Influence of intracellular nucleotide and nucleotide sugar contents on recombinant interferon-γ glycosylation during batch and fed-batch cultures of CHO cells

Biotechnology and Bioengineering

Volumn 100, Issue 4, 2008, Pages 721-733

  Kochanowski, N ^a   Blanchard, F ^a   Cacan, R ^b   Chirat, F ^b   Guedon, E ^a   Marc, A ^a   Goergen, J L ^a  

^a UNIVERSITÉ DE LORRAINE   (France)

^b UNIVERSITÉ DES SCIENCES ET TECHNOLOGIES DE LILLE   (France)

Author keywords

CHO cells; Energetic status; Glycosylation macroheterogeneity; IFN 7; Nucleotide sugars

Indexed keywords

BATCH CELL CULTURE; BATCH DATA PROCESSING; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; CELLS; ESTERIFICATION; GLYCOSYLATION; INTERNET PROTOCOLS; NUCLEIC ACIDS; NUCLEOTIDES; SUGAR (SUCROSE); SUGARS; SYNTHESIS (CHEMICAL);

(E ,3E) PROCESS; ADENYLATE ENERGY CHARGE (AEC); BATCH CULTURES; BATCH PROCESSING; CHINESE HAMSTER OVARY (CHO) CELLS; FED BATCH CULTURES; FED BATCHES; GLYCOFORMS; GLYCOSYLATED; INTRACELLULAR LEVELS; NUCLEOTIDE SUGARS; SUGAR NUCLEOTIDES; SYNTHESIS (OF CHIRAL IONIC LIQUIDS);

CELL CULTURE;

ADENOSINE PHOSPHATE; NUCLEOTIDE; RECOMBINANT GAMMA INTERFERON; URIDINE DIPHOSPHATE GALACTOSE; URIDINE DIPHOSPHATE GLUCOSE; URIDINE TRIPHOSPHATE;

ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; BIOREACTOR; CELL GROWTH; CELL METABOLISM; CELL VIABILITY; CHO CELL; CULTURE MEDIUM; ENERGY TRANSFER; FED BATCH CULTURE; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; SERUM;

ANIMALS; CARBOHYDRATE METABOLISM; CELL CULTURE TECHNIQUES; CELL LINE, TRANSFORMED; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA, SERUM-FREE; GALACTOSE; GLYCOSYLATION; INTERFERON-GAMMA, RECOMBINANT; SERUM; SIALYLTRANSFERASES; URIDINE DIPHOSPHATE; URIDINE TRIPHOSPHATE;

EID: 46249121065     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21816     Document Type: Article

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