999, Pages 559-572

  Hooker, Andrew D ^a,c   Green, Nicola H ^a   Baines, Anthony J ^a   Bull, Alan T ^a   Jenkins, Nigel ^a,d   Strange, Philip G ^a,b   James, David C ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

^b UNIVERSITY OF READING   (United Kingdom)

^c Oxford GlycoSciences   (United Kingdom)

^d ELI LILLY AND COMPANY   (United States)

Author keywords

CHO cells; Insect cells; Interferon; Mass spectrometry; N glycosylation; Nucleotide sugars; Secretion

Indexed keywords

CELLS; DESORPTION; LASER DIAGNOSTICS; MASS SPECTROMETRY; PROTEINS;

GALACTOSYLATION; GLYCOSYLATION; RECOMBINANT GLYCOPROTEINS;

BIOTECHNOLOGY;

CARBOHYDRATE DERIVATIVE; ISOPROTEIN; MANNOSE; RECOMBINANT GAMMA INTERFERON; SIALOGLYCOPROTEIN; SIALYLTRANSFERASE; URIDINE DIPHOSPHATE SUGAR;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL LEVEL; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; FUCOSYLATION; GOLGI COMPLEX; NONHUMAN; PROTEIN DETERMINATION; PROTEIN GLYCOSYLATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN TRANSPORT; REACTION ANALYSIS; SIALYLATION;

ANIMALS; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; GLYCOSYLATION; HUMANS; INTERFERON TYPE II; POLYSACCHARIDES; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPODOPTERA;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; INSECTA; MAMMALIA; UNIDENTIFIED BACULOVIRUS;

EID: 0033526537     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990605)63:5<559::AID-BIT6>3.0.CO;2-L     Document Type: Article

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